Effect of substrate stiffness on the osteogenic differentiation of bone marrow stem cells and bone-derived cells

2013 ◽  
Vol 37 (6) ◽  
pp. 608-616 ◽  
Author(s):  
Malgorzata Witkowska-Zimny ◽  
Katarzyna Walenko ◽  
Edyta Wrobel ◽  
Piotr Mrowka ◽  
Agnieszka Mikulska ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Substrate Stiffness

scholarly journals Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells

Biology ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 370
Author(s):  
Alessio Rochira ◽  
Luisa Siculella ◽  
Fabrizio Damiano ◽  
Andrea Palermo ◽  
Franco Ferrante ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Protein Quantification ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Osteogenic Medium ◽  
Alizarin Red ◽  
Matrix Mineralization

Bone regeneration is a complex process regulated by several factors that control overlapping biological processes, coordinating interactions among distinct cell populations. There is a great interest in identifying new strategies for inducing osteogenesis in a safe and efficient manner. Concentrated Growth Factor (CGF) is an autologous blood derived product obtained by centrifugation of venous blood following the procedure set on the Silfradent device. In this study the effects of CGF on osteogenic differentiation of human Bone Marrow Stem Cells (hBMSC) in vitro have been investigated; hBMSC were cultured with CGF or osteogenic medium, for 21 days. The osteogenic differentiation was evaluated measuring alkaline phosphatase (ALP) enzyme activity, matrix mineralization by alizarin red staining and through mRNA and protein quantification of osteogenic differentiation markers by Real-time PCR and Western blotting, respectively. The treatment with CGF stimulated ALP activity and promoted matrix mineralization compared to control and seems to be more effective than osteogenic medium. Also, hBMSC lost mesenchymal markers and showed other osteogenic features. Our study showed for the first time that CGF alone is able to induce osteogenic differentiation in hBMSC. The application of CGF on hBMSC osteoinduction might offer new clinical and biotechnological strategies in the tissue regeneration field.

Effect of Platelet Releasate on Osteogenic Differentiation of Human Mesenchymal Bone Marrow Stem Cells

2014 ◽  
Vol 156 (4) ◽  
pp. 560-565 ◽  
Author(s):  
S. M. Kosmacheva ◽  
N. N. Danilkovich ◽  
A. V. Shchepen’ ◽  
S. I. Ignatenko ◽  
M. P. Potapnev
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Platelet Releasate

scholarly journals LMCD1 promotes osteogenic differentiation of human bone marrow stem cells by regulating BMP signaling

2019 ◽  
Vol 10 (9) ◽  
Author(s):  
Bin Zhu ◽  
Feng Xue ◽  
Changqing Zhang ◽  
Guangyi Li
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Adipogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Bmp Signaling ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Homeostasis

Abstract Human bone marrow stem cells (BMSCs) are heterogeneous progenitor cells with two defining features, self-renew and multi-lineage differentiation. As one of the differentiation directions, osteogenesis is vital for bone homeostasis. A growing body of evidences show that ubiquitin-dependent protein degradation plays an essential role in the osteogenic differentiation of BMSCs. In this study, we found that LMCD1 was upregulated during osteogenic differentiation process of BMSCs by analyzing GSE80614. In vitro and in vivo functional studies confirmed that LMCD1 was critical to the osteogenic commitment of BMSCs. Compared to those of the controls, downregulation of LMCD1 significantly restrained osteogenic differentiation and enhanced adipogenic differentiation, while upregulation of LMCD1 increased the osteogenic differentiation and suppressed adipogenic differentiation. Mechanically, we found that LMCD1 could protect RUNX2 and Smad1 protein from Smurf1-induced ubiquitination degradation thereby regulating BMP signaling. In conclusion, our findings suggest that LMCD1 is a novel regulator of osteogenic differentiation and may be a potential therapeutic target for bone metabolism related diseases.

Identification of suitable culture condition for expansion and osteogenic differentiation of human bone marrow stem cells

Human Cell ◽  
2012 ◽  
Vol 25 (3) ◽  
pp. 69-77 ◽  
Author(s):  
Shiplu Roy Chowdhury ◽  
Min Hwei Ng ◽  
Norazril Shamsul Abu Hassan ◽  
Bin Saim Aminuddin ◽  
Bt Hj Idrus Ruszymah
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Culture Condition ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow

MiR-181d-5p regulates implant surface roughness-induced osteogenic differentiation of bone marrow stem cells

2021 ◽  
Vol 121 ◽  
pp. 111801
Author(s):  
Yanping Liu ◽  
Yixiang Wang ◽  
Xian Cheng ◽  
Yan Zheng ◽  
Mingyue Lyu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Surface Roughness ◽  
Osteogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Implant Surface

scholarly journals Shared Genetic and Epigenetic Mechanisms between the Osteogenic Differentiation of Dental Pulp Stem Cells and Bone Marrow Stem Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-25
Author(s):  
Sebastian Gaus ◽  
Hanluo Li ◽  
Simin Li ◽  
Qian Wang ◽  
Tina Kottek ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Signaling Pathways ◽  
Expression Pattern ◽  
Dental Pulp ◽  
Target Genes ◽  
Bone Marrow Stem Cells ◽  
Epigenetic Mechanisms ◽  
Shared Genetic

