Changes in intestinal barrier protein expression and intestinal flora in a rat model of visceral hypersensitivity

2021 ◽  
Author(s):  
Li Zhao ◽  
Peipei Ren ◽  
Miaolei Wang ◽  
Jingjing Wang ◽  
Xueyun He ◽  
...  
Keyword(s):  
Protein Expression ◽  
Rat Model ◽  
Intestinal Flora ◽  
Intestinal Barrier ◽  
Visceral Hypersensitivity

scholarly journals Sini-San Regulates the NO-cGMP-PKG Pathway in the Spinal Dorsal Horn in a Modified Rat Model of Functional Dyspepsia

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Zhenyu Wu ◽  
Xiaofang Lu ◽  
Shengsheng Zhang ◽  
Chunyang Zhu
Keyword(s):  
Gene Expression ◽  
Dorsal Horn ◽  
Functional Dyspepsia ◽  
Rat Model ◽  
Spinal Dorsal Horn ◽  
Visceral Hypersensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gastric Distention ◽  
Real Time Quantitative Pcr ◽  
Spinal Dorsal

The present study investigated the effect of Chinese medicine Sini-San (SNS) on visceral hypersensitivity in a rat model of functional dyspepsia (FD), and it explored related underlying mechanisms. The rat model of FD was developed by combining neonatal iodoacetamide (IA) treatment and adult tail-clamping. After SNS treatment, the behavior and electromyographic testing were performed to evaluate the visceromotor responses of rats to gastric distention. Immunofluorescence was used to detect the distribution of iNOS-positive cells in the spinal dorsal horn, while the real-time quantitative PCR and western blot were used for detection of the gene expression of c-fos, iNOS, and GABAb and protein levels of iNOS and GABAb in the spinal dorsal horn, respectively. The protein concentration of cGMP and PKG proteins in the spinal dorsal horn were quantified by enzyme-linked immunosorbent assay. In this study, SNS treatment significantly reduced the behavioral score and electromyographic response to graded intragastric distension pressure. The middle-dose of SNS treatment significantly reduced the distribution of iNOS-positive cells in the spinal dorsal horn of FD model rats. The gene expression of c-fos, iNOS, and GABAb and the protein contents of iNOS, GABAb, cGMP, and PKG in the spinal dorsal horn of FD model rats were restored to a normal level by middle-dose of SNS treatment. Our results suggest that Sini-San may alleviate the visceral hypersensitivity in FD model rats via regulation of the NO/cGMP/PKG pathway in the spinal dorsal horn.

Changes in adiponectin expression in acute myocardial infarction rats and the significance of bisoprolol intervention

2011 ◽  
Vol 89 (2) ◽  
pp. 109-115 ◽  
Author(s):  
Song Zhang ◽  
Ben He ◽  
Steven Goldstein ◽  
Junbo Ge ◽  
Zuyue Wang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Protein Expression ◽  
Reverse Transcriptase ◽  
Rat Model ◽  
Protective Factor ◽  
Sham Operation ◽  
Rt Pcr ◽  
Sham Operation Group ◽  
Operation Group

The aims of this study were to explore the changes in expression of myocardial adiponectin (APN), changes in serum APN, and the significance of bisoprolol intervention in acute myocardial infarction (AMI) rats. An AMI rat model was established for the purposes of this study and was used for analysis of serum APN as determined by ELISA. Changes in expression of myocardial APN mRNA and APN protein in AMI rats were determined via reverse transcriptase (RT)–PCR and immunohistochemistry. Serum APN concentration and APN protein expression of the myocardium decreased significantly in the AMI groups compared with the sham operation group, with the lowest serum APN and APN protein expression on day 7 after AMI. On days 7 and 10 after AMI, the expression of myocardial APN mRNA in the AMI groups decreased significantly compared with the sham operation group. However, the APN mRNA increased on day 10 compared with that on day 7. Notably, there was an increase in levels of serum APN and myocardial APN expression after bisoprolol intervention. The expression of myocardial APN and serum APN decreased in AMI rats. APN may be an important protective factor against AMI. Bisoprolol can also protect against AMI because it increases APN expression.

scholarly journals Schisandra chinensis reverses visceral hypersensitivity in a neonatal–maternal separated rat model

Phytomedicine ◽  
2012 ◽  
Vol 19 (5) ◽  
pp. 402-408 ◽  
Author(s):  
Jia-Ming Yang ◽  
Yan-Fang Xian ◽  
Paul S.P. Ip ◽  
Justin C.Y. Wu ◽  
Lixing Lao ◽  
...  
Keyword(s):  
Rat Model ◽  
Visceral Hypersensitivity ◽  
Schisandra Chinensis

scholarly journals 7 The Peripheral Histamine 1-Receptor Antagonist Fexofenadine Effectively Reverses Stress-Induced Visceral Hypersensitivity in a Rat Model of Maternal Separation

2010 ◽  
Vol 138 (5) ◽  
pp. S-1 ◽  
Author(s):  
Oana I. Stanisor ◽  
Sophie A. van Diest ◽  
Olaf Welting ◽  
Cathy Cailotto ◽  
Jan van der Vliet ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Rat Model ◽  
Maternal Separation ◽  
Visceral Hypersensitivity

Tu2116 The Effects of Jnj 7777120, a Selective Histamine H4 Receptor Antagonist, on Visceromotor Responses in a Rat Model for Post-Inflammatory Visceral Hypersensitivity

2013 ◽  
Vol 144 (5) ◽  
pp. S-934 ◽  
Author(s):  
Annemie Deiteren ◽  
Joris G. De Man ◽  
Nathalie E. Ruyssers ◽  
Tom G. Moreels ◽  
Paul A. Pelckmans ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Rat Model ◽  
Visceral Hypersensitivity ◽  
Histamine H4 Receptor ◽  
H4 Receptor
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