Research on Spartina alterniflora using molecular biological techniques: an overview

2020 ◽  
Vol 71 (12) ◽  
pp. 1564
Author(s):  
Lu Xia ◽  
Wen Yang ◽  
Qifang Geng ◽  
Nasreen Jeelani ◽  
Shuqing An
Keyword(s):  
Spartina Alterniflora ◽  
Double Helix ◽  
Biological Macromolecules ◽  
Invasion Mechanisms ◽  
Global Issue ◽  
Invasive Organisms ◽  
Double Helix Structure ◽  
The Individual ◽  
And Function ◽  
Molecular Biological Techniques

Biological invasion is a global issue. Since the double helix structure of DNA molecule was discovered in 1953, more scientific studies have focused on the structure and function of biological macromolecules in invasive organisms using molecular biology techniques, which has contributed to our understanding of their competitive advantages and invasion mechanisms. As a coastal invasive species, there has been considerable interest in Spartina alterniflora. Here we summarise previous studies investigating S. alterniflora using molecular biological techniques from the individual, population and application perspectives. We hope this article will be helpful in future studies on and in the management and utilisation of S. alterniflora in wetlands.

Depletion and Reconstitution of Active E. Coli Ribosomes

1975 ◽  
Vol 33 ◽  
pp. 640-641
Author(s):  
M. Boublik ◽  
W. Hellmann ◽  
F. Jenkins
Keyword(s):  
Protein Biosynthesis ◽  
Large Subunit ◽  
High Resolution Electron Microscopy ◽  
Small Subunit ◽  
Structure And Function ◽  
Biologically Active ◽  
Biological Macromolecules ◽  
E Coli ◽  
Resolution Electron ◽  
And Function

Correlations between structure and function of biological macromolecules have been studied intensively for many years, mostly by indirect methods. High resolution electron microscopy is a unique tool which can provide such information directly by comparing the conformation of biopolymers in their biologically active and inactive state. We have correlated the structure and function of ribosomes, ribonucleoprotein particles which are the site of protein biosynthesis. 70S E. coli ribosomes, used in this experiment, are composed of two subunits - large (50S) and small (30S). The large subunit consists of 34 proteins and two different ribonucleic acid molecules. The small subunit contains 21 proteins and one RNA molecule. All proteins (with the exception of L7 and L12) are present in one copy per ribosome.This study deals with the changes in the fine structure of E. coli ribosomes depleted of proteins L7 and L12. These proteins are unique in many aspects.

Narrow is the Gate to Utopia

Moreana ◽  
2012 ◽  
Vol 49 (Number 187- (1-2) ◽  
pp. 207-226
Author(s):  
Marie-Claire Phélippeau
Keyword(s):  
Social Construction ◽  
Free Will ◽  
Religious Toleration ◽  
Individual Liberty ◽  
The Reformation ◽  
The Individual ◽  
And Function

This study examines the notions of pleasure, individual liberty and consensus in Thomas More’s Utopia. The paradox inherent in Utopia, written before the Reformation, is especially visible in the affirmation of religious toleration coexisting with the need for a strict supervision of the citizens. The dream of an ideal republic is based on a Pauline vision of man which defines the individual mainly as a sinner. Consequently, it is the duty of the republic’s rulers to guide the citizens and establish a consensus. This study tries to determine the part left to the individual’s free will and examines the nature and function of the structures that are supposed to ensure the happiness of each one and of the whole community. The notion of moral hierarchy is asserted as the linchpin of the Utopian social construction.

DNA Inspirited Rational Construction of Nonconventional Luminophores with Efficient and Color-Tunable Afterglow

2020 ◽  
Author(s):  
Yunzhong Wang ◽  
Saixing Tang ◽  
Yating Wen ◽  
Shuyuan Zheng ◽  
Bing Yang ◽  
...  
Keyword(s):  
Rational Design ◽  
Room Temperature ◽  
Double Helix ◽  
Mechanical Grinding ◽  
Room Temperature Phosphorescence ◽  
Color Tunable ◽  
Potential Applications ◽  
Rational Construction ◽  
Double Helix Structure ◽  
Made In

