scholarly journals Life-history changes in the cold tolerance of the two-spot spider mite Tetranychus urticae : applications in pest control and establishment risk assessment

2018 ◽  
Vol 43 (4) ◽  
pp. 334-345 ◽  
Author(s):  
Nicola White ◽  
Jeffrey S. Bale ◽  
Scott A. L. Hayward
Keyword(s):  
Risk Assessment ◽  
Life History ◽  
Cold Tolerance ◽  
Tetranychus Urticae ◽  
Pest Control ◽  
Spider Mite

scholarly journals Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Nicolas Bensoussan ◽  
Sameer Dixit ◽  
Midori Tabara ◽  
David Letwin ◽  
Maja Milojevic ◽  
...  
Keyword(s):  
Rna Interference ◽  
Tetranychus Urticae ◽  
Pest Control ◽  
Spider Mite ◽  
26S Proteasome ◽  
Rnai Pathway ◽  
Rnai Machinery ◽  
Two Spotted Spider Mite ◽  
Species Specific

Abstract Comprehensive understanding of pleiotropic roles of RNAi machinery highlighted the conserved chromosomal functions of RNA interference. The consequences of the evolutionary variation in the core RNAi pathway genes are mostly unknown, but may lead to the species-specific functions associated with gene silencing. The two-spotted spider mite, Tetranychus urticae, is a major polyphagous chelicerate pest capable of feeding on over 1100 plant species and developing resistance to pesticides used for its control. A well annotated genome, susceptibility to RNAi and economic importance, make T. urticae an excellent candidate for development of an RNAi protocol that enables high-throughput genetic screens and RNAi-based pest control. Here, we show that the length of the exogenous dsRNA critically determines its processivity and ability to induce RNAi in vivo. A combination of the long dsRNAs and the use of dye to trace the ingestion of dsRNA enabled the identification of genes involved in membrane transport and 26S proteasome degradation as sensitive RNAi targets. Our data demonstrate that environmental RNAi can be an efficient reverse genetics and pest control tool in T. urticae. In addition, the species-specific properties together with the variation in the components of the RNAi machinery make T. urticae a potent experimental system to study the evolution of RNAi pathways.

scholarly journals Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response

2020 ◽  
Author(s):  
Nicolas Bensoussan ◽  
Sameer Dixit ◽  
Midori Tabara ◽  
David Letwin ◽  
Maja Milojevic ◽  
...  
Keyword(s):  
Rna Interference ◽  
Tetranychus Urticae ◽  
Pest Control ◽  
Spider Mite ◽  
26S Proteasome ◽  
Rnai Pathway ◽  
Rnai Machinery ◽  
Two Spotted Spider Mite ◽  
Species Specific

AbstractComprehensive understanding of pleiotropic roles of RNAi machinery highlighted the conserved chromosomal functions of RNA interference. The consequences of the evolutionary variation in the core RNAi pathway genes are mostly unknown, but may lead to the species-specific functions associated with gene silencing. The two-spotted spider mite, Tetranychus urticae, is a major polyphagous chelicerate pest capable of feeding on over 1,100 plant species and developing resistance to pesticides used for its control. A well annotated genome, susceptibility to RNAi and economic importance, make T. urticae an excellent candidate for development of an RNAi protocol that enables high-throughput genetic screens and RNAi-based pest control. Here, we show that the length of the exogenous dsRNA critically determines its processivity and ability to induce RNAi in vivo. A combination of the long dsRNAs and the use of dye to trace the ingestion of dsRNA enabled the identification of genes involved in membrane transport and 26S proteasome degradation as sensitive RNAi targets. Our data demonstrate that environmental RNAi can be an efficient reverse genetics and pest control tool in T. urticae. In addition, the species-specific properties together with the variation in the components of the RNAi machinery make T. urticae a potent experimental system to study the evolution of RNAi pathways.

scholarly journals Effects of host plant on life‐history traits in the polyphagous spider mite Tetranychus urticae

2015 ◽  
Vol 5 (15) ◽  
pp. 3151-3158 ◽  
Author(s):  
Cassandra Marinosci ◽  
Sara Magalhães ◽  
Emilie Macke ◽  
Maria Navajas ◽  
David Carbonell ◽  
...  
Keyword(s):  
Life History ◽  
Host Plant ◽  
Tetranychus Urticae ◽  
Spider Mite ◽  
Life History Traits

scholarly journals THE EFFICIENCY OF BIOLOGICAL PREPARATION ACTOFIT APPLICATION ON HOP AGAINST SPIDER MITE

2010 ◽  
Vol 11 ◽  
pp. 102-108
Author(s):  
V.M. Venger ◽  
N.A. Lukashevich ◽  
I.V. Jakubenko ◽  
O.V. Venger ◽  
N.A. Fedorchuk
Keyword(s):  
Control System ◽  
Tetranychus Urticae ◽  
Pest Control ◽  
Spider Mite ◽  
Chemical Preparation ◽  
High Quality ◽  
Vegetative Period ◽  
Biological Preparation ◽  
Pesticide Load ◽  
Tetranychus Urticae Koch

Spider mite (Tetranychus urticae Koch.). is the most harmful hop pest during the vegetative period. Pest control system foresees 6-8 treatments by different chemical preparations against him. The application of biological preparation Actofit had essentially decreased the pesticide load on hop agricoenosis and had improved the environment situation. Spraying of hop gardens against the spider mite had enabled receipt of high quality cones crop at level 8,2-10,3 c/ha in dependence on doze of preparation which was at level chemical preparation Demitan by quantitative and qualitative indices.

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