scholarly journals Waking the neighbours: disruption of H-NS repression by overlapping transcription

2018 ◽  
Vol 108 (3) ◽  
pp. 221-225 ◽  
Author(s):  
Joseph T. Wade ◽  
David C. Grainger
Keyword(s):  
Overlapping Transcription

Overlapping transcription of the nifA regulatory gene in Rhizobium meliloti

Gene ◽  
1986 ◽  
Vol 50 (1-3) ◽  
pp. 141-148 ◽  
Author(s):  
Choong-Hyun Kim ◽  
Donald R. Helinski ◽  
Gary Ditta
Keyword(s):  
Rhizobium Meliloti ◽  
Regulatory Gene ◽  
Overlapping Transcription

Overlapping transcription structure of human cytomegalovirus UL140 and UL141 genes

2013 ◽  
Vol 38 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Yanping Ma ◽  
Mali Li ◽  
Bo Zheng ◽  
Ning Wang ◽  
Shuang Gao ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Overlapping Transcription

Overlapping transcription units in the dopa decarboxylase region of Drosophila

Nature ◽  
1986 ◽  
Vol 322 (6076) ◽  
pp. 279-281 ◽  
Author(s):  
Charlotte A. Spencer ◽  
R. Daniel Gietz ◽  
Ross B. Hodgetts
Keyword(s):  
Dopa Decarboxylase ◽  
Overlapping Transcription

scholarly journals Alternative Polyadenylation of Adeno-Associated Virus Type 5 RNA within an Internal Intron Is Governed by both a Downstream Element within the Intron 3′ Splice Acceptor and an Element Upstream of the P41 Initiation Site

2004 ◽  
Vol 78 (1) ◽  
pp. 83-93 ◽  
Author(s):  
Jianming Qiu ◽  
Ramnath Nayak ◽  
David J. Pintel
Keyword(s):  
Virus Type ◽  
Gene Promoter ◽  
Alternative Polyadenylation ◽  
Initiation Site ◽  
Splice Acceptor ◽  
Adeno Associated Virus ◽  
Multiple Promoters ◽  
Rep Proteins ◽  
Overlapping Transcription ◽  
A Site

ABSTRACT Adeno-associated virus type 5 (AAV5) has a linear, single-stranded DNA genome of ca. 5 kb and an overlapping transcription profile featuring multiple promoters and a single intron in the center of the genome. Unlike the situation for the prototype AAV2, AAV5 RNAs transcribed from upstream promoters at map units 7 (P7) and 19 (P19), which encode the viral Rep proteins, are predominantly polyadenylated at a site within the intron [(pA)p]. RNAs generated from the AAV5 capsid gene promoter P41, which is only 78 nucleotides (nt) upstream of the intron donor, and 281 nt upstream of (pA)p, primarily readthrough (pA)p, are polyadenylated at a more distal site at the 3′ end of the genome [(pA)d] and ultimately spliced. The intron contains the core sequences sufficient for polyadenylation at (pA)p, which is governed by a G/U-rich downstream element that overlaps with the intron 3′ A2 splice acceptor. In addition, polyadenylation of P7- and P19-generated RNAs at (pA)p is influenced by an upstream element that lies 5′ to the start of the P41 transcript. Our results also suggest that splicing and polyadenylation of P41-generated RNA can compete for the same pool of precursor pre-mRNA molecules. The cis-acting signals within the A2 3′ splice site that govern polyadenylation and splicing of AAV5 RNAs must be optimized to program both (i) the levels of polyadenylation of P7- and P19-generated RNA at (pA)p required to generate the proper levels of the essential Rep proteins and (ii) the splicing of P41-generated RNAs to generate the proper ratio of capsid proteins during AAV5 infection.

scholarly journals Conserved pattern of antisense overlapping transcription in the homologous human ERCC-1 and yeast RAD10 DNA repair gene regions.

1989 ◽  
Vol 9 (4) ◽  
pp. 1794-1798 ◽  
Author(s):  
M van Duin ◽  
J van Den Tol ◽  
J H Hoeijmakers ◽  
D Bootsma ◽  
I P Rupp ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Excision Repair ◽  
Antisense Transcription ◽  
Human Cells ◽  
Repair Gene ◽  
Naturally Occurring ◽  
Dna Repair Gene ◽  
Evolutionarily Conserved ◽  
Overlapping Transcription

We report that the genes for the homologous Saccharomyces cerevisiae RAD10 and human ERCC-1 DNA excision repair proteins harbor overlapping antisense transcription units in their 3' regions. Since naturally occurring antisense transcription is rare in S. cerevisiae and humans (this is the first example in human cells), our findings indicate that antisense transcription in the ERCC-1-RAD10 gene regions represents an evolutionarily conserved feature.

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