Objective. To identify the shared genetic and epigenetic mechanisms between the osteogenic differentiation of dental pulp stem cells (DPSC) and bone marrow stem cells (BMSC). Materials and Methods. The profiling datasets of miRNA expression in the osteogenic differentiation of mesenchymal stem cells from the dental pulp (DPSC) and bone marrow (BMSC) were searched in the Gene Expression Omnibus (GEO) database. The differential expression analysis was performed to identify differentially expressed miRNAs (DEmiRNAs) dysregulated in DPSC and BMSC osteodifferentiation. The target genes of the DEmiRNAs that were dysregulated in DPSC and BMSC osteodifferentiation were identified, followed by the identification of the signaling pathways and biological processes (BPs) of these target genes. Accordingly, the DEmiRNA-transcription factor (TFs) network and the DEmiRNAs-small molecular drug network involved in the DPSC and BMSC osteodifferentiation were constructed. Results. 16 dysregulated DEmiRNAs were found to be overlapped in the DPSC and BMSC osteodifferentiation, including 8 DEmiRNAs with a common expression pattern (8 upregulated DEmiRNAs (miR-101-3p, miR-143-3p, miR-145-3p/5p, miR-19a-3p, miR-34c-5p, miR-3607-3p, miR-378e, miR-671-3p, and miR-671-5p) and 1 downregulated DEmiRNA (miR-671-3p/5p)), as well as 8 DEmiRNAs with a different expression pattern (i.e., miR-1273g-3p, miR-146a-5p, miR-146b-5p, miR-337-3p, miR-382-3p, miR-4508, miR-4516, and miR-6087). Several signaling pathways (TNF, mTOR, Hippo, neutrophin, and pathways regulating pluripotency of stem cells), transcription factors (RUNX1, FOXA1, HIF1A, and MYC), and small molecule drugs (curcumin, docosahexaenoic acid (DHA), vitamin D3, arsenic trioxide, 5-fluorouracil (5-FU), and naringin) were identified as common regulators of both the DPSC and BMSC osteodifferentiation. Conclusion. Common genetic and epigenetic mechanisms are involved in the osteodifferentiation of DPSCs and BMSCs.

scholarly journals The influence of age and osteoporosis on bone marrow stem cells from rats

2018 ◽  
Vol 7 (4) ◽  
pp. 289-297 ◽  
Author(s):  
A. Sanghani-Kerai ◽  
L. Osagie-Clouard ◽  
G. Blunn ◽  
M. Coathup
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Adipogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Female Rats ◽  
Alizarin Red ◽  
Young Rat ◽  
Cd Marker

Objectives This study aimed to assess the effect of age and osteoporosis on the proliferative and differentiating capacity of bone-marrow-derived mesenchymal stem cells (MSCs) in female rats. We also discuss the role of these factors on expression and migration of cells along the C-X-C chemokine receptor type 4 (CXCR-4) / stromal derived factor 1 (SDF-1) axis. Methods Mesenchymal stem cells were harvested from the femora of young, adult, and osteopenic Wistar rats. Cluster of differentiation (CD) marker and CXCR-4 expression was measured using flow cytometry. Cellular proliferation was measured using Alamar Blue, osteogenic differentiation was measured using alkaline phosphatase expression and alizarin red production, and adipogenic differentiation was measured using Oil red O. Cells were incubated in Boyden chambers to quantify their migration towards SDF-1. Data was analyzed using a Student’s t-test, where p-values < 0.05 were considered significant. Results CD marker expression and proliferation of the MSCs from the three groups was not significantly different. The young MSCs demonstrated significantly increased differentiation into bone and fat and superior migration towards SDF-1. The migration of SDF-1 doubled with young rats compared with the adult rats (p = 0.023) and it was four times higher when compared with cells isolated from ovariectomized (OVX) osteopenic rats (p = 0.013). Conclusion Young rat MSCs are significantly more responsive to osteogenic differentiation, and, contrary to other studies, also demonstrated increased adipogenic differentiation compared with cells from adult and ostopenic rats. Young-rat-derived cells also showed superior migration towards SDF-1 compared with MSCs from OVX and adult control rats. Cite this article: A. Sanghani-Kerai, L. Osagie-Clouard, G. Blunn, M. Coathup. The influence of age and osteoporosis on bone marrow stem cells from rats. Bone Joint Res 2018;7:289–297. DOI: 10.1302/2046-3758.74.BJR-2017-0302.R1.

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