PTEN regulates proliferation and osteogenesis of dental pulp cells and adipogenesis of human adipose‑derived stem cells

Oral Diseases ◽  
2021 ◽  
Author(s):  
Nunthawan Nowwarote ◽  
Thanaphum Osathanon ◽  
Benjamin P.J. Fournier ◽  
Thanakorn Theerapanon ◽  
Somchai Yodsanga ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Adipose Derived Stem Cells ◽  
Dental Pulp Cells ◽  
Pulp Cells

scholarly journals Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling

RSC Advances ◽  
2015 ◽  
Vol 5 (78) ◽  
pp. 63363-63371 ◽  
Author(s):  
So-Youn Lee ◽  
Hyung-Mun Yun ◽  
Roman A. Perez ◽  
Sara Gallinetti ◽  
Maria-Pau Ginebra ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Dental Pulp ◽  
Integrin Signaling ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Calcium Phosphate Cements ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Stimulation Of

Nanotopological-tailored calcium phosphate cements stimulate odontogenesis of human dental pulp cells through integrin-mediated signaling pathways.

scholarly journals Calcified nodule formation by dental pulp cells derived from rats after subcutaneous injection of an immunosuppressant

2020 ◽  
Vol 8 (2) ◽  
pp. 001-009
Author(s):  
Masataka Yoshikawa ◽  
Ayano Miyamoto ◽  
Ryoichi Ikezawa ◽  
Hitomi Nakama ◽  
Ikuo Nishikawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Subcutaneous Injection ◽  
Immunosuppressive Agents ◽  
Systemic Administration ◽  
Nodule Formation ◽  
Dental Pulp Cells ◽  
Hard Tissue ◽  
Proliferation And Differentiation ◽  
Pulp Cells

Introduction: The purpose of this study was to assess the significant proliferation of dental pulp-derived stem cells in vitro from rats with the systemic administration of immunosuppressant in subcutis. There must be a sufficient number of stem cells for tooth regeneration. However, number of mesenchymal stem cells in the dental pulp tissue is a small. Then, the proliferation of stem cells must be accelerated for hard tissue formation. The subcutaneous injection of the immunosuppressant would enhance the hard tissue forming ability of dental pulp cells of rat. It was hypothesized in this study that differentiation of stem cells into blasts would be effectively promoted by suppression of the systemic immune response. Materials and methods: The dental pulp cells of rats with immunosuppressant injection subcutaneously were cultured with or without addition of the immunosuppressant in the medium containing dexamethasone for calcified nodule formation. Ca2+ by decalcification of calcified nodules were quantitatively analysed. Statistical comparisons between the quantities of Ca2+ were performed using two-way unrepeated ANOVA followed by post hoc analysis with Tukey-Kramer’s test. Differences of p < 0.01 were considered significant. Results: The proliferation and differentiation of stem cells among dental pulp cells was inhibited by the presence of immunosuppressive agents in the culture medium. However, stem cells obtained from rats after systemic administration of an immunosuppressive agent exhibited a high ability to form calcified nodules. Conclusions: To promote proliferation and differentiation of stem cells, systemic administration of an immunosuppressant to individuals prior to harvesting stem cells would be recommended.

Stem cells in dentistry – Review of literature

2013 ◽  
Vol 16 (1) ◽  
pp. 135-140 ◽  
Author(s):  
P. Dziubińska ◽  
M. Jaskólska ◽  
P. Przyborowska ◽  
Z. Adamiak
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Somatic Cells ◽  
Basic Knowledge ◽  
Dental Pulp Cells ◽  
Review Of Literature ◽  
Animal Tissues ◽  
Regenerative Dentistry ◽  
Pulp Cells ◽  
Made In

Abstract Stem cells have been successfully isolated from a variety of human and animal tissues, including dental pulp. This achievement marks progress in regenerative dentistry. This article reviews the latest improvements made in regenerative dental medicine with the involvement of stem cells. Although, various types of multipotent somatic cells can be applied in dentistry, two types of cells have been investigated in this review. Dental pulp cells are classified as: DPSCs, SCAPs and SHEDs.The third group includes two types of cell associated with the periodontium: PDL and DFPC. This review aims to systematize basic knowledge about cellular engineering in dentistry.

scholarly journals The Preparation and Characterization of Chitosan-Based Hydrogels Cross-Linked by Glyoxal

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2449
Author(s):  
Beata Kaczmarek-Szczepańska ◽  
Olha Mazur ◽  
Marta Michalska-Sionkowska ◽  
Krzysztof Łukowicz ◽  
Anna Maria Osyczka
Keyword(s):  
Thermal Stability ◽  
Tannic Acid ◽  
Dental Pulp ◽  
Blood Compatibility ◽  
Dental Pulp Cells ◽  
Preliminary Assessment ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

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