scholarly journals Novel chemicals engender myriad invasion mechanisms

2021 ◽  
Vol 232 (3) ◽  
pp. 1184-1200
Author(s):  
Inderjit ◽  
Daniel Simberloff ◽  
Harleen Kaur ◽  
Susan Kalisz ◽  
T. Martijn Bezemer
Keyword(s):  
Invasion Mechanisms

Characterisation of NcGRA7 and NcSAG4 proteins: Immunolocalisation and their role in the host cell invasion by Neospora caninum tachyzoites

2010 ◽  
Vol 55 (4) ◽  
Author(s):  
Adriana Aguado-Martínez ◽  
Gema Álvarez-García ◽  
Gereon Schares ◽  
Verónica Risco-Castillo ◽  
Aurora Fernández-García ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Host Cell ◽  
Cell Invasion ◽  
Neospora Caninum ◽  
Parasitophorous Vacuole ◽  
Membrane Surface ◽  
Chronic Phase ◽  
Host Cell Invasion ◽  
Invasion Mechanisms ◽  
The Matrix

AbstractNeospora caninum negatively impacts bovine reproductive performance around the world. Addressing this problem requires a greater understanding of the parasite’s molecular biology. In this study, monoclonal antibodies against recombinant proteins were successfully developed and employed to characterise two different proteins of N. caninum: the acute phase-associated NcGRA7 and the chronic phase-associated NcSAG4. Immunofluorescence with the anti-rNcGRA7 monoclonal antibody suggested that NcGRA7 trafficks from tachyzoite dense granules to the matrix of the parasitophorous vacuole and parasite’s surroundings. Furthermore, NcGRA7 is also expressed in the bradyzoite stage and localised on the matrix of bradyzoite-positive vacuoles. NcGRA7 appears to be partially involved in the tachyzoite-invasion mechanisms, as an anti-rNcGRA7 monoclonal antibody partially inhibited in vitro tachyzoite-invasion. A monoclonal antibody specific for NcSAG4 confirmed this protein’s bradyzoitespecific expression both by western blot and immunofluorescence. However, some bradyzoite-positive vacuoles only weakly expressed NcSAG4, if it was expressed at all. The specificity of the anti-rNcSAG4 monoclonal antibody was confirmed by the recognition of the NcSAG4 in the membrane surface of Nc-1SAG4c transgenic tachyzoites, which constitutively expresses NcSAG4. Blocking NcSAG4 of Nc-1SAG4c tachyzoites with the monoclonal antibody did not affect host cell invasion. However, its implication on the host cell adhesion or host immune evasion should not be discarded.

Invasion Mechanisms

1995 ◽  
pp. 307-322 ◽  
Author(s):  
Jean François Dubremetz ◽  
James H. McKerrow
Keyword(s):  
Invasion Mechanisms

Investigation of Tumour-Invasion Mechanisms

1987 ◽  
pp. 359-370
Author(s):  
M. Mareel ◽  
F. Van Roy ◽  
L. Messiaen ◽  
M. Bracke ◽  
E. Boghaert ◽  
...  
Keyword(s):  
Tumour Invasion ◽  
Invasion Mechanisms

scholarly journals Microbial’s Effects on Invasive Grass’ Response to Water and Nutrient Stress

2020 ◽  
Author(s):  
Bo Zhang ◽  
Yingdan Yuan ◽  
Karolina M. Pajerowska-Mukhtar ◽  
Michelle Afkhami ◽  
Alan Hastings ◽  
...  
Keyword(s):  
Large Scale ◽  
Interactive Effect ◽  
Invasion Biology ◽  
Imperata Cylindrica ◽  
Invasion Success ◽  
Nutrient Concentrations ◽  
Sequencing Analysis ◽  
Drought Treatment ◽  
Plant Microbe Interaction ◽  
Invasion Mechanisms

