scholarly journals First report of successful transplantation of a pediatric donor liver graft after hypothermic machine perfusion

2019 ◽  
Vol 23 (3) ◽  
Author(s):  
Maureen J. M. Werner ◽  
Otto B. Leeuwen ◽  
Iris E. M. Jong ◽  
Frank A. J. A. Bodewes ◽  
Masato Fujiyoshi ◽  
...  
Keyword(s):  
Liver Graft ◽  
Machine Perfusion ◽  
Donor Liver ◽  
First Report ◽  
Hypothermic Machine Perfusion ◽  
Successful Transplantation

scholarly journals Graft protection of the liver by hypothermic machine perfusion involves recovery of graft regeneration in rats

2019 ◽  
Vol 47 (1) ◽  
pp. 427-437 ◽  
Author(s):  
Jun-Jun Jia ◽  
Hai-Yang Xie ◽  
Jian-Hui Li ◽  
Yong He ◽  
Li Jiang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Western Blot ◽  
Cyclin D1 ◽  
Liver Graft ◽  
Enzyme Release ◽  
Machine Perfusion ◽  
Cyclin E1 ◽  
Cell Cycle Genes ◽  
Hypothermic Machine Perfusion ◽  
The Impact

Objective This study was performed to evaluate the impact and underlying mechanisms of hypothermic machine perfusion (HMP) on half-size liver graft regeneration. Methods Forty rats were randomly assigned to five groups: two in vitro groups (static cold storage [SCS] and HMP) and three in vivo groups (orthotopic liver transplantation, SCS, and HMP). Perfusates and plasma samples were collected for analysis of hepatic enzymes. Liver tissue was obtained for evaluation of histology, immunohistochemistry (Ki67 and proliferating cell nuclear antigen [PCNA]), and the regeneration rate. Cell cycle genes were analyzed by quantitative real-time polymerase chain reaction, and cyclin D1 and cyclin E1 were semiquantified by western blot. Results HMP improved histopathological outcomes and decreased hepatic enzyme release. The expression of Ki67 and PCNA demonstrated a greater proliferation activity in the HMP than SCS group, and the expression of almost all cell cycle genes was elevated following HMP. Western blot results showed higher protein levels of cyclin D1 and cyclin E1 in the HMP than SCS group. Conclusions Our findings suggest for the first time that half-size liver graft protection by HMP involves recovery of graft regeneration.

scholarly journals Optimizing porcine donor kidney preservation with normothermic or hypothermic machine perfusion: A systematic review

2021 ◽  
Author(s):  
Sarah Bouari ◽  
Özgür Eryigit ◽  
Ron W. F. Bruin ◽  
Jan N. M. IJzermans ◽  
Robert C. Minnee
Keyword(s):  
Systematic Review ◽  
Machine Perfusion ◽  
Donor Kidney ◽  
Kidney Preservation ◽  
Hypothermic Machine Perfusion

scholarly journals Early graft calcification without graft dysfunction after living donor liver transplantation: two case reports

2021 ◽  
Vol 14 (5) ◽  
pp. 1491-1495
Author(s):  
Peilin Li ◽  
Masaaki Hidaka ◽  
Yu Huang ◽  
Takanobu Hara ◽  
Kantoku Nagakawa ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Hepatic Artery ◽  
Living Donor ◽  
Living Donor Liver Transplantation ◽  
Liver Graft ◽  
Graft Dysfunction ◽  
Donor Liver ◽  
Donor Liver Transplantation ◽  
First Case ◽  
Post Operation

AbstractGraft calcification after liver transplantation (LT) has seldom been reported, but almost of all previously reported cases have been attributed to graft dysfunction. We herein report two cases of graft calcification without liver dysfunction after living donor liver transplantation (LDLT). Two patients who underwent LDLT were found to have graft calcification in the early postoperative period (< 1 month). Calcification in the first case was found at the cut edge of the liver at post-operative day (POD) 10, showing a time-dependent increase in calcification severity. The second patient underwent hepatic artery re-anastomosis due to hepatic artery thrombosis on POD4 and received balloon-occluded retrograde transvenous obliteration of the splenic kidney shunt due to decreased portal vein blood flow on POD6. She was found to have diffuse hepatic calcification in the distant hepatic artery area at 1-month post-operation followed by gradual graft calcification at the resection margin at 6-month post-operation. Neither case showed post-operative graft dysfunction. Calcification of the liver graft after LDLT is likely rare, and graft calcification does not seem to affect the short-term liver function in LDLT cases. We recommend strictly controlling the warm/cold ischemia time and reducing the physical damage to the donor specimen as well as monitoring for early calcification by computed tomography.

Atrial septal defect occlusion by a catheter during reperfusion of homograft in a case of living donor liver transplantation

2021 ◽  
pp. 1-2
Author(s):  
Shiro Baba ◽  
Takuya Hirata ◽  
Hideaki Okajima
Keyword(s):  
Liver Transplantation ◽  
Atrial Septal Defect ◽  
Living Donor ◽  
Living Donor Liver Transplantation ◽  
Septal Defect ◽  
Liver Graft ◽  
Pulmonary Artery Stenosis ◽  
Donor Liver ◽  
Easy Method ◽  
Donor Liver Transplantation

Abstract Liver transplantation for patients with atrial septal defect and pulmonary artery stenosis, causing high right atrium pressure, raises concerns about embolism in systemic vessels during reperfusion of the donor liver graft. Temporal atrial septal defect occlusion by a catheter is a simple and easy method of preventing the complication.

scholarly journals Is Hepatocyte Necrosis a Good Marker of Donor Liver Viability During Machine Perfusion?

2021 ◽  
Author(s):  
Desley A.H. Neil ◽  
Hynek Mergental ◽  
Angus Hann ◽  
Richard W Laing ◽  
Hermien Hartog ◽  
...  
Keyword(s):  
Machine Perfusion ◽  
Donor Liver ◽  
Good Marker ◽  
Hepatocyte Necrosis ◽  
Liver Viability

Hypothermic machine perfusion of rat livers preserves endothelial cell function

2005 ◽  
Vol 37 (1) ◽  
pp. 335-337 ◽  
Author(s):  
H. Xu ◽  
J.X. Zhang ◽  
J.W. Jones ◽  
J.H. Southard ◽  
M.G. Clemens ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Function ◽  
Machine Perfusion ◽  
Endothelial Cell Function ◽  
Hypothermic Machine Perfusion ◽  
Rat Livers

Combined Heart and Kidney Transplantation: How Hypothermic Machine Perfusion Enables Delayed Kidney Implantation

2021 ◽  
Author(s):  
S. Lukas ◽  
C. Bogensperger ◽  
H. Antretter ◽  
M. Grimm ◽  
S. Schneeberger ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
Machine Perfusion ◽  
Hypothermic Machine Perfusion
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