<div>Persistent room-temperature phosphorescence (p-RTP) from pure organics is attractive </div><div>due to its fundamental importance and potential applications in molecular imaging, </div><div>sensing, encryption, anticounterfeiting, etc.1-4 Recently, efforts have been also made in </div><div>obtaining color-tunable p-RTP in aromatic phosphors5 and nonconjugated polymers6,7. </div><div>The origin of color-tunable p-RTP and the rational design of such luminogens, </div><div>particularly those with explicit structure and molecular packing, remain challenging. </div><div>Noteworthily, nonconventional luminophores without significant conjugations generally </div><div>possess excitation-dependent photoluminescence (PL) because of the coexistence of </div><div>diverse clustered chromophores6,8, which strongly implicates the possibility to achieve </div><div>color-tunable p-RTP from their molecular crystals assisted by effective intermolecular </div><div>interactions. Here, inspirited by the highly stable double-helix structure and multiple </div><div>hydrogen bonds in DNA, we reported a series of nonconventional luminophores based on </div><div>hydantoin (HA), which demonstrate excitation-dependent PL and color-tunable p-RTP </div><div>from sky-blue to yellowish-green, accompanying unprecedentedly high PL and p-RTP </div><div>efficiencies of up to 87.5% and 21.8%, respectively. Meanwhile, the p-RTP emissions are </div><div>resistant to vigorous mechanical grinding, with lifetimes of up to 1.74 s. Such robust, </div><div>color-tunable and highly efficient p-RTP render the luminophores promising for varying </div><div>applications. These findings provide mechanism insights into the origin of color-tunable </div><div>p-RTP, and surely advance the exploitation of efficient nonconventional luminophores.</div>

scholarly journals Adolescent and adult laryngotracheal stenosis: a review

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Gerhard Johan Klopper ◽  
Oladele Vincent Adeniyi ◽  
Kate Stephenson
Keyword(s):  
Intensive Care ◽  
Past Century ◽  
Main Body ◽  
Laryngotracheal Stenosis ◽  
Airway Protection ◽  
Interventional Pulmonology ◽  
The Past ◽  
Airway Emergency ◽  
The Individual ◽  
And Function

Abstract Background The larynx has multiple composite functions which include phonation, airway protection, and sensory control of respiration. Stenosis of the larynx and trachea were first recorded by O’Dwyer in 1885 and by Colles in 1886, respectively. Initially, the aetiology of laryngotracheal stenosis was predominantly infective. Currently, the leading cause is iatrogenic injury to the laryngotracheal complex secondary to prolonged ventilation in an intensive care unit. Main body Laryngotracheal stenosis is a complex and diverse disease. It poses a major challenge to the surgeon and can present as an airway emergency. Management typically demands the combined involvement of various disciplines including otorhinolaryngology, cardiothoracic surgery, anaesthesiology, interventional pulmonology, and radiology. Both the disease and its management can impact upon respiration, voice, and swallowing. The incidence of iatrogenic laryngotracheal stenosis has reflected the evolution of airway and intensive care whilst airway surgery has advanced concurrently over the past century. Correction of laryngotracheal stenosis requires expansion of the airway lumen; this is achieved by either endoscopic or open surgery. We review the relevant basic science, aetiopathogenesis, diagnosis, management, and treatment outcomes of LTS. Conclusion The choice of surgical procedure in the management of laryngotracheal stenosis is often dictated by the individual anatomy and function of the larynx and trachea, together with patient factors and available facilities. Regardless of how the surgeon chooses to approach these lesions, prevention of iatrogenic laryngotracheal damage remains of primary importance.

scholarly journals Continuous-Flow Photocatalytic Microfluidic-Reactor for the Treatment of Aqueous Contaminants, Simplicity, and Complexity: A Mini-Review

Symmetry ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1325
Author(s):  
Zhongwei Gao ◽  
Changqing Pan ◽  
Chang-Ho Choi ◽  
Chih-Hung Chang
Keyword(s):  
Continuous Flow ◽  
Lower Energy ◽  
High Efficiency ◽  
Diffusion Length ◽  
Physical Adsorption ◽  
Chemical Oxidation ◽  
Microfluidic Reactor ◽  
Global Issue ◽  
The Individual ◽  
Aqueous Contaminants

Water pollution is a growing global issue; there are many approaches to treating wastewater, including chemical coagulation, physical adsorption, and chemical oxidation. The photocatalysis process has provided a solution for removing pollutants from wastewater, where the pair of the photoelectron and hole works through an asymmetric way to degrade the contaminants under UV irradiation. This method offers an alternative route for treating the pollutant with a lower energy cost, high efficiency, and fewer byproducts. A continuous-flow microfluidic reactor has a channel size from tens to thousands of micrometers, providing uniform irradiation and short diffusion length. It can enhance the conversion efficiency of photocatalysis due to the simple spatial symmetry inside the microreactor channel and among the individual channels. In addition, the bandgap of TiO2, ZnO, or other photocatalyst nanoparticles with symmetric crystal structure can be modified through doping or embedding. In this mini-review, a review of the reported continuous-flow photocatalytic microfluidic reactor is discussed from the perspective of both microreactor design and material engineering.