Abstract Background Soil microbiomes play important roles in invasion biology, yet it is often treated as a ‘black box’ in modeling or large-scale field studies. Hence, investigating the change of association between invasive vegetation and soil microbes under changing environmental conditions, and exploring the genetic functions of associated microbiomes will provide a deeper understanding of invasion mechanisms. We performed a microcosm experiment with cogongrass (Imperata cylindrica (L.) P. Beauv.), which is one of the 100 worst invasive plants in the world. We combined rigorous sequencing analysis, including 16S rRNA, ITS, and shotgun metagenome sequencing, for the first time, to investigate the interactive effect of change in soil water and nutrient concentrations on microbiomes diversity, composition and genetic functions under invasion. Results We found that experimental drought has a stronger effect on the bacterial community than the fungal community. We discovered an enrichment of microbial groups, including Proteobacteria, Actinobacteria, Bacteroidetes and Chloroflexi under drought treatment, could likely contribute to invasion success. Further, we showed a striking trend of induction of cell wall, membrane and desiccation-related genes in drought treatment and a marked downregulation in regular treatment, which could create a more hydrated microenvironment, facilitating biofilm formation and better protection from desiccation.Conclusions Our work contributes to highlighting the associated microbial communities may have a potential long-term impact on increasing cogongrass drought resistance, ultimately, future invasion might be severe due to the plant-microbe interaction. These findings are important because current modeling practice, lacking comprehensive consideration of the plant-microbe interaction, could lead to a significant underestimate of predictions of future invasion patterns.

scholarly journals Role of KCa3.1 Channels in Modulating Ca2+ Oscillations during Glioblastoma Cell Migration and Invasion

2018 ◽  
Vol 19 (10) ◽  
pp. 2970 ◽  
Author(s):  
Luigi Catacuzzeno ◽  
Fabio Franciolini
Keyword(s):  
Cell Migration ◽  
Theoretical Model ◽  
Migration And Invasion ◽  
Channel Activity ◽  
Cell Migration And Invasion ◽  
Channel Modulation ◽  
Invasion Mechanisms ◽  
Migratory Cycle ◽  
Channel Dependent

Cell migration and invasion in glioblastoma (GBM), the most lethal form of primary brain tumors, are critically dependent on Ca2+ signaling. Increases of [Ca2+]i in GBM cells often result from Ca2+ release from the endoplasmic reticulum (ER), promoted by a variety of agents present in the tumor microenvironment and able to activate the phospholipase C/inositol 1,4,5-trisphosphate PLC/IP3 pathway. The Ca2+ signaling is further strengthened by the Ca2+ influx from the extracellular space through Ca2+ release-activated Ca2+ (CRAC) currents sustained by Orai/STIM channels, meant to replenish the partially depleted ER. Notably, the elevated cytosolic [Ca2+]i activates the intermediate conductance Ca2+-activated K (KCa3.1) channels highly expressed in the plasma membrane of GBM cells, and the resulting K+ efflux hyperpolarizes the cell membrane. This translates to an enhancement of Ca2+ entry through Orai/STIM channels as a result of the increased electromotive (driving) force on Ca2+ influx, ending with the establishment of a recurrent cycle reinforcing the Ca2+ signal. Ca2+ signaling in migrating GBM cells often emerges in the form of intracellular Ca2+ oscillations, instrumental to promote key processes in the migratory cycle. This has suggested that KCa3.1 channels may promote GBM cell migration by inducing or modulating the shape of Ca2+ oscillations. In accordance, we recently built a theoretical model of Ca2+ oscillations incorporating the KCa3.1 channel-dependent dynamics of the membrane potential, and found that the KCa3.1 channel activity could significantly affect the IP3 driven Ca2+ oscillations. Here we review our new theoretical model of Ca2+ oscillations in GBM, upgraded in the light of better knowledge of the KCa3.1 channel kinetics and Ca2+ sensitivity, the dynamics of the Orai/STIM channel modulation, the migration and invasion mechanisms of GBM cells, and their regulation by Ca2+ signals.

