microRNA‐491‐5p regulates osteogenic differentiation of bone marrow stem cells in type 2 diabetes

Oral Diseases ◽  
2021 ◽  
Author(s):  
Lingxiao Wang ◽  
Chao Liang ◽  
Xiao Lin ◽  
Changying Liu ◽  
Jun Li
Keyword(s):  
Type 2 Diabetes ◽  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Bone Marrow Stem Cells

scholarly journals Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells

Biology ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 370
Author(s):  
Alessio Rochira ◽  
Luisa Siculella ◽  
Fabrizio Damiano ◽  
Andrea Palermo ◽  
Franco Ferrante ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Protein Quantification ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Osteogenic Medium ◽  
Alizarin Red ◽  
Matrix Mineralization

Bone regeneration is a complex process regulated by several factors that control overlapping biological processes, coordinating interactions among distinct cell populations. There is a great interest in identifying new strategies for inducing osteogenesis in a safe and efficient manner. Concentrated Growth Factor (CGF) is an autologous blood derived product obtained by centrifugation of venous blood following the procedure set on the Silfradent device. In this study the effects of CGF on osteogenic differentiation of human Bone Marrow Stem Cells (hBMSC) in vitro have been investigated; hBMSC were cultured with CGF or osteogenic medium, for 21 days. The osteogenic differentiation was evaluated measuring alkaline phosphatase (ALP) enzyme activity, matrix mineralization by alizarin red staining and through mRNA and protein quantification of osteogenic differentiation markers by Real-time PCR and Western blotting, respectively. The treatment with CGF stimulated ALP activity and promoted matrix mineralization compared to control and seems to be more effective than osteogenic medium. Also, hBMSC lost mesenchymal markers and showed other osteogenic features. Our study showed for the first time that CGF alone is able to induce osteogenic differentiation in hBMSC. The application of CGF on hBMSC osteoinduction might offer new clinical and biotechnological strategies in the tissue regeneration field.

The safety of bone-marrow derived mesenchymal stem cells in patients with type 2 diabetes mellitus

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S82 ◽  
Author(s):  
P. Adorable-Wagan ◽  
S. Bernal ◽  
D. Lavilles ◽  
M. De Vera
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Type 2 Diabetes Mellitus

Effect of Platelet Releasate on Osteogenic Differentiation of Human Mesenchymal Bone Marrow Stem Cells

2014 ◽  
Vol 156 (4) ◽  
pp. 560-565 ◽  
Author(s):  
S. M. Kosmacheva ◽  
N. N. Danilkovich ◽  
A. V. Shchepen’ ◽  
S. I. Ignatenko ◽  
M. P. Potapnev
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Platelet Releasate

Transplantation of Bone Marrow Mesenchymal Stem Cells for the Treatment of Type 2 Diabetes in a Macaque Model

2013 ◽  
Vol 198 (6) ◽  
pp. 414-427 ◽  
Author(s):  
Xing-hua Pan ◽  
Qiao-qiao Song ◽  
Jie-jie Dai ◽  
Xiang Yao ◽  
Jin-xiang Wang ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Macaque Model ◽  
Marrow Mesenchymal Stem Cells

scholarly journals LMCD1 promotes osteogenic differentiation of human bone marrow stem cells by regulating BMP signaling

2019 ◽  
Vol 10 (9) ◽  
Author(s):  
Bin Zhu ◽  
Feng Xue ◽  
Changqing Zhang ◽  
Guangyi Li
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Adipogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Bmp Signaling ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Homeostasis

Abstract Human bone marrow stem cells (BMSCs) are heterogeneous progenitor cells with two defining features, self-renew and multi-lineage differentiation. As one of the differentiation directions, osteogenesis is vital for bone homeostasis. A growing body of evidences show that ubiquitin-dependent protein degradation plays an essential role in the osteogenic differentiation of BMSCs. In this study, we found that LMCD1 was upregulated during osteogenic differentiation process of BMSCs by analyzing GSE80614. In vitro and in vivo functional studies confirmed that LMCD1 was critical to the osteogenic commitment of BMSCs. Compared to those of the controls, downregulation of LMCD1 significantly restrained osteogenic differentiation and enhanced adipogenic differentiation, while upregulation of LMCD1 increased the osteogenic differentiation and suppressed adipogenic differentiation. Mechanically, we found that LMCD1 could protect RUNX2 and Smad1 protein from Smurf1-induced ubiquitination degradation thereby regulating BMP signaling. In conclusion, our findings suggest that LMCD1 is a novel regulator of osteogenic differentiation and may be a potential therapeutic target for bone metabolism related diseases.

Identification of suitable culture condition for expansion and osteogenic differentiation of human bone marrow stem cells

Human Cell ◽  
2012 ◽  
Vol 25 (3) ◽  
pp. 69-77 ◽  
Author(s):  
Shiplu Roy Chowdhury ◽  
Min Hwei Ng ◽  
Norazril Shamsul Abu Hassan ◽  
Bin Saim Aminuddin ◽  
Bt Hj Idrus Ruszymah
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Culture Condition ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow

MiR-181d-5p regulates implant surface roughness-induced osteogenic differentiation of bone marrow stem cells

2021 ◽  
Vol 121 ◽  
pp. 111801
Author(s):  
Yanping Liu ◽  
Yixiang Wang ◽  
Xian Cheng ◽  
Yan Zheng ◽  
Mingyue Lyu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Surface Roughness ◽  
Osteogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Implant Surface

Effect of substrate stiffness on the osteogenic differentiation of bone marrow stem cells and bone-derived cells

2013 ◽  
Vol 37 (6) ◽  
pp. 608-616 ◽  
Author(s):  
Malgorzata Witkowska-Zimny ◽  
Katarzyna Walenko ◽  
Edyta Wrobel ◽  
Piotr Mrowka ◽  
Agnieszka Mikulska ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Substrate Stiffness
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