scholarly journals Arabidopsis GOLDEN2-LIKE (GLK) transcription factors activate jasmonic acid (JA)-dependent disease susceptibility to the biotrophic pathogen Hyaloperonospora arabidopsidis, as well as JA-independent plant immunity against the necrotrophic pathogen Botryti

2013 ◽  
Vol 15 (2) ◽  
pp. 174-184 ◽  
Author(s):  
Jhadeswar Murmu ◽  
Michael Wilton ◽  
Ghislaine Allard ◽  
Radhey Pandeya ◽  
Darrell Desveaux ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Jasmonic Acid ◽  
Disease Susceptibility ◽  
Plant Immunity ◽  
Necrotrophic Pathogen ◽  
Biotrophic Pathogen ◽  
Hyaloperonospora Arabidopsidis

scholarly journals JA signal-mediated immunity of Dendrobium catenatum to necrotrophic Southern Blight pathogen

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Cong Li ◽  
Qiuyi Shen ◽  
Xiang Cai ◽  
Danni Lai ◽  
Lingshang Wu ◽  
...  
Keyword(s):  
Large Scale ◽  
Plant Immunity ◽  
Expression Patterns ◽  
Protein Domain ◽  
Functional Identification ◽  
Necrotrophic Pathogen ◽  
Southern Blight ◽  
Meja Treatment ◽  
Sclerotium Delphinii ◽  
Large Scale Cultivation

Abstract Background Dendrobium catenatum belongs to the Orchidaceae, and is a precious Chinese herbal medicine. In the past 20 years, D. catenatum industry has developed from an endangered medicinal plant to multi-billion dollar grade industry. The necrotrophic pathogen Sclerotium delphinii has a devastating effection on over 500 plant species, especially resulting in widespread infection and severe yield loss in the process of large-scale cultivation of D. catenatum. It has been widely reported that Jasmonate (JA) is involved in plant immunity to pathogens, but the mechanisms of JA-induced plant resistance to S. delphinii are unclear. Results In the present study, the role of JA in enhancing D. catenatum resistance to S. delphinii was investigated. We identified 2 COI1, 13 JAZ, and 12 MYC proteins in D. catenatum genome. Subsequently, systematic analyses containing phylogenetic relationship, gene structure, protein domain, and motif architecture of core JA pathway proteins were conducted in D. catenatum and the newly characterized homologs from its closely related orchid species Phalaenopsis equestris and Apostasia shenzhenica, along with the well-investigated homologs from Arabidopsis thaliana and Oryza sativa. Public RNA-seq data were investigated to analyze the expression patterns of D. catenatum core JA pathway genes in various tissues and organs. Transcriptome analysis of MeJA and S. delphinii treatment showed exogenous MeJA changed most of the expression of the above genes, and several key members, including DcJAZ1/2/5 and DcMYC2b, are involved in enhancing defense ability to S. delphinii in D. catenatum. Conclusions The findings indicate exogenous MeJA treatment affects the expression level of DcJAZ1/2/5 and DcMYC2b, thereby enhancing D. catenatum resistance to S. delphinii. This research would be helpful for future functional identification of core JA pathway genes involved in breeding for disease resistance in D. catenatum.

scholarly journals Multiple Candidate Effectors from the Oomycete Pathogen Hyaloperonospora arabidopsidis Suppress Host Plant Immunity

2011 ◽  
Vol 7 (11) ◽  
pp. e1002348 ◽  
Author(s):  
Georgina Fabro ◽  
Jens Steinbrenner ◽  
Mary Coates ◽  
Naveed Ishaque ◽  
Laura Baxter ◽  
...  
Keyword(s):  
Host Plant ◽  
Plant Immunity ◽  
Oomycete Pathogen ◽  
Hyaloperonospora Arabidopsidis

scholarly journals Abscisic acid is essential for rewiring of jasmonic acid-dependent defenses during herbivory

2019 ◽  
Author(s):  
Irene A Vos ◽  
Adriaan Verhage ◽  
Lewis G Watt ◽  
Ido Vlaardingerbroek ◽  
Robert C Schuurink ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Jasmonic Acid ◽  
Plant Hormone ◽  
Pieris Rapae ◽  
Necrotrophic Pathogen ◽  
Plant Insect Interactions ◽  
Gcc Box ◽  
Insect Interactions ◽  
And Control

