Analysis of expressed sequence tags from a naked foraminiferan Reticulomyxa filosa

Genome ◽  
2006 ◽  
Vol 49 (8) ◽  
pp. 882-887 ◽  
Author(s):  
Fabien Burki ◽  
Sergey I Nikolaev ◽  
Ignacio Bolivar ◽  
Jackie Guiard ◽  
Jan Pawlowski
Keyword(s):  
Cluster Analysis ◽  
Calcium Carbonate ◽  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Expressed Sequence Tag ◽  
Phylogenetic Group ◽  
Protein Database ◽  
Est Database ◽  
Conserved Genes ◽  
Expressed Sequence

Foraminifers are a major component of modern marine ecosystems and one of the most important oceanic producers of calcium carbonate. They are a key phylogenetic group among amoeboid protists, but our knowledge of their genome is still mostly limited to a few conserved genes. Here, we report the first study of expressed genes by means of expressed sequence tag (EST) from the freshwater naked foraminiferan Reticulomyxa filosa. Cluster analysis of 1630 valid ESTs enabled the identification of 178 groups of related sequences and 871 singlets. Approximately 50% of the putative unique 1059 ESTs could be annotated using Blast searches against the protein database SwissProt + TrEMBL. The EST database described here is the first step towards gene discovery in Foraminifera and should provide the basis for new insights into the genomic and transcriptomic characteristics of these interesting but poorly understood protists.Key words: Rhizaria, Foraminifera, cDNA library, annotation.

Survey of a cDNA library from the turkey (Meleagris gallopavo)

Genome ◽  
2005 ◽  
Vol 48 (1) ◽  
pp. 12-17 ◽  
Author(s):  
L D Chaves ◽  
J A Rowe ◽  
K M Reed
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Expressed Sequence Tags ◽  
Expressed Sequence Tag ◽  
Meleagris Gallopavo ◽  
Whole Genome Sequence ◽  
Snp Discovery ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Important Species ◽  
Expressed Sequence

Genome characterization and analysis is an imperative step in identifying and selectively breeding for improved traits of agriculturally important species. Expressed sequence tags (ESTs) represent a transcribed portion of the genome and are an effective way to identify genes within a species. Downstream applications of EST projects include DNA microarray construction and interspecies comparisons. In this study, 694 ESTs were sequenced and analyzed from a library derived from a 24-day-old turkey embryo. The 437 unique sequences identified were divided into 76 assembled contigs and 361 singletons. The majority of significant comparative matches occurred between the turkey sequences and sequences reported from the chicken. Whole genome sequence from the chicken was used to identify potential exon–intron boundaries for selected turkey clones and intron-amplifying primers were developed for sequence analysis and single nucleotide polymorphism (SNP) discovery. Identified SNPs were genotyped for linkage analysis on two turkey reference populations. This study significantly increases the number of EST sequences available for the turkey.Key words: turkey, cDNA, expressed sequence tag, single nucleotide polymorphism.

scholarly journals Analysis of Expressed Sequence Tag Data and Gene Expression Profiles Involved in Conidial Germination of Fusarium oxysporum

2006 ◽  
Vol 72 (2) ◽  
pp. 1667-1671 ◽  
Author(s):  
Ye Deng ◽  
Haitao Dong ◽  
Qingchao Jin ◽  
Cheng'en Dai ◽  
Yongqi Fang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Synthesis ◽  
Fusarium Oxysporum ◽  
Cdna Library ◽  
Expressed Sequence Tag ◽  
Expression Profiles ◽  
Cdna Array ◽  
Gene Expression Profiles ◽  
Conidial Germination ◽  
Expressed Sequence

ABSTRACT We obtained 3,372 tentative unique transcripts (TUTs) from a cDNA library of Fusarium oxysporum. A cDNA array with 3,158 TUTs was produced to analyze gene expression profiles in conidial germination. It seems that ras and other signaling genes, e.g., ccg, cooperatively initiate conidial germination in Fusarium by increasing protein synthesis.

scholarly journals Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

2014 ◽  
Vol 13 (2) ◽  
pp. 378-386
Author(s):  
Su-mei ZHAO ◽  
Yong-gang LIU ◽  
Hong-bing PAN ◽  
Xi ZHANG ◽  
Chang-rong GE ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Full Length ◽  
Full Length Cdna ◽  
Expressed Sequence

Exploitation of a turbot ( Scophthalmus maximus L.) immune‐related expressed sequence tag (EST) database for microsatellite screening and validation

2012 ◽  
Vol 12 (4) ◽  
pp. 706-716 ◽  
Author(s):  
R. NAVAJAS‐PÉREZ ◽  
F. ROBLES ◽  
M. J. MOLINA‐LUZÓN ◽  
R. De La HERRÁN ◽  
J. A. ÁLVAREZ‐DIOS ◽  
...  
Keyword(s):  
Expressed Sequence Tag ◽  
Scophthalmus Maximus ◽  
Est Database ◽  
Turbot Scophthalmus Maximus ◽  
Expressed Sequence

scholarly journals Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening

BMC Genomics ◽  
2008 ◽  
Vol 9 (1) ◽  
pp. 351 ◽  
Author(s):  
Ross N Crowhurst ◽  
Andrew P Gleave ◽  
Elspeth A MacRae ◽  
Charles Ampomah-Dwamena ◽  
Ross G Atkinson ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Gene Discovery ◽  
Est Database ◽  
Expressed Sequence
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