Oxidative stress in human gingival fibroblasts from periodontitis versus healthy counterparts

Oral Diseases ◽  
2021 ◽  
Author(s):  
Jia Liu ◽  
Xiaoxuan Wang ◽  
Ming Zheng ◽  
Qingxian Luan
Keyword(s):  
Oxidative Stress ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts

The Upregulation of Transglutaminase-2 by Cyclosporin A in Human Gingival Fibroblasts Is Augmented by Oxidative Stress

2013 ◽  
Vol 84 (10) ◽  
pp. 1469-1475 ◽  
Author(s):  
Shiuan-Shinn Lee ◽  
Chung-Hung Tsai ◽  
Yu-Hsiang Kuan ◽  
Fu-Mei Huang ◽  
Yu-Chao Chang
Keyword(s):  
Oxidative Stress ◽  
Cyclosporin A ◽  
Transglutaminase 2 ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts

scholarly journals Cytotoxic and Genotoxic Effects of Composite Resins on Cultured Human Gingival Fibroblasts

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5225
Author(s):  
Francesco De Angelis ◽  
Domitilla Mandatori ◽  
Valeria Schiavone ◽  
Francesco Paolo Melito ◽  
Silvia Valentinuzzi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Activity ◽  
Triethylene Glycol ◽  
Composite Resins ◽  
Dental Composite ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cell Metabolic Activity ◽  
Genotoxic Potential

The aim of the study was to evaluate the cytotoxic and genotoxic potential of five commercially available dental composite resins (CRs), investigating the effect of their quantifiable bisphenol-A-glycidyl-methacrylate (Bis-GMA) and/or triethylene glycol dimethacrylate (TEGDMA) release. Experiments were performed using the method of soaking extracts, which were derived from the immersion of the following CRs in the culture medium: Clearfil-Majesty-ES-2, GrandioSO, and Enamel-plus-HRi (Bis-GMA-based); Enamel-BioFunction and VenusDiamond (Bis-GMA-free). Human Gingival Fibroblasts (hGDFs) were employed as the cellular model to mimic in vitro the oral cavity milieu, where CRs simultaneously release various components. Cell metabolic activity, oxidative stress, and genotoxicity were used as cellular outcomes. Results showed that only VenusDiamond and Enamel-plus-HRi significantly affected the hGDF cell metabolic activity. In accordance with this, although no CR-derived extract induced a significantly detectable oxidative stress, only VenusDiamond and Enamel-plus-HRi induced significant genotoxicity. Our findings showed, for the CRs employed, a cytotoxic and genotoxic potential that did not seem to depend only on the actual Bis-GMA or TEGDMA content. Enamel-BioFunction appeared optimal in terms of cytotoxicity, and similar findings were observed for Clearfil-Majesty-ES-2 despite their different Bis-GMA/TEGDMA release patterns. This suggested that simply excluding one specific monomer from the CR formulation might not steadily turn out as a successful approach for improving their biocompatibility.

scholarly journals Evaluation of toxicity and response to oxidative stress generated by orthodontic bands in human gingival fibroblasts

2019 ◽  
Vol 90 (2) ◽  
pp. 285-290 ◽  
Author(s):  
Alexandre Marcos Bandeira ◽  
Elizabeth Ferreira Martinez ◽  
Ana Paula Dias Demasi
Keyword(s):  
Oxidative Stress ◽  
Quantitative Polymerase Chain Reaction ◽  
Conditioned Media ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Trypan Blue Exclusion ◽  
Antioxidant Genes ◽  
Glutathione Peroxidase 1 ◽  
Viable Cells ◽  
Polymerase Chain

