Induced pluripotent stem cell‐derived myeloid cells expressing OX40 ligand amplify antigen‐specific T cells in advanced melanoma

2020 ◽  
Vol 33 (5) ◽  
pp. 744-755
Author(s):  
Toshihiro Kimura ◽  
Satoshi Fukushima ◽  
Etsuko Okada ◽  
Haruka Kuriyama ◽  
Hisashi Kanemaru ◽  
...  
Keyword(s):  
T Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Myeloid Cells ◽  
Induced Pluripotent Stem Cell ◽  
Advanced Melanoma ◽  
Ox40 Ligand ◽  
Induced Pluripotent

scholarly journals Differential Transgene Silencing of Myeloid-Specific Promoters in the AAVS1 Safe Harbor Locus of Induced Pluripotent Stem Cell-Derived Myeloid Cells

2020 ◽  
Vol 31 (3-4) ◽  
pp. 199-210 ◽  
Author(s):  
Denise Klatt ◽  
Erica Cheng ◽  
Dirk Hoffmann ◽  
Giorgia Santilli ◽  
Adrian J. Thrasher ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Myeloid Cells ◽  
Induced Pluripotent Stem Cell ◽  
Transgene Silencing ◽  
Safe Harbor ◽  
Induced Pluripotent

scholarly journals Induced pluripotent stem cell macrophages present antigen to proinsulin-specific T cell receptors from donor-matched islet-infiltrating T cells in type 1 diabetes

Diabetologia ◽  
2019 ◽  
Vol 62 (12) ◽  
pp. 2245-2251 ◽  
Author(s):  
Kriti Joshi ◽  
Colleen Elso ◽  
Ali Motazedian ◽  
Tanya Labonne ◽  
Jacqueline V. Schiesser ◽  
...  
Keyword(s):  
T Cells ◽  
Type 1 Diabetes ◽  
Stem Cell ◽  
T Cell ◽  
T Cell Receptors ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Cell Receptors ◽  
Induced Pluripotent

Abstract Aims/hypothesis Type 1 diabetes is an autoimmune disorder characterised by loss of insulin-producing beta cells of the pancreas. Progress in understanding the cellular and molecular mechanisms underlying the human disease has been hampered by a dearth of appropriate human experimental models. We previously reported the characterisation of islet-infiltrating CD4+ T cells from a deceased organ donor who had type 1 diabetes. Methods Induced pluripotent stem cell (iPSC) lines derived from the above donor were differentiated into CD14+ macrophages and tested for their capacity to present antigen to T cell receptors (TCRs) derived from islet-infiltrating CD4+ T cells from the same donor. Results The iPSC macrophages displayed typical macrophage morphology, surface markers (CD14, CD86, CD16 and CD11b) and were phagocytic. In response to IFNγ treatment, iPSC macrophages upregulated expression of HLA class II, a characteristic that correlated with their capacity to present epitopes derived from proinsulin C-peptide to a T cell line expressing TCRs derived from islet-infiltrating CD4+ T cells of the original donor. T cell activation was specifically blocked by anti-HLA-DQ antibodies but not by antibodies directed against HLA-DR. Conclusions/interpretation This study provides a proof of principle for the use of iPSC-derived immune cells for modelling key cellular interactions in human type 1 diabetes.

Use of 3D culture systems to generate human induced pluripotent stem cell-derived [beta]-cells in vitro

2018 ◽  
Author(s):  
Fantuzzi Federica ◽  
Toivonen Sanna ◽  
Schiavo Andrea Alex ◽  
Pachera Nathalie ◽  
Rajaei Bahareh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Beta Cells ◽  
Pluripotent Stem Cell ◽  
3D Culture ◽  
Induced Pluripotent Stem Cell ◽  
Culture Systems ◽  
Induced Pluripotent
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