scholarly journals A unique invertase is important for sugar absorption of an obligate biotrophic pathogen during infection

2017 ◽  
Vol 215 (4) ◽  
pp. 1548-1561 ◽  
Author(s):  
Qing Chang ◽  
Jie Liu ◽  
Xiaohong Lin ◽  
Shoujun Hu ◽  
Yang Yang ◽  
...  
1996 ◽  
Vol 110 (5) ◽  
pp. 1669-1674
Author(s):  
HV Carey
Keyword(s):  

1991 ◽  
Vol 260 (3) ◽  
pp. G457-G463 ◽  
Author(s):  
M. Moreto ◽  
C. Amat ◽  
A. Puchal ◽  
R. K. Buddington ◽  
J. M. Planas

We examined the characteristics of amino acid and sugar absorption by the proximal cecum (PC) of chickens during posthatch development. Rates of absorption of L-proline (Pro) and alpha-methyl-D-glucoside (MG) were measured at 2 days, 5 wk, and 13 wk after hatch with an in vitro everted-sleeve method. For each age, pieces of PC and midjejunum were incubated in solutions containing 0.1-50 mM Pro or MG, and the active and passive components of Pro and MG absorption were determined. Five conclusions may be stated. 1) There are two carrier-mediated transport systems for Pro in the PC: a higher capacity Na(+)-dependent system (Vmax between 1.6 and 3.2 nmol.mg-1.min-1), and a lower capacity Na(+)-independent system (Vmax 0.3-0.8 nmol.mg-1.min-1). 2) Whereas both Pro transport systems are present in the PC at 5 and 13 wk, only the Na(+)-dependent system was found at 2 days. Although rates of transport per milligram tissue by the Na(+)-dependent system fell during development, when rates were normalized to nominal surface area, Vmax was significantly higher in the 5-wk-old group than in the other groups. 3) MG transport is by a Na(+)-dependent system. Vmax values (nmol.mg-1.min-1) were 0.32 (2 days), less than 0.43 (5 wk), and = 0.55 (13 wk). These differences were not affected by normalization to surface area. 4) Because at physiological concentrations passive influx of Pro and MG would be negligible, absorption of amino acids and sugars by the PC would be dependent on the presence of carrier-mediated systems.(ABSTRACT TRUNCATED AT 250 WORDS)


1969 ◽  
Vol 22 (3) ◽  
pp. 311-314 ◽  
Author(s):  
DAVID FROMM
Keyword(s):  

JAMA ◽  
1924 ◽  
Vol 82 (20) ◽  
pp. 1595 ◽  
Author(s):  
RANDOLPH G. FLOOD
Keyword(s):  

1958 ◽  
Vol 36 (3) ◽  
pp. 347-362 ◽  
Author(s):  
E. Riklis ◽  
J. H. Quastel

The rate of absorption of glucose from isolated surviving guinea pig intestine increases with increase of the concentration of glucose in the lumen until a maximum rate is obtained. The relation between absorption rate of glucose and initial glucose concentration conforms to an equation of the Michaelis–Menten type. The apparent Km(half saturation concentration) is 7 × 10−3M. Increase of the concentration of potassium ions in the Ringer–bicarbonate solution bathing the intestine leads to an increase of the rate of glucose absorption, this being most marked with 15.6 meq./liter K+and 14 mM glucose. No such stimulating action of potassium ions is observed on glucose absorption under anaerobic conditions. The effect of increased potassium ion concentration is to accelerate the rate of transport found with low concentrations of glucose to the maximum value found with high concentrations of the sugar. Sodium ions must be present for glucose absorption to take place and omission of magnesium ions from a Ringer–bicarbonate solution, containing 15.6 meq./liter K+, brings about a decreased rate of active glucose transport. Magnesium ions are necessary for the stimulated rate of glucose absorption obtained in the presence of potassium ions. The presence of ammonium ions decreases the rate of glucose absorption. Potassium ions may be effectively replaced by rubidium ions for stimulation of glucose transport. Cesium ions do not activate. The proportion of glucose to fructose appearing in the serosal solution, when fructose is absorbed from the mucosal solution, depends on the concentration of fructose present. The proportion may be as high as 9:1 with low (7 mM) fructose concentrations; it decreases with increasing fructose concentrations. The active transport of fructose, as demonstrated by the conversion of fructose in the isolated surviving guinea pig intestine, is enhanced by the presence of potassium ions (15.6 meq./liter). The rate of transport of fructose itself is unaffected by potassium. Using radioactive glucose and fructose, it is shown that the total amount of sugar transferred through the intestine as estimated by the radioactivity appearing in the serosal solution is approximately that calculated from chemical analyses. Potassium ions have no activating action on the transport of sugars such as sorbose, mannose, and D-glucosamine, but have a marked effect on galactose transport. The results support the conclusion that potassium ions do not influence active transport of glucose, fructose, and galactose by a change of intestinal permeability to these sugars, but do so by affecting a specific phase involved in the mechanism of active transport of sugars. The presence of L-glutamine stimulates active transport of glucose, whereas that of L-glutamate tends to diminish it.


1968 ◽  
Vol 61 (11P1) ◽  
pp. 1095-1099 ◽  
Author(s):  
A Holzel
Keyword(s):  

Author(s):  
F M van Overbeek ◽  
R M van Elburf ◽  
J J Uil ◽  
CJJ Mulder ◽  
HSA Heymans

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