ABSTRACT Objective: To evaluate the cytotoxicity of stainless-steel orthodontic bands and their influence on the expression of the antioxidant genes in human gingival fibroblasts. Materials and Methods: Ten bands of each brand (Dentsply-Sirona, Dentaurum, TP Orthodontics, and Morelli) were conditioned in 0.2 g/mL culture medium at 37°C for 14 days, and the corresponding conditioned media were applied over the fibroblasts. Cell viability was assessed after 24, 48, and 72 hours of exposure to the conditioned media by trypan blue exclusion assay. Expression of the antioxidant defense genes peroxiredoxin 1 (PRDX1), superoxide dismutase 1 (SOD1), and glutathione peroxidase 1 (GPX1) were evaluated by quantitative polymerase chain reaction after 24 hours of exposure. These parameters were compared to those of the cells not exposed to the conditioned media of the bands (control). Results: All bands promoted a reduction in the number of viable cells in the periods of 48 and 72 hours (P < .01). Analysis of gene expression showed a significant increase in the levels of PRDX1 transcripts caused by the conditioned media of the Dentsply-Sirona, TP Orthodontics, and Morelli bands (P < .01) as well as induction of SOD1 by the conditioned media of the Dentaurum and Morelli (P < .01). Expression of GPX1 was not influenced by the conditioned media. Conclusions: The orthodontic bands showed toxicity to fibroblasts and increased the expression of PRDX1 and SOD1 antioxidant genes, indicating induction of oxidative stress in the cells.

The influence of retraction agents on cytoskeleton reorganization and oxidative stress in primary human gingival fibroblasts (HGFs)

2014 ◽  
Vol 59 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Danuta Nowakowska ◽  
Jolanta Saczko ◽  
Katarzyna Bieżuńska-Kusiak ◽  
Anna Choromańska ◽  
Magda Dubińska-Magiera ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cytoskeleton Reorganization ◽  
And Oxidative Stress

scholarly journals Biological impacts of resveratrol, quercetin, and N-acetylcysteine on oxidative stress in human gingival fibroblasts

2015 ◽  
Vol 56 (3) ◽  
pp. 220-227 ◽  
Author(s):  
Rita Cristina Orihuela-Campos ◽  
Naofumi Tamaki ◽  
Rie Mukai ◽  
Makoto Fukui ◽  
Kaname Miki ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Biological Impacts

scholarly journals Biological Response Induced in Primary Human Gingival Fibroblasts upon Exposure to Various Types of Injectable Astringent Retraction Agents

Materials ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 2081
Author(s):  
Danuta Nowakowska ◽  
Julita Kulbacka ◽  
Joanna Wezgowiec ◽  
Anna Szewczyk ◽  
Dagmara Baczynska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Statistical Significance ◽  
Laser Scanning Microscopy ◽  
Human Gingival Fibroblasts ◽  
Aluminium Chloride ◽  
Confocal Laser ◽  
Rna Expression ◽  
Gingival Fibroblasts ◽  
Gingival Margin

Traditional chemo-mechanical retraction/displacement materials can impact the gingival margin tissues. This study was undertaken to analyze biological responses induced in human gingival fibroblasts (HGFs) upon application of injectable astringent-based agents used in the cordless retraction technique. HGFs were exposed to hemostatic agents (five gels, three pastes, and one foam) based on aluminium chloride, aluminium sulphate and ferric sulphate. Changes in cell viability and proliferation were evaluated using an MTT assay and a BrdU assay. The cytoskeleton structure organization (zyxin and F-actin) was visualized by confocal laser scanning microscopy. Oxidative stress was determined using the Griess Reagent System. The RNA expression levels of antioxidant enzymes were quantified by real-time RT-PCR. The statistical significance was evaluated using Student’s t-test and one-way ANOVA with post-hoc Tukey HSD test. The evaluated agents did not downregulate fibroblast viability or proliferation. No significant cytoskeleton reorganization was observed. Only one agent (Expasyl) induced oxidative stress, demonstrated by the increased level of nitrites. Incubation with the studied agents significantly increased the RNA expression of some antioxidant enzymes (SOD1, SOD3, GPX1). However, no significant influence on the expression of SOD2 and HMOX1 was detected. The injectable forms of chemical retraction agents revealed biocompatibility with HGFs, suggesting their potential clinical usefulness in gingival margin retraction.

TEGDMA induces mitochondrial damage and oxidative stress in human gingival fibroblasts

Biomaterials ◽  
2005 ◽  
Vol 26 (25) ◽  
pp. 5130-5137 ◽  
Author(s):  
Mathieu Lefeuvre ◽  
Wafaa Amjaad ◽  
Michel Goldberg ◽  
Lena Stanislawski
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Damage ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
And Oxidative Stress
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