Molecular detection of Wolbachia pipientis in natural populations of mosquito vectors of Dirofilaria immitis from continental Portugal: first detection in Culex theileri

2016 ◽  
Vol 30 (3) ◽  
pp. 301-309 ◽  
Author(s):  
V. DE PINHO MIXÃO ◽  
A. M. MENDES ◽  
I. L. MAURÍCIO ◽  
M. M. CALADO ◽  
M. T. NOVO ◽  
...  
Keyword(s):  
Dirofilaria Immitis ◽  
Molecular Detection ◽  
Natural Populations ◽  
Mosquito Vectors ◽  
Wolbachia Pipientis

Aedes vexans and Other Potential Mosquito Vectors of Dirofilaria immitis in Minnesota

1966 ◽  
Vol 52 (4) ◽  
pp. 762 ◽  
Author(s):  
William J. Bemrick ◽  
Hollace A. Sandholm
Keyword(s):  
Dirofilaria Immitis ◽  
Mosquito Vectors ◽  
Aedes Vexans

scholarly journals First Molecular Detection of Dirofilaria immitis and D. repens in Dogs from Kyrgyzstan

2020 ◽  
Vol 65 (4) ◽  
pp. 949-953
Author(s):  
Mehmet Fatih Aydın ◽  
Kürşat Altay ◽  
Ayperi Aytmirzakizi ◽  
Nazir Dumanlı
Keyword(s):  
Dirofilaria Immitis ◽  
Molecular Detection

scholarly journals Molecular Detection and Phylogenetic Analysis of Endosymbiont Wolbachia pipientis (Rickettsiales: Anaplasmataceae) Isolated from Dirofilaria immitis in Northwest of Iran

2019 ◽  
Author(s):  
Majid Khanmohammadi ◽  
Reza Falak ◽  
Ahmad Reza Meamar ◽  
Mehdi Arshadi ◽  
Lame Akhlaghi ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Sequences ◽  
Dirofilaria Immitis ◽  
Molecular Detection ◽  
Surface Protein ◽  
Coi Gene ◽  
Molecular Pattern ◽  
Filarial Nematodes ◽  
High Consistency ◽  
Wsp Gene

Background: The purpose of this study was molecular detection and phylogenetic analysis of Wolbachia species of Dirofilaria immitis. Methods: Adult filarial nematodes were collected from the cardiovascular and pulmonary arterial systems of natural­ly infected dogs, which caught in different geographical areas of Meshkin Shahr in Ardabil Province, Iran, during 2017. Dirofilaria immitis genomic DNA were extracted.  Phylogenetic analysis for proofing of D. immitis was car­ried out using cytochrome oxidase I (COI) gene. Afterward, the purified DNA was used to determine the molecular pattern of the Wolbachia surface protein (WSP) gene sequence by PCR. Results: Phylogeny and homology studies showed high consistency of the COI gene with the previously-registered sequences for D. immitis. Comparison of DNA sequences revealed no nucleotide variation between them. PCR showed that all of the collected parasites were infected with W. pipientis. The sequence of the WSP gene in Wolbach­ia species from D. immitis was significantly different from other species of Dirofilaria as well as other filarial spe­cies. The maximum homology was observed with the Wolbachia isolated from D. immitis. The greatest distance be­tween WSP nucleotides of Wolbachia species found between D. immitis and those isolated from Onchocerca lupi. Conclusion: PCR could be a simple but suitable method for detection of Wolbachia species. There is a pattern of host specificity between Wolbachia and Dirofilaria that can be related to ancestral evolutions. The results of this phylogenetic analysis and molecular characterization may help us for better identification of Wolbachia species and understanding of their coevolution.  

scholarly journals The Virulent Wolbachia Strain wMelPop Efficiently Establishes Somatic Infections in the Malaria Vector Anopheles gambiae

2009 ◽  
Vol 75 (10) ◽  
pp. 3373-3376 ◽  
Author(s):  
Chaoyang Jin ◽  
Xiaoxia Ren ◽  
Jason L. Rasgon
Keyword(s):  
Anopheles Gambiae ◽  
Malaria Vector ◽  
Wolbachia Strain ◽  
Mosquito Vectors ◽  
Wolbachia Pipientis

ABSTRACT Wolbachia pipientis bacteria are maternally inherited endosymbionts that are of interest to control the Anopheles mosquito vectors of malaria. Wolbachia does not infect Anopheles mosquitoes in nature, although cultured Anopheles cells can be infected. Here, we show that the virulent Wolbachia strain wMelPop can survive and replicate when injected into female Anopheles gambiae adults, but the somatic infections established are avirulent. These in vivo data suggest that stable Wolbachia infections of Anopheles may be possible.

Some potential mosquito vectors of the canine heartworm, Dirofilaria immitis, in the Calgary region of southern Alberta

1983 ◽  
Vol 61 (5) ◽  
pp. 1156-1158 ◽  
Author(s):  
Jack P. Frimeth ◽  
Hisao P. Arai
Keyword(s):  
Dirofilaria Immitis ◽  
Thoracic Cavity ◽  
Mosquito Vectors ◽  
Vector Species ◽  
Natural Conditions ◽  
Laboratory Rats ◽  
Complete Development ◽  
Prophylactic Measures ◽  
Southern Alberta ◽  
Third Stage

A total of 657 mosquitoes, representing eight species from the Calgary region of southern Alberta was fed on dogs infected with Dirofilaria immitis. Complete development to the infective stage occurred in Aedes campestris, A. cataphylla, A. flavescens, and A. vexans. The rate of development appeared dependent on the temperature, as well as on the vector species. This report is believed to be the first account on the development of D. immitis in the first three species. Third stage larvae recovered from A. flavescens and A. vexans, inoculated subcutaneously into either two dogs or two white laboratory rats, produced microfilaremias in both dogs and a single adult, male D. immitis in the thoracic cavity of one of the rats. It is suggested that these species may act as vectors under natural conditions and that the identification of the potential vectors of D. immitis in areas where it is currently not endemic is important since this knowledge can be used in the planning of prophylactic measures.

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