Osmotic stress represses root growth by modulating the transcriptional regulation of PIN‐FORMED3

2021 ◽  
Author(s):  
Ting‐Ting Yuan ◽  
Zhi‐Xin Xiang ◽  
Wen Li ◽  
Xiang Gao ◽  
Ying‐Tang Lu
Keyword(s):  
Transcriptional Regulation ◽  
Osmotic Stress ◽  
Root Growth

scholarly journals The Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 Gene Is Involved in Anisotropic Root Growth during Osmotic Stress Adaptation

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 236
Author(s):  
María Belén Cuadrado-Pedetti ◽  
Inés Rauschert ◽  
María Martha Sainz ◽  
Vítor Amorim-Silva ◽  
Miguel Angel Botella ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Root Growth ◽  
Cell Walls ◽  
Primary Root ◽  
Stress Adaptation ◽  
Elongation Zone ◽  
Cortical Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Mean

Mutations in the Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 (TTL1) gene cause reduced tolerance to osmotic stress evidenced by an arrest in root growth and root swelling, which makes it an interesting model to explore how root growth is controlled under stress conditions. We found that osmotic stress reduced the growth rate of the primary root by inhibiting the cell elongation in the elongation zone followed by a reduction in the number of cortical cells in the proximal meristem. We then studied the stiffness of epidermal cell walls in the root elongation zone of ttl1 mutants under osmotic stress using atomic force microscopy. In plants grown in control conditions, the mean apparent elastic modulus was 448% higher for live Col-0 cell walls than for ttl1 (88.1 ± 2.8 vs. 16.08 ± 6.9 kPa). Seven days of osmotic stress caused an increase in the stiffness in the cell wall of the cells from the elongation zone of 87% and 84% for Col-0 and ttl1, respectively. These findings suggest that TTL1 may play a role controlling cell expansion orientation during root growth, necessary for osmotic stress adaptation.

scholarly journals A MYB-related Transcription Factor from Sheepgrass, LcMYB2, Promotes Seed Germination and Root Growth under Drought Stress

2019 ◽  
Author(s):  
Pincang Zhao ◽  
Shenglin Hou ◽  
xiufang guo ◽  
Junting Jia ◽  
Weiguang Yang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Root Growth ◽  
Genetic Manipulation ◽  
Marker Genes ◽  
Plant Responses ◽  
Adverse Conditions ◽  
Related Transcription Factor

Abstract Background Drought is one of the most serious factors limiting plant growth and production. Sheepgrass can adapt well to various adverse conditions, including drought. However, during germination, sheepgrass young seedlings are sensitive to these adverse conditions. Therefore, the adaptability of seedlings is very important for plant survival, especially in plants that inhabit grasslands or the construction of artificial grassland. Results In this study, we found a sheepgrass MYB-related transcription factor, LcMYB2 that is up-regulated by drought stress and returns to a basal level after rewatering. The expression of LcMYB2 was mainly induced by osmotic stress and was localized to the nucleus. Furthermore, we demonstrate that LcMYB2 promoted seed germination and root growth under drought and ABA treatments. Additionally, we confirmed that LcMYB2 can regulate LcDREB2 expression in sheepgrass by binding to its promoter, and it activates the expression of the osmotic stress marker genes AtDREB2A, AtLEA14 and AtP5CS1 by directly binding to their promoters in transgenic Arabidopsis. Conclusions Based on these results, we propose that LcMYB2 improves plant drought stress tolerance by increasing the accumulation of osmoprotectants and promoting root growth. Therefore, LcMYB2 plays pivotal roles in plant responses to drought stress and is an important candidate for genetic manipulation to create drought-resistant crops, especially during seed germination.

Germination Ecology of Milkweedvine (Morrenia odorata)

Weed Science ◽  
1984 ◽  
Vol 32 (6) ◽  
pp. 781-785 ◽  
Author(s):  
Megh Singh ◽  
Nagi Reddy Achhireddy
Keyword(s):  
Seed Germination ◽  
Osmotic Stress ◽  
Root Growth ◽  
Seedling Growth ◽  
Shoot Growth ◽  
Seedling Emergence ◽  
Germination Percentage ◽  
Planting Depth ◽  
Germination Ecology ◽  
Optimum Ph

The germination of milkweedvine (Morrenia odorataLindl. ♯3MONOD) seed at 20 or 25 C was unaffected by a 12-h photoperiod. The 12-h photoperiod, however, decreased germination by 50% at 15 C. No germination occurred at 35 C regardless of photoperiod. By alternating 35 C for 12 h with 20 C for 12 h, the germination percentage was 57%. Seedling growth was maximum at alternating temperatures of 30/20 C. Optimum pH for germination and seedling growth was 7 and germination did not occur at pH levels below 6. Seed germination declined steadily at osmotic stress below −0.12 MPa; no germination occurred at −0.5 MPa. Seedling growth was not influenced by osmotic stress down to −0.18 MPa. Germination percentages of seeds kept under aerated water and nonaerated water were similar, but the seedling growth was greater in aerated water. Seedling emergence was maximum from depths of 0.5 to 2.5 cm, but no seedling emerged from 0 or 10 cm. Planting depth was negatively correlated (r = −0.7) with shoot growth but positively correlated (r = +0.98) to root growth.