AbstractJasmonic acid (JA) is an important plant hormone in the regulation of defenses against chewing herbivores and necrotrophic pathogens. In Arabidopsis thaliana, the JA response pathway consists of two antagonistic branches that are regulated by MYC- and ERF-type transcription factors, respectively. The role of abscisic acid (ABA) and ethylene (ET) in the molecular regulation of the MYC/ERF antagonism during plant-insect interactions is still unclear. Here, we show that production of ABA induced in response to leaf-chewing Pieris rapae caterpillars is required for both the activation of the MYC-branch and the suppression of the ERF-branch during herbivory. Exogenous application of ABA suppressed ectopic ERF-mediated PDF1.2 expression in 35S::ORA59 plants. Moreover, the GCC-box promoter motif, which is required for JA/ET-induced activation of the ERF-branch genes ORA59 and PDF1.2, was targeted by ABA. Application of gaseous ET counteracted activation of the MYC-branch and repression of the ERF-branch by P. rapae, but infection with the ET-inducing necrotrophic pathogen Botrytis cinerea did not. Accordingly, P. rapae performed equally well on B. cinerea-infected and control plants, whereas activation of the MYC-branch resulted in reduced caterpillar performance. Together, these data indicate that upon feeding by P. rapae, ABA is essential for activating the MYC-branch and suppressing the ERF-branch of the JA pathway, which maximizes defense against caterpillars.

scholarly journals Calcium/Calmodulin-Mediated Defense Signaling: What Is Looming on the Horizon for AtSR1/CAMTA3-Mediated Signaling in Plant Immunity

2022 ◽  
Vol 12 ◽  
Author(s):  
Peiguo Yuan ◽  
Kiwamu Tanaka ◽  
B. W. Poovaiah
Keyword(s):  
Transcription Factors ◽  
Plant Immunity ◽  
Control Plant ◽  
Plant Cells ◽  
Regulatory Mechanisms ◽  
Early Event ◽  
Gradual Change ◽  
Microbe Interactions ◽  
Immune Related Genes ◽  
Stressful Environments

Calcium (Ca2+) signaling in plant cells is an essential and early event during plant-microbe interactions. The recognition of microbe-derived molecules activates Ca2+ channels or Ca2+ pumps that trigger a transient increase in Ca2+ in the cytoplasm. The Ca2+ binding proteins (such as CBL, CPK, CaM, and CML), known as Ca2+ sensors, relay the Ca2+ signal into down-stream signaling events, e.g., activating transcription factors in the nucleus. For example, CaM and CML decode the Ca2+ signals to the CaM/CML-binding protein, especially CaM-binding transcription factors (AtSRs/CAMTAs), to induce the expressions of immune-related genes. In this review, we discuss the recent breakthroughs in down-stream Ca2+ signaling as a dynamic process, subjected to continuous variation and gradual change. AtSR1/CAMTA3 is a CaM-mediated transcription factor that represses plant immunity in non-stressful environments. Stress-triggered Ca2+ spikes impact the Ca2+-CaM-AtSR1 complex to control plant immune response. We also discuss other regulatory mechanisms in which Ca2+ signaling activates CPKs and MAPKs cascades followed by regulating the function of AtSR1 by changing its stability, phosphorylation status, and subcellular localization during plant defense.

scholarly journals Chitosan Oligosaccharide Induces Resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana by Activating Both Salicylic Acid– and Jasmonic Acid–Mediated Pathways

2018 ◽  
Vol 31 (12) ◽  
pp. 1271-1279 ◽  
Author(s):  
Xiaochen Jia ◽  
Haihong Zeng ◽  
Wenxia Wang ◽  
Fuyun Zhang ◽  
Heng Yin
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Induced Resistance ◽  
Pseudomonas Syringae ◽  
Plant Immunity ◽  
Chitosan Oligosaccharide ◽  
Induction Effect ◽  
Tomato Dc3000 ◽  
Expression Of Genes ◽  
Underlying Mechanisms

Chitosan oligosaccharide (COS) is an effective plant immunity elicitor; however, its induction mechanism in plants is complex and needs further investigation. In this study, the Arabidopsis–Pseudomonas syringae pv. tomato DC3000 (hereafter called DC3000) interaction was used to investigate the induction effect and the underlying mechanisms of COS. COS is effective in inducing resistance to DC3000 in Arabidopsis, and our results demonstrate that treatment with COS 3 days before DC3000 inoculation provided the most effective resistance. Disease severity in jar1 (jasmonic acid [JA]-deficient mutant), NahG, and sid2 (salicylic acid [SA]-deficient mutants) suggest both the SA and JA pathways are required for the Arabidopsis response to DC3000. COS pretreatment induced resistance in wild type (WT), jar1, and also, although to a lesser degree, in NahG and sid2 plants, implying that the SA and JA pathways play redundant roles in COS-induced resistance to DC3000. In COS-pretreated plants, expression of genes related to the SA pathway (PR1, PR2, and PR5) and SA content increased in both WT and jar1. Moreover, expression of genes related to the JA pathway (PDF1.2 and VSP2) and JA content both increased in WT and NahG. In conclusion, COS induces resistance to DC3000 in Arabidopsis by activating both SA- and JA-mediated pathways, although SA and JA pathways play redundant roles in this COS-induced resistance.