Studies on the structure and function of the mammalian testis V. Steroid metabolism by isolated interstitium and seminiferous tubules of the human testis

1974 ◽  
Vol 186 (1083) ◽  
pp. 99-120 ◽  
Keyword(s):  
Metabolic Activity ◽  
General Pattern ◽  
Steroid Metabolism ◽  
Human Testis ◽  
Clinical Aspects ◽  
Seminiferous Tubules ◽  
Bicarbonate Buffer ◽  
The Media ◽  
The Individual ◽  
And Function

Tissue was obtained from the testes of three men, two in the age range 72-75 years (subjects A and B) and one aged 25 years (subject C). Parts of the testes were dissected to obtain samples of interstitium and tubules. The individual components and whole tissue were each incubated with equimolar concentrations of [7 α - 3 H]pregnenolone and [4- 14 C]progesterone in Krebs-Ringer bicarbonate buffer pH 7.4, at 35 °C with the addition of glucose but without cofactors. Some incubations were carried out with the substrates [4- 14 C]androstenedione and [7 α - 3 H]testosterone. The media were extracted both at various time intervals throughout the incubation for a kinetic study of the metabolic activity and after a fixed interval of time at the end of the incubations. In some incubations with whole tissue both media and tissue were extracted. Both the tubules and interstitium displayed steroid metabolic activity. Qualitatively they yielded the same range of metabolites, one series leading to the formation of testosterone (∆ 5 pathway) and the other to a variety of C 21 compounds as represented by 5 α -pregnan-3 β -ol-20-one. With similar amounts of tissue there was little difference in the yields of the main products formed by the tubules as compared with those formed by the interstitium; in incubations with [4- 14 C]androstenedione the rate of conversion to [ 14 C ]testosterone by the tubules greatly exceeded that due to the interstitium. Marked differences were found in the pattern of steroid metabolism by whole tissue as compared to the general pattern presented by the corresponding tubules and interstitium. It is concluded that the seminiferous tubules and interstitium of the human testis are both capable of steroid metabolism and hence that whole tissue incubations alone are of limited value and could give rise to misleading data. Some clinical aspects of the results are briefly discussed.

scholarly journals Postprenylation CAAX Processing Is Required for Proper Localization of Ras but Not Rho GTPases

2005 ◽  
Vol 16 (4) ◽  
pp. 1606-1616 ◽  
Author(s):  
David Michaelson ◽  
Wasif Ali ◽  
Vi K. Chiu ◽  
Martin Bergo ◽  
Joseph Silletti ◽  
...  
Keyword(s):  
Posttranslational Modifications ◽  
Protein Trafficking ◽  
Rho Gtpases ◽  
Ras Proteins ◽  
Rho Proteins ◽  
Carboxyl Methylation ◽  
C Terminus ◽  
Individual Contributions ◽  
The Individual ◽  
And Function

The CAAX motif at the C terminus of most monomeric GTPases is required for membrane targeting because it signals for a series of three posttranslational modifications that include isoprenylation, endoproteolytic release of the C-terminal– AAX amino acids, and carboxyl methylation of the newly exposed isoprenylcysteine. The individual contributions of these modifications to protein trafficking and function are unknown. To address this issue, we performed a series of experiments with mouse embryonic fibroblasts (MEFs) lacking Rce1 (responsible for removal of the –AAX sequence) or Icmt (responsible for carboxyl methylation of the isoprenylcysteine). In MEFs lacking Rce1 or Icmt, farnesylated Ras proteins were mislocalized. In contrast, the intracellular localizations of geranylgeranylated Rho GTPases were not perturbed. Consistent with the latter finding, RhoGDI binding and actin remodeling were normal in Rce1- and Icmt-deficient cells. Swapping geranylgeranylation for farnesylation on Ras proteins or vice versa on Rho proteins reversed the differential sensitivities to Rce1 and Icmt deficiency. These results suggest that postprenylation CAAX processing is required for proper localization of farnesylated Ras but not geranygeranylated Rho proteins.

