scholarly journals Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains

2021 ◽  
Author(s):  
Keiji Wakamatsu ◽  
Yoichi Chiba ◽  
Ryuta Murakami ◽  
Koichi Matsumoto ◽  
Yumi Miyai ◽  
...  
Keyword(s):  
Choroid Plexus ◽  
Immunohistochemical Expression ◽  
Human Brains

Immunoreactivities for hepcidin, ferroportin, and hephaestin in astrocytes and choroid plexus epithelium of human brains

2019 ◽  
Vol 40 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Ken Yanase ◽  
Naoya Uemura ◽  
Yoichi Chiba ◽  
Ryuta Murakami ◽  
Ryuji Fujihara ◽  
...  
Keyword(s):  
Choroid Plexus ◽  
Choroid Plexus Epithelium ◽  
Human Brains

scholarly journals Morphology of Human Nucleus Accumbens Neurons Based on the Immunohistochemical Expression of Gad67

2016 ◽  
Vol 17 (4) ◽  
pp. 297-302
Author(s):  
Maja Sazdanovic ◽  
Slobodanka Mitrovic ◽  
Milos Todorovic ◽  
Maja Vulovic ◽  
Dejan Jeremic ◽  
...  
Keyword(s):  
Nucleus Accumbens ◽  
Pyramidal Neurons ◽  
Immunohistochemical Analysis ◽  
Medium Spiny Neurons ◽  
Immunohistochemical Expression ◽  
Spiny Neurons ◽  
Different Types ◽  
Human Brains

Abstract The nucleus accumbens is a part of the ventral striatum along with the caudate nucleus and putamen. The role of the human nucleus accumbens in drug addiction and other psychiatric disorders is of great importance. The aim of this study was to characterize medium spiny neurons in the nucleus accumbens according to the immunohistochemical expression of GAD67. This study was conducted on twenty human brains of both sexes between the ages of 20 and 75. The expression of GAD67 was assessed immunohistochemically, and the characterization of the neurons was based on the shape and size of the soma and the number of impregnated primary dendrites. We showed that neurons of the human nucleus accumbens expressed GAD67 in the neuron soma and in the primary dendrites. An analysis of the cell body morphology revealed the following four different types of neurons: fusiform neurons, fusiform neurons with lateral dendrites, pyramidal neurons and multipolar neurons. An immunohistochemical analysis showed a strong GAD67 expression in GABAergic medium spiny neurons, which could be classifi ed into four different types, and these neurons morphologically correlated with those described by the Golgi study.

Immunoreactivity of urate transporters, GLUT9 and URAT1, is located in epithelial cells of the choroid plexus of human brains

2017 ◽  
Vol 659 ◽  
pp. 99-103 ◽  
Author(s):  
Naoya Uemura ◽  
Ryuta Murakami ◽  
Yoichi Chiba ◽  
Ken Yanase ◽  
Ryuji Fujihara ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Choroid Plexus ◽  
Human Brains

Organization of tight junctions in the choroid plexus epithelium

1978 ◽  
Vol 36 (2) ◽  
pp. 336-337
Author(s):  
B. Van Deurs ◽  
J. K. Koehler
Keyword(s):  
Choroid Plexus ◽  
Present Report ◽  
Freeze Fracture ◽  
Barrier Properties ◽  
Cell Junctions ◽  
Structural Basis ◽  
Apical Surface ◽  
Choroid Plexus Epithelium ◽  
Solid Nitrogen ◽  
Special Composition

The choroid plexus epithelium constitutes a blood-cerebrospinal fluid (CSF) barrier, and is involved in regulation of the special composition of the CSF. The epithelium is provided with an ouabain-sensitive Na/K-pump located at the apical surface, actively pumping ions into the CSF. The choroid plexus epithelium has been described as “leaky” with a low transepithelial resistance, and a passive transepithelial flux following a paracellular route (intercellular spaces and cell junctions) also takes place. The present report describes the structural basis for these “barrier” properties of the choroid plexus epithelium as revealed by freeze fracture.Choroid plexus from the lateral, third and fourth ventricles of rats were used. The tissue was fixed in glutaraldehyde and stored in 30% glycerol. Freezing was performed either in liquid nitrogen-cooled Freon 22, or directly in a mixture of liquid and solid nitrogen prepared in a special vacuum chamber. The latter method was always used, and considered necessary, when preparations of complementary (double) replicas were made.

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