scholarly journals Neonatal Maternal Deprivation Enhances Presynaptic P2X7 Receptor Transmission in Insular Cortex in an Adult Rat Model of Visceral Hypersensitivity

2016 ◽  
Vol 23 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Ping-An Zhang ◽  
Qi-Ya Xu ◽  
Lu Xue ◽  
Hang Zheng ◽  
Jun Yan ◽  
...  
Keyword(s):  
Rat Model ◽  
P2x7 Receptor ◽  
Insular Cortex ◽  
Maternal Deprivation ◽  
Visceral Hypersensitivity ◽  
Adult Rat

scholarly journals Sini-San Regulates the NO-cGMP-PKG Pathway in the Spinal Dorsal Horn in a Modified Rat Model of Functional Dyspepsia

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Zhenyu Wu ◽  
Xiaofang Lu ◽  
Shengsheng Zhang ◽  
Chunyang Zhu
Keyword(s):  
Gene Expression ◽  
Dorsal Horn ◽  
Functional Dyspepsia ◽  
Rat Model ◽  
Spinal Dorsal Horn ◽  
Visceral Hypersensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gastric Distention ◽  
Real Time Quantitative Pcr ◽  
Spinal Dorsal

The present study investigated the effect of Chinese medicine Sini-San (SNS) on visceral hypersensitivity in a rat model of functional dyspepsia (FD), and it explored related underlying mechanisms. The rat model of FD was developed by combining neonatal iodoacetamide (IA) treatment and adult tail-clamping. After SNS treatment, the behavior and electromyographic testing were performed to evaluate the visceromotor responses of rats to gastric distention. Immunofluorescence was used to detect the distribution of iNOS-positive cells in the spinal dorsal horn, while the real-time quantitative PCR and western blot were used for detection of the gene expression of c-fos, iNOS, and GABAb and protein levels of iNOS and GABAb in the spinal dorsal horn, respectively. The protein concentration of cGMP and PKG proteins in the spinal dorsal horn were quantified by enzyme-linked immunosorbent assay. In this study, SNS treatment significantly reduced the behavioral score and electromyographic response to graded intragastric distension pressure. The middle-dose of SNS treatment significantly reduced the distribution of iNOS-positive cells in the spinal dorsal horn of FD model rats. The gene expression of c-fos, iNOS, and GABAb and the protein contents of iNOS, GABAb, cGMP, and PKG in the spinal dorsal horn of FD model rats were restored to a normal level by middle-dose of SNS treatment. Our results suggest that Sini-San may alleviate the visceral hypersensitivity in FD model rats via regulation of the NO/cGMP/PKG pathway in the spinal dorsal horn.

scholarly journals Schisandra chinensis reverses visceral hypersensitivity in a neonatal–maternal separated rat model

Phytomedicine ◽  
2012 ◽  
Vol 19 (5) ◽  
pp. 402-408 ◽  
Author(s):  
Jia-Ming Yang ◽  
Yan-Fang Xian ◽  
Paul S.P. Ip ◽  
Justin C.Y. Wu ◽  
Lixing Lao ◽  
...  
Keyword(s):  
Rat Model ◽  
Visceral Hypersensitivity ◽  
Schisandra Chinensis

scholarly journals 7 The Peripheral Histamine 1-Receptor Antagonist Fexofenadine Effectively Reverses Stress-Induced Visceral Hypersensitivity in a Rat Model of Maternal Separation

2010 ◽  
Vol 138 (5) ◽  
pp. S-1 ◽  
Author(s):  
Oana I. Stanisor ◽  
Sophie A. van Diest ◽  
Olaf Welting ◽  
Cathy Cailotto ◽  
Jan van der Vliet ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Rat Model ◽  
Maternal Separation ◽  
Visceral Hypersensitivity

Tu2116 The Effects of Jnj 7777120, a Selective Histamine H4 Receptor Antagonist, on Visceromotor Responses in a Rat Model for Post-Inflammatory Visceral Hypersensitivity

2013 ◽  
Vol 144 (5) ◽  
pp. S-934 ◽  
Author(s):  
Annemie Deiteren ◽  
Joris G. De Man ◽  
Nathalie E. Ruyssers ◽  
Tom G. Moreels ◽  
Paul A. Pelckmans ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Rat Model ◽  
Visceral Hypersensitivity ◽  
Histamine H4 Receptor ◽  
H4 Receptor

scholarly journals P2X3 Receptor in Primary Afferent Neurons Mediates the Relief of Visceral Hypersensitivity by Electroacupuncture in an Irritable Bowel Syndrome Rat Model