The Tegument and Associated Structures of Fasciola Hepatica

1963 ◽  
Vol s3-104 (68) ◽  
pp. 505-512
Author(s):  
L. T. THREADGOLD
Keyword(s):  
Electron Microscopy ◽  
Surface Layer ◽  
Light Microscopy ◽  
Fasciola Hepatica ◽  
Golgi Bodies ◽  
New Knowledge ◽  
Pinocytotic Vesicles ◽  
The Individual ◽  
And Function ◽  
Internal Level

The cuticle of light microscopy is shown by electron microscopy to be a surface layer of protoplasm which is an extension of areas of nucleated protoplasm lying deep in the parenchyma. The cuticle therefore exists at two levels. The external level is syncytial, consisting of plateaux separated by branching valleys. This level contains apical pinocytotic vesicles, numerous mitochondria, endoplasmic membranes, large basal and other vacuoles, and dense spines. Tube-like evaginations from the base of the external level connect it to the individual areas of flask-shaped protoplasm which compose the internal level. Each of these areas of protoplasm contains a nucleus, great numbers of mitochondria, some vacuoles and diffuse inclusions, and the Golgi bodies. The histochemistry and function of the cuticle is discussed in the light of this new knowledge of cuticular ultrastructure, and a comparison is made between the cuticle of Cestoda and Trematoda.

Predicting Chromosome Flexibility from the Genomic Sequence Based on Deep Learning Neural Networks

2021 ◽  
Vol 16 ◽  
Author(s):  
Jinghao Peng ◽  
Jiajie Peng ◽  
Haiyin Piao ◽  
Zhang Luo ◽  
Kelin Xia ◽  
...  
Keyword(s):  
Deep Learning ◽  
High Performance ◽  
Genomic Sequence ◽  
Sequence Data ◽  
Function Analysis ◽  
Double Helix ◽  
Gm12878 Cell ◽  
Genomic Sequence Analysis ◽  
And Function ◽  
Nuclear Processes

Background: The open and accessible regions of the chromosome are more likely to be bound by transcription factors which are important for nuclear processes and biological functions. Studying the change of chromosome flexibility can help to discover and analyze disease markers and improve the efficiency of clinical diagnosis. Current methods for predicting chromosome flexibility based on Hi-C data include the flexibility-rigidity index (FRI) and the Gaussian network model (GNM), which have been proposed to characterize chromosome flexibility. However, these methods require the chromosome structure data based on 3D biological experiments, which is time-consuming and expensive. Objective: Generally, the folding and curling of the double helix sequence of DNA have a great impact on chromosome flexibility and function. Motivated by the success of genomic sequence analysis in biomolecular function analysis, we hope to propose a method to predict chromosome flexibility only based on genomic sequence data. Method: We propose a new method (named "DeepCFP") using deep learning models to predict chromosome flexibility based on only genomic sequence features. The model has been tested in the GM12878 cell line. Results: The maximum accuracy of our model has reached 91%. The performance of DeepCFP is close to FRI and GNM. Conclusion: The DeepCFP can achieve high performance only based on genomic sequence.

Effect of EPS on biofilm structure and function as revealed by an individual-based model of biofilm growth

2001 ◽  
Vol 43 (6) ◽  
pp. 135-135 ◽  
Author(s):  
J.-U. Kreft ◽  
J. W. Wimpenny
Keyword(s):  
Dimensional Space ◽  
Three Dimensional ◽  
Structure And Function ◽  
Individual Based Model ◽  
Biofilm Growth ◽  
Rate Dependent ◽  
Binding Forces ◽  
The Individual ◽  
And Function ◽  
Biofilm Structure

We have simulated a nitrifying biofilm with one ammonia and one nitrite oxidising species in order to elucidate the effect of various extracellular polymeric substance (EPS) production scenarios on biofilm structure and function. The individual-based model (IbM) BacSim simulates diffusion of all substrates on a two-dimensional lattice. Each bacterium is individually simulated as a sphere of given size in a continuous, three-dimensional space. EPS production kinetics was described by a growth rate dependent and an independent term (Luedeking-Piret equation). The structure of the biofilm was dramatically influenced by EPS production or capsule formation. EPS production decreased growth of producers and stimulated growth of non-producers because of the energy cost involved. For the same reason, EPS accumulation can fall as its rate of production increases. The patchiness and roughness of the biofilm decreased and the porosity increased due to EPS production. EPS density was maximal in the middle of the vertical profile. Introduction of binding forces between like cells increased clustering.

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