scholarly journals Functions of Jasmonic Acid in Plant Regulation and Response to Abiotic Stress

2020 ◽  
Vol 21 (4) ◽  
pp. 1446 ◽  
Author(s):  
Jia Wang ◽  
Li Song ◽  
Xue Gong ◽  
Jinfan Xu ◽  
Minhui Li
Keyword(s):  
Transcription Factors ◽  
Abiotic Stress ◽  
Jasmonic Acid ◽  
Signaling Pathways ◽  
Signaling Pathway ◽  
Large Scale ◽  
Higher Plants ◽  
Complex Signal ◽  
Ja Signaling ◽  
Signal Network

Jasmonic acid (JA) is an endogenous growth-regulating substance, initially identified as a stress-related hormone in higher plants. Similarly, the exogenous application of JA also has a regulatory effect on plants. Abiotic stress often causes large-scale plant damage. In this review, we focus on the JA signaling pathways in response to abiotic stresses, including cold, drought, salinity, heavy metals, and light. On the other hand, JA does not play an independent regulatory role, but works in a complex signal network with other phytohormone signaling pathways. In this review, we will discuss transcription factors and genes involved in the regulation of the JA signaling pathway in response to abiotic stress. In this process, the JAZ-MYC module plays a central role in the JA signaling pathway through integration of regulatory transcription factors and related genes. Simultaneously, JA has synergistic and antagonistic effects with abscisic acid (ABA), ethylene (ET), salicylic acid (SA), and other plant hormones in the process of resisting environmental stress.

scholarly journals Molecular Cloning and Functional Characterization of CpMYC2 and CpBHLH13 Transcription Factors from Wintersweet (Chimonanthus praecox L.)

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 785
Author(s):  
Muhammad Zeshan Aslam ◽  
Xiang Lin ◽  
Xiang Li ◽  
Nan Yang ◽  
Longqing Chen
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Transgenic Plants ◽  
Jasmonic Acid ◽  
Volatile Compounds ◽  
Floral Scent ◽  
Functional Characterization ◽  
High Expression ◽  
Floral Volatile ◽  
Chimonanthus Praecox

Wintersweet (Chimonanthus praecox L.) is an ornamental and economically significant shrub known for its unique flowering characteristics, especially the emission of abundant floral volatile organic compounds. Thus, an understanding of the molecular mechanism of the production of these compounds is necessary to create new breeds with high volatile production. In this study, two bHLH transcription factors (CpMYC2 and CpbHLH13) of Wintersweet H29 were functionally characterized to illustrate their possible role in the production of volatile compounds. The qRT-PCR results showed that the expression of CpMYC2 and CpbHLH13 increased from the flower budding to full bloom stage, indicating that these two genes may play an essential role in blooming and aroma production in wintersweet. Gas chromatography-mass spectroscopy (GC-MS) analysis revealed that the overexpression of CpMYC2 in arabidopsis (Arabidopsis thaliana) AtMYC2-2 mutant (Salk_083483) and tobacco (Nicotiana tabaccum) genotype Petit Havana SR1 significantly increased floral volatile monoterpene, especially linalool, while the overexpression of CpbHLH13 in Arabidopsis thaliana ecotype Columbia-0 (Col-0) and tobacco genotype SR1 increased floral sesquiterpene β-caryophyllene production in both types of transgenic plants respectively. High expression of terpene synthase (TPS) genes in transgenic A. thaliana along with high expression of CpMYC2 and CpbHLH13 in transgenic plants was also observed. The application of a combination of methyl jasmonic acid (MeJA) and gibberellic acid (GA3) showed an increment in linalool production in CpMYC2-overexpressing arabidopsis plants, and the high transcript level of TPS genes also suggested the involvement of CpMYC2 in the jasmonic acid (JA) signaling pathway. These results indicate that both the CpMYC2 and CpbHLH13 transcription factors of wintersweet are possibly involved in the positive regulation and biosynthesis of monoterpene (linalool) and sesquiterpene (β-caryophyllene) in transgenic plants. This study also indicates the potential application of wintersweet as a valuable genomic material for the genetic modification of floral scent in other flowering plants that produce less volatile compounds.

Role of P-type ATPase metal transporters and plant immunity induced by jasmonic acid against Lead (Pb) toxicity in tomato

2019 ◽  
Vol 174 ◽  
pp. 283-294 ◽  
Author(s):  
Shagun Bali ◽  
Vijay Lakshmi Jamwal ◽  
Parminder Kaur ◽  
Sukhmeen Kaur Kohli ◽  
Puja Ohri ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Plant Immunity ◽  
Metal Transporters ◽  
Pb Toxicity ◽  
P Type
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