Generation and analysis of expressed sequence tag sequences from a soft-seeded pomegranate cDNA library

2017 ◽  
Vol 136 (6) ◽  
pp. 994-999 ◽  
Author(s):  
Shuiming Zhang ◽  
Lei Chen ◽  
Rong Huang ◽  
Abdurazak Isha ◽  
Lili Dong
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tag ◽  
Expressed Sequence

scholarly journals Analysis of Expressed Sequence Tag Data and Gene Expression Profiles Involved in Conidial Germination of Fusarium oxysporum

2006 ◽  
Vol 72 (2) ◽  
pp. 1667-1671 ◽  
Author(s):  
Ye Deng ◽  
Haitao Dong ◽  
Qingchao Jin ◽  
Cheng'en Dai ◽  
Yongqi Fang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Synthesis ◽  
Fusarium Oxysporum ◽  
Cdna Library ◽  
Expressed Sequence Tag ◽  
Expression Profiles ◽  
Cdna Array ◽  
Gene Expression Profiles ◽  
Conidial Germination ◽  
Expressed Sequence

ABSTRACT We obtained 3,372 tentative unique transcripts (TUTs) from a cDNA library of Fusarium oxysporum. A cDNA array with 3,158 TUTs was produced to analyze gene expression profiles in conidial germination. It seems that ras and other signaling genes, e.g., ccg, cooperatively initiate conidial germination in Fusarium by increasing protein synthesis.

An expressed sequence tag analysis of a full-length, spliced-leader cDNA library from Leishmania major promastigotes

1996 ◽  
Vol 76 (1-2) ◽  
pp. 345-348 ◽  
Author(s):  
Mark P. Levick ◽  
Jenefer M. Blackwell ◽  
Vivienne Connor ◽  
Richard M.R. Coulson ◽  
Alistair Miles ◽  
...  
Keyword(s):  
Cdna Library ◽  
Leishmania Major ◽  
Expressed Sequence Tag ◽  
Full Length ◽  
Spliced Leader ◽  
Expressed Sequence Tag Analysis ◽  
Expressed Sequence

Construction of cDNA library from Prunus campanulata leaves and preliminary expressed sequence tag (EST) analysis during cold stress

Biologia ◽  
2015 ◽  
Vol 70 (8) ◽  
Author(s):  
Ying-Hui Zhang ◽  
Jun-Dong Rong ◽  
Li-Guang Chen ◽  
Ling-Yan Chen ◽  
Tian-You He ◽  
...  
Keyword(s):  
Gene Expression ◽  
Low Temperature ◽  
Cold Stress ◽  
Cdna Library ◽  
Molecular Mechanisms ◽  
Expressed Sequence Tag ◽  
Go Annotation ◽  
Est Analysis ◽  
Young Leaves ◽  
Expressed Sequence

AbstractThe molecular mechanisms underlying cold-resistance in Prunus campanulata Maxim. (P. campanulata) are not fully understood. This study aimed to establish a full-length library and analyze expressed sequence tags (ESTs) to provide tools to investigate the mechanisms of P. campanulata growth at low temperatures. Based on the switching mechanism at 5’end of RNA transcript technology, a full-1ength cDNA library was generated from young leaves of P. campanulata after 72 h treatment at 1◦C, and a preliminary EST analysis was carried out. Quantitative reverse transcription polymerase chain reaction was used to assess the expression of selected cold-related genes. The cDNA library titer was 1.2 × 106 cfu/mL−1, with a recombinant rate of 96%. The average size of inserted cDNA fragments was 1.3 Kb. EST data revealed the existence of 834 clones representing a total of 667 unigenes, including 574 singletons and 93 contigs. Blast analysis identified 475 unigenes with known and putative functions. Based on similarity search and GO annotation, 84 unigenes were associated with “response to stimuli”, suggesting that cold stress induced significant alterations in gene expression in P. campanulata cultivated at 1◦C for 72 h. Interestingly, DRP, MYB, HSP, GPX and GA20-ox gene expression was significantly up-regulated in plants cultivated at low temperature, while transcript levels of TIL, CDPK were decreased. P. campanulata cultivating at low temperature express genes associated with “response to stimuli”, and in particular DRP, MYB, HSP, GPX and GA20-ox gene are up-regulated while TIL, CDPK are downregulated in response to low temperature-stress

scholarly journals A full-length enriched cDNA library and expressed sequence tag analysis of the parasitic weed, Striga hermonthica

2010 ◽  
Vol 10 (1) ◽  
pp. 55 ◽  
Author(s):  
Satoko Yoshida ◽  
Juliane K Ishida ◽  
Nasrein M Kamal ◽  
Abdelbagi M Ali ◽  
Shigetou Namba ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tag ◽  
Full Length ◽  
Striga Hermonthica ◽  
Parasitic Weed ◽  
Expressed Sequence Tag Analysis ◽  
Expressed Sequence

Analysis of expressed sequence tags from a naked foraminiferan Reticulomyxa filosa

Genome ◽  
2006 ◽  
Vol 49 (8) ◽  
pp. 882-887 ◽  
Author(s):  
Fabien Burki ◽  
Sergey I Nikolaev ◽  
Ignacio Bolivar ◽  
Jackie Guiard ◽  
Jan Pawlowski
Keyword(s):  
Cluster Analysis ◽  
Calcium Carbonate ◽  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Expressed Sequence Tag ◽  
Phylogenetic Group ◽  
Protein Database ◽  
Est Database ◽  
Conserved Genes ◽  
Expressed Sequence

Foraminifers are a major component of modern marine ecosystems and one of the most important oceanic producers of calcium carbonate. They are a key phylogenetic group among amoeboid protists, but our knowledge of their genome is still mostly limited to a few conserved genes. Here, we report the first study of expressed genes by means of expressed sequence tag (EST) from the freshwater naked foraminiferan Reticulomyxa filosa. Cluster analysis of 1630 valid ESTs enabled the identification of 178 groups of related sequences and 871 singlets. Approximately 50% of the putative unique 1059 ESTs could be annotated using Blast searches against the protein database SwissProt + TrEMBL. The EST database described here is the first step towards gene discovery in Foraminifera and should provide the basis for new insights into the genomic and transcriptomic characteristics of these interesting but poorly understood protists.Key words: Rhizaria, Foraminifera, cDNA library, annotation.

Expressed sequence tag (EST) analysis of a Schistosomajaponicum cercariae cDNA library

Acta Tropica ◽  
2002 ◽  
Vol 82 (2) ◽  
pp. 215-224 ◽  
Author(s):  
Ming-Chiu Fung ◽  
Man-Tat Lau ◽  
Xiao-Guang Chen
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tag ◽  
Est Analysis ◽  
Expressed Sequence

scholarly journals Chromosomal localization and expressed sequence tag generation of clones from a normalized human adult thymus cDNA library.

1995 ◽  
Vol 5 (4) ◽  
pp. 359-367 ◽  
Author(s):  
J E Lamerdin ◽  
R S Athwal ◽  
M S Kansara ◽  
A K Sandhu ◽  
S R Patanjali ◽  
...  
Keyword(s):  
Cdna Library ◽  
Chromosomal Localization ◽  
Expressed Sequence Tag ◽  
Human Adult ◽  
Expressed Sequence ◽  
Adult Thymus
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