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Fang Zhang ◽  
Zhe Ma ◽  
Zhijun Weng ◽  
Min Zhao ◽  
Handan Zheng ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Rat Model ◽  
Visceral Pain ◽  
Visceral Hypersensitivity ◽  
Receptor Protein ◽  
Resting Membrane Potential ◽  
Drg Neurons ◽  
Expression Levels ◽  
Behavioral Studies ◽  
Irritable Bowel

Background. Electroacupuncture (EA) has been confirmed effectiveness in the treatment of irritable bowel syndrome (IBS), and P2X3 receptors in the peripheral and central neurons participate in the acupuncture-mediated relief of the visceral pain in IBS. Objective. To reveal the neurobiological mechanism that P2X3 receptor of colonic primary sensory neurons in the dorsal root ganglia of the lumbosacral segment is involved in the alleviation of visceral hypersensitivity by EA in an IBS rat model. Methods. The IBS chronic visceral pain rat model was established according to the method of Al-Chaer et al. EA at the bilateral He-Mu points, including ST25 and ST37, was conducted for intervention. The behavioral studies, histopathology of colon, electrophysiology, immunofluorescence histochemistry, and real-time polymerase chain reaction assays were used to observe the role of P2X3 receptor in the colon and related DRG in relieving visceral hypersensitivity by EA. Results. EA significantly reduced the behavior scores of the IBS rats under different levels (20, 40, 60, 80 mmHg) of colorectal distention stimulation and downregulated the expression levels of P2X3 receptor protein and mRNA in colon and related DRG of the IBS rats. EA also regulated the electrical properties of the membranes, including the resting membrane potential, rheobase, and action potential of colon-associated DRG neurons in the IBS rats. Conclusion. EA can regulate the P2X3 receptor protein and mRNA expression levels in the colon and related DRG of IBS rats with visceral pain and then regulate the excitatory properties of DRG neurons.

Kainate receptor-mediated synaptic transmissions in the adult rodent insular cortex

2012 ◽  
Vol 108 (7) ◽  
pp. 1988-1998 ◽  
Author(s):  
Kohei Koga ◽  
Su-Eon Sim ◽  
Tao Chen ◽  
Long-Jun Wu ◽  
Bong-Kiun Kaang ◽  
...  
Keyword(s):  
Synaptic Transmission ◽  
Receptor Antagonist ◽  
Ampa Receptor ◽  
Time Course ◽  
Insular Cortex ◽  
Nmda Receptor Antagonist ◽  
Functional Roles ◽  
Adult Rat ◽  
Whole Cell Patch Clamp ◽  
The Central Nervous System

Kainate (KA) receptors are expressed widely in the central nervous system and regulate both excitatory and inhibitory synaptic transmission. KA receptors play important roles in fear memory, anxiety, and pain. However, little is known about their function in synaptic transmission in the insular cortex (IC), a critical region for taste, memory, and pain. Using whole cell patch-clamp recordings, we have shown that KA receptors contribute to fast synaptic transmission in neurons in all layers of the IC. In the presence of the GABAA receptor antagonist picrotoxin, the NMDA receptor antagonist AP-5, and the selective AMPA receptor antagonist GYKI 53655, KA receptor-mediated excitatory postsynaptic currents (KA EPSCs) were revealed. We found that KA EPSCs are ∼5–10% of AMPA/KA EPSCs in all layers of the adult mouse IC. Similar results were found in adult rat IC. KA EPSCs had a significantly slower rise time course and decay time constant compared with AMPA receptor-mediated EPSCs. High-frequency repetitive stimulations at 200 Hz significantly facilitated the summation of KA EPSCs. In addition, genetic deletion of GluK1 or GluK2 subunit partially reduced postsynaptic KA EPSCs, and exposure of GluK2 knockout mice to the selective GluK1 antagonist UBP 302 could significantly reduce the KA EPSCs. These data suggest that both GluK1 and GluK2 play functional roles in the IC. Our study may provide the synaptic basis for the physiology and pathology of KA receptors in the IC-related functions.

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