scholarly journals Deciphering the metabolic pathways influencing heat and cold responses during post-harvest physiology of peach fruit

2013 ◽  
Vol 37 (3) ◽  
pp. 601-616 ◽  
Author(s):  
MARTIN A. LAUXMANN ◽  
JULIA BORSANI ◽  
SONIA OSORIO ◽  
VERÓNICA A. LOMBARDO ◽  
CLAUDIO O. BUDDE ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
Peach Fruit ◽  
Post Harvest ◽  
Cold Responses

Effect of biodegradable chitosan-rice-starch nanocomposite films on post-harvest quality of stored peach fruit

2017 ◽  
Vol 69 (1-2) ◽  
pp. 1600208 ◽  
Author(s):  
Manpreet Kaur ◽  
Anu Kalia ◽  
Anirudh Thakur
Keyword(s):  
Nanocomposite Films ◽  
Rice Starch ◽  
Peach Fruit ◽  
Post Harvest

Effect of post-harvest heat treatment on proteome change of peach fruit during ripening

2011 ◽  
Vol 74 (7) ◽  
pp. 1135-1149 ◽  
Author(s):  
Li Zhang ◽  
Zhifang Yu ◽  
Li Jiang ◽  
Juan Jiang ◽  
Haibo Luo ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Peach Fruit ◽  
Post Harvest

scholarly journals Subcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism

2019 ◽  
Author(s):  
Sophie Trefely ◽  
Joyce Liu ◽  
Katharina Huber ◽  
Mary T. Doan ◽  
Helen Jiang ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Metabolic Activity ◽  
Metabolic Pathways ◽  
Subcellular Fractionation ◽  
Clear Understanding ◽  
Isotope Tracing ◽  
Dynamic Regulation ◽  
Whole Cells ◽  
Post Harvest ◽  
Biochemical Fractionation

AbstractOBJECTIVEThe dynamic regulation of metabolic pathways can be monitored by stable isotope tracing. Yet, many metabolites are part of distinct processes within different subcellular compartments. Standard isotope tracing experiments relying on analyses in whole cells may not accurately reflect compartmentalized metabolic processes. Analysis of compartmentalized metabolism and the dynamic interplay between compartments can potentially be achieved by stable isotope tracing followed by subcellular fractionation. Although it is recognized that metabolism can take place during biochemical fractionation of cells, a clear understanding of how such post-harvest metabolism impacts the interpretation of subcellular isotope tracing data and methods to correct for this are lacking. We set out to directly assess artifactual metabolism, enabling us to develop and test strategies to correct for it. We apply these techniques to examine the compartment-specific metabolic kinetics of 13C-labeled substrates targeting central metabolic pathways.METHODSWe designed a stable isotope tracing strategy to interrogate post-harvest metabolic activity during subcellular fractionation using liquid chromatography-mass spectrometry (LC-MS).RESULTSWe show that post-harvest metabolic activity occurs rapidly (within seconds) upon cell harvest. With further characterization we reveal that this post-harvest metabolism is enzymatic, and reflects the metabolic capacity of the sub-cellular compartment analyzed; but is limited in the extent of its propagation into downstream metabolites in metabolic pathways. We also propose and test a post-labeling strategy to assess the amount of post-harvest metabolism occurring in an experiment and then to adjust data to account for this. We validate this approach for both mitochondrial and cytosolic metabolic analyses.CONCLUSIONSOur data indicate that isotope tracing coupled with sub-cellular fractionation can reveal distinct and dynamic metabolic features of cellular compartments, and that confidence in such data can be improved by applying a post-labeling correction strategy. We examine compartmentalized metabolism of acetate and glutamine and show that acetyl-CoA is turned over rapidly in the cytosol and acts as a pacemaker of anabolic metabolism in this compartment.

scholarly journals Post-harvest UV-B radiation modulates metabolite profile in peach fruit

2018 ◽  
Vol 139 ◽  
pp. 127-134 ◽  
Author(s):  
M. Santin ◽  
L. Lucini ◽  
A. Castagna ◽  
G. Chiodelli ◽  
M-T. Hauser ◽  
...  
Keyword(s):  
Metabolite Profile ◽  
Peach Fruit ◽  
Post Harvest

scholarly journals Metabolomic profiling of matured coconut water during post-harvest storage revealed discrimination and distinct changes in metabolites

RSC Advances ◽  
2018 ◽  
Vol 8 (55) ◽  
pp. 31396-31405 ◽  
Author(s):  
Weijun Chen ◽  
Guanfei Zhang ◽  
Wenxue Chen ◽  
Qiuping Zhong ◽  
Haiming Chen
Keyword(s):  
Statistical Analysis ◽  
Multivariate Statistical Analysis ◽  
Metabolic Pathways ◽  
Room Temperature ◽  
Coconut Water ◽  
Multivariate Statistical ◽  
Post Harvest ◽  
Metabolomic Profiling

The metabolites of coconut water stored at room temperature were analyzed using UPLC-MS/MS and multivariate statistical analysis to identify the differential biomarkers and metabolic pathways during post-harvest storage.

Post-harvest 1-methylcyclopropene and ethephon treatments differently modify protein profiles of peach fruit during ripening

2012 ◽  
Vol 48 (2) ◽  
pp. 609-619 ◽  
Author(s):  
Li Zhang ◽  
Li Jiang ◽  
Yun Shi ◽  
Haibo Luo ◽  
Ruoyi Kang ◽  
...  
Keyword(s):  
Protein Profiles ◽  
Peach Fruit ◽  
Post Harvest ◽  
Modify Protein

NON-DESTRUCTIVE EVALUATION OF POST-HARVEST PEACH FRUIT SOFTENING

2004 ◽  
pp. 269-274 ◽  
Author(s):  
R. Scorza ◽  
W. Anger ◽  
D. Peterson ◽  
K. Bett ◽  
E. Champagne ◽  
...  
Keyword(s):  
Fruit Softening ◽  
Peach Fruit ◽  
Post Harvest ◽  
Non Destructive Evaluation ◽  
Non Destructive
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