scholarly journals Characterization of 6S RNA in the Lyme disease spirochete

2019 ◽  
Vol 113 (2) ◽  
pp. 399-417 ◽  
Author(s):  
Dan Drecktrah ◽  
Laura S. Hall ◽  
Amanda J. Brinkworth ◽  
Jeanette R. Comstock ◽  
Karen M. Wassarman ◽  
...  
Keyword(s):  
Lyme Disease ◽  
6S Rna

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

scholarly journals Characterization of outer membranes isolated from Borrelia burgdorferi, the Lyme disease spirochete.

1995 ◽  
Vol 63 (6) ◽  
pp. 2154-2163 ◽  
Author(s):  
J D Radolf ◽  
M S Goldberg ◽  
K Bourell ◽  
S I Baker ◽  
J D Jones ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Outer Membranes

scholarly journals Characterization of 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidases from Borrelia burgdorferi: Antibiotic targets for Lyme disease

2020 ◽  
Vol 1864 (1) ◽  
pp. 129455 ◽  
Author(s):  
Kenneth A. Cornell ◽  
Reece J. Knippel ◽  
Gerald R. Cortright ◽  
Meghan Fonken ◽  
Christian Guerrero ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi

scholarly journals Identification and Functional Characterization of Complement Regulator-Acquiring Surface Protein 1 of the Lyme Disease Spirochetes Borrelia afzelii and Borrelia garinii

2005 ◽  
Vol 73 (4) ◽  
pp. 2351-2359 ◽  
Author(s):  
Reinhard Wallich ◽  
Joseph Pattathu ◽  
Veronique Kitiratschky ◽  
Christiane Brenner ◽  
Peter F. Zipfel ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Binding Site ◽  
Binding Protein ◽  
Functional Characterization ◽  
Surface Protein ◽  
Factor H ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Complement Regulator

ABSTRACT Complement regulator-acquiring surface protein 1 (CRASP-1) is the dominant factor-H-like protein 1 (FHL-1)- and factor-H-binding protein of Borrelia burgdorferi and is suggested to contribute to persistence of the pathogen. The prototype CRASP-1 of B. burgdorferi sensu stricto (CRASP-1Bb) has been formerly characterized. As shown recently, serum-resistant Borrelia afzelii strains express a unique FHL-1 and factor H-binding protein, designated CRASP-1Ba. Here, we describe for the first time the isolation and functional characterization of the gene encoding the full-length CRASP-1Ba of 28 kDa, which, upon processing, is predicted to be 26.4 kDa. CPASP-1Ba of B. afzelii spirochetes is associated with a genetic locus encoding the orthologous gbb54 gene family that maps to the linear plasmid of approximately 54 kb. Ligand affinity blotting techniques demonstrate that both native and recombinant CRASP-1Ba molecules strongly bind to FHL-1 and much more weakly to factor H. The FHL-1 and factor-H-binding site in CRASP-1Ba is shown to be localized to a 12-amino-acid residue domain at the C terminus of the protein. For comparison, the corresponding cspA-like gene(s) of a serum-sensitive Borrelia garinii strain has also been cloned and characterized. Most notably, two CRASP-1-related B. garinii proteins were identified; however, both molecules bind only weakly to FHL-1 and not at all to factor H. The present identification of the binding site of CRASP-1Ba represents an important step forward in our understanding of the pathogenesis of Lyme disease and may be helpful to design therapeutic regimens to interfere with complement evasion strategies of human pathogenic Borrelia strains.

Landscape Characterization of Peridomestic Risk for Lyme Disease Using Satellite Imagery

1997 ◽  
Vol 57 (6) ◽  
pp. 687-692 ◽  
Author(s):  
Sheri W. Dister ◽  
Durland Fish ◽  
Shannon M. Bros ◽  
Byron L. Wood ◽  
Denise H. Frank
Keyword(s):  
Lyme Disease ◽  
Satellite Imagery ◽  
Landscape Characterization

scholarly journals The Clinical, Symptom, and Quality-of-Life Characterization of a Well-Defined Group of Patients with Posttreatment Lyme Disease Syndrome

2017 ◽  
Vol 4 ◽  
Author(s):  
Alison W. Rebman ◽  
Kathleen T. Bechtold ◽  
Ting Yang ◽  
Erica A. Mihm ◽  
Mark J. Soloski ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Lyme Disease ◽  
Clinical Symptom

scholarly journals Identification of Novel Small RNAs and Characterization of the 6S RNA of Coxiella burnetii

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e100147 ◽  
Author(s):  
Indu Warrier ◽  
Linda D. Hicks ◽  
James M. Battisti ◽  
Rahul Raghavan ◽  
Michael F. Minnick
Keyword(s):  
Coxiella Burnetii ◽  
Small Rnas ◽  
6S Rna

Isolation and Morphological Characterization of Mosquito Spirochetes from a Lyme Disease Endemic Area

2006 ◽  
Vol 916 (1) ◽  
pp. 672-675 ◽  
Author(s):  
YIBAYIRI O. SANOGO ◽  
SIEGFRIED REIPERT ◽  
JIRÍ HALOUZKA ◽  
ZDENEK HUBÁLEK ◽  
MIROSLAV NEMEC
Keyword(s):  
Lyme Disease ◽  
Endemic Area ◽  
Morphological Characterization
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