Research on Spartina alterniflora using molecular biological techniques: an overview

2020 ◽  
Vol 71 (12) ◽  
pp. 1564
Author(s):  
Lu Xia ◽  
Wen Yang ◽  
Qifang Geng ◽  
Nasreen Jeelani ◽  
Shuqing An
Keyword(s):  
Spartina Alterniflora ◽  
Double Helix ◽  
Biological Macromolecules ◽  
Invasion Mechanisms ◽  
Global Issue ◽  
Invasive Organisms ◽  
Double Helix Structure ◽  
The Individual ◽  
And Function ◽  
Molecular Biological Techniques

Biological invasion is a global issue. Since the double helix structure of DNA molecule was discovered in 1953, more scientific studies have focused on the structure and function of biological macromolecules in invasive organisms using molecular biology techniques, which has contributed to our understanding of their competitive advantages and invasion mechanisms. As a coastal invasive species, there has been considerable interest in Spartina alterniflora. Here we summarise previous studies investigating S. alterniflora using molecular biological techniques from the individual, population and application perspectives. We hope this article will be helpful in future studies on and in the management and utilisation of S. alterniflora in wetlands.

scholarly journals Invasion mechanisms of Gram-positive pathogenic cocci

2007 ◽  
Vol 98 (09) ◽  
pp. 488-496 ◽  
Author(s):  
Patric Nitsche-Schmitz ◽  
Manfred Rohde ◽  
Gursharan Chhatwal
Keyword(s):  
Surface Proteins ◽  
Host Cells ◽  
Human Pathogens ◽  
Gram Positive ◽  
Bacterial Surface ◽  
Surface Receptors ◽  
Major Threat ◽  
Invasion Mechanisms ◽  
Invasive Infections ◽  
Gram Positive Cocci

SummaryGram-positive cocci are important human pathogens. Streptococci and staphylococci in particular are a major threat to human health,since they cause a variety of serious invasive infections. Their invasion into normally sterile sites of the host depends on elaborated bacterial mechanisms that involve adhesion to the host tissue, its degradation, internalisation by host cells, and passage through epithelia and endothelia. Interactions of bacterial surface proteins with proteins of the host’s extracellular matrix as well as with cell surface receptors are crucial factors in these processes, and some of the key mechanisms are similar in many pathogenic Gram-positive cocci.Therapies that interfere with these mechanisms may become efficient alternatives to today’s antibiotic treatments.

scholarly journals Invasive Seaweeds in the Iberian Peninsula: A Contribution for Food Supply

Marine Drugs ◽  
2020 ◽  
Vol 18 (11) ◽  
pp. 560
Author(s):  
Diana Pacheco ◽  
Glacio Souza Araújo ◽  
João Cotas ◽  
Rui Gaspar ◽  
João M. Neto ◽  
...  
Keyword(s):  
Iberian Peninsula ◽  
Food Industry ◽  
Algal Species ◽  
Industrial Applications ◽  
Indigenous Species ◽  
Invasion Mechanisms ◽  
Daily Food ◽  
Research Findings ◽  
Non Indigenous Species ◽  
Nutraceutical Properties

The introduction of exotic organisms in marine ecosystems can lead to economic and ecological losses. Globally, seaweeds represent a significant part of these non-indigenous species (NIS), with 407 introduced algal species. Furthermore, the presence of NIS seaweeds has been reported as a major concern worldwide since the patterns of their potential invasion mechanisms and vectors are not yet fully understood. Currently, in the Iberian Peninsula, around 50 NIS seaweeds have been recorded. Some of these are also considered invasive due to their overgrowth characteristic and competition with other species. However, invasive seaweeds are suitable for industrial applications due to their high feedstock. Hence, seaweeds’ historical use in daily food diet, allied to research findings, showed that macroalgae are a source of nutrients and bioactive compounds with nutraceutical properties. The main goal of this review is to evaluate the records of NIS seaweeds in the Iberian Peninsula and critically analyze the potential of invasive seaweeds application in the food industry.

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