scholarly journals Maize seedling performance as a potential index for drought tolerance

2018 ◽  
Vol 70 (1) ◽  
pp. 167-177
Author(s):  
Natalija Kravic ◽  
Sukalovic Hadzi-Taskovic ◽  
Vojka Babic ◽  
Jelena Srdic ◽  
Jelena Mesarovic ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Osmotic Stress ◽  
Root Growth ◽  
Grain Yield ◽  
Maize Seedling ◽  
Proline Content ◽  
Seedling Root ◽  
Early Seedling ◽  
Positive Correlations ◽  
Adult Plants

Twenty-six maize landraces were tested in order to evaluate maize seedling performance as an index for drought tolerance in adult plants. Samples were subjected to polyethylene glycol-induced osmotic stress at the early seedling stage. Grain yield was obtained in field experiments under well-watered (OC) and a combination of drought and high plant density (HD) conditions. Osmotic stress caused a reduction in seedling growth (length, fresh and dry weight), and increase in the shoot and in particular the root proline contents in the majority of landraces, and variations in root peroxidase (POD) activity. Genotypes displaying more pronounced root growth reduction and higher proline contents exhibited decreased POD activity under osmotic stress. Direct positive correlations between the proline content and growth inhibition, and between the proline and soluble protein content were established. Correlations between the changes in POD activity and growth parameters were significant and positive, and significant but negative with the changes in the proline content. In the field, water stress led to a reduction in grain yield in all of the tested landraces. Correlations between grain yield from both experimental sets (OC and HD) and osmotic-induced changes in seedling root growth were negative, which was opposite to the highly significant and positive correlations between the changes in the seedling root proline content and yield. Also, genotypes with the highest seedling root proline content increase under osmotic stress, exhibited the highest stress tolerance index (STI) based on grain yield achieved under both field conditions. Our results indicate that lower changes in POD activity and especially an increased proline content after exposure to osmotic stress during the early seedling stage could be considered as useful indices to facilitate selection efficiency for drought tolerance in adult plants.

scholarly journals A MYB-related Transcription Factor from Sheepgrass, LcMYB2, Promotes Seed Germination and Root Growth under Drought Stress

2019 ◽  
Author(s):  
xiufang guo ◽  
Pincang Zhao ◽  
Shenglin Hou ◽  
Junting Jia ◽  
Weiguang Yang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Root Growth ◽  
Genetic Manipulation ◽  
Marker Genes ◽  
Plant Responses ◽  
Adverse Conditions ◽  
Related Transcription Factor

Abstract Background Drought is one of the most serious factors limiting plant growth and production. Sheepgrass can adapt well to various adverse conditions, including drought. However, during germination, sheepgrass young seedlings are sensitive to these adverse conditions. Therefore, the adaptability of seedlings is very important for plant survival, especially in plants that inhabit grasslands or the construction of artificial grassland. Results In this study, we found a sheepgrass MYB-related transcription factor, LcMYB2 that is up-regulated by drought stress and returns to a basal level after rewatering. The expression of LcMYB2 was mainly induced by osmotic stress and was localized to the nucleus. Furthermore, we demonstrate that LcMYB2 promoted seed germination and root growth under drought and ABA treatments. Additionally, we confirmed that LcMYB2 can regulate LcDREB2 expression in sheepgrass by binding to its promoter, and it activates the expression of the osmotic stress marker genes AtDREB2A, AtLEA14 and AtP5CS1 by directly binding to their promoters in transgenic Arabidopsis. Conclusions Based on these results, we propose that LcMYB2 improves plant drought stress tolerance by increasing the accumulation of osmoprotectants and promoting root growth. Therefore, LcMYB2 plays pivotal roles in plant responses to drought stress and is an important candidate for genetic manipulation to create drought-resistant crops, especially during seed germination.

scholarly journals The role of osmotic adjustment, cell production and sugar accumulation for the root growth under the osmotic stress condition.

Root Research ◽  
2007 ◽  
Vol 16 (2) ◽  
pp. 47-58
Author(s):  
Atsushi Ogawa ◽  
Choji Kawashima ◽  
Kinji Kitamichi ◽  
Kyoko Toyofuku ◽  
Akira Yamauchi
Keyword(s):  
Osmotic Stress ◽  
Root Growth ◽  
Osmotic Adjustment ◽  
Stress Condition ◽  
Sugar Accumulation ◽  
Cell Production

Boron delays dehydration and stimulates root growth in Eucalyptus urophylla (Blake, S.T.) under osmotic stress

2014 ◽  
Vol 384 (1-2) ◽  
pp. 185-199 ◽  
Author(s):  
Bárbara Elias Reis Hodecker ◽  
Nairam Félix De Barros ◽  
Ivo Ribeiro Da Silva ◽  
Valdir Diola ◽  
Jorge Eduardo Souza Sarkis ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Root Growth ◽  
Eucalyptus Urophylla
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