The phage tail tape measure protein, an inner membrane protein and a periplasmic chaperone play connected roles in the genome injection process ofE. coliphage HK97

2015 ◽  
Vol 96 (3) ◽  
pp. 437-447 ◽  
Author(s):  
Nichole Cumby ◽  
Kelly Reimer ◽  
Dominique Mengin-Lecreulx ◽  
Alan R. Davidson ◽  
Karen L. Maxwell
Keyword(s):  
Membrane Protein ◽  
Tape Measure Protein ◽  
Inner Membrane ◽  
Injection Process ◽  
Tape Measure ◽  
Phage Tail ◽  
Periplasmic Chaperone ◽  
Inner Membrane Protein

scholarly journals Genetic analysis of the role of the conserved inner membrane protein CvpA in EHEC resistance to deoxycholate

2020 ◽  
Author(s):  
Alyson R. Warr ◽  
Rachel T. Giorgio ◽  
Matthew K. Waldor
Keyword(s):  
Stress Response ◽  
Membrane Protein ◽  
Bile Salt ◽  
Cell Envelope ◽  
Enteric Bacteria ◽  
Enteric Pathogens ◽  
Inner Membrane ◽  
Bacterial Phyla ◽  
E Coli ◽  
Inner Membrane Protein

The function of cvpA, a bacterial gene predicted to encode an inner membrane protein, is largely unknown. Early studies in E. coli linked cvpA to Colicin V secretion and recent work revealed that it is required for robust intestinal colonization by diverse enteric pathogens. In enterohemorrhagic E. coli (EHEC), cvpA is required for resistance to the bile salt deoxycholate (DOC). Here, we carried out genome-scale transposon-insertion mutagenesis and spontaneous suppressor analysis to uncover cvpA’s genetic interactions and identify common pathways that rescue the sensitivity of a ΔcvpA EHEC mutant to DOC. These screens demonstrated that mutations predicted to activate the σE-mediated extracytoplasmic stress response bypass the ΔcvpA mutant’s susceptibility to DOC. Consistent with this idea, we found that deletions in rseA and msbB and direct overexpression of rpoE restored DOC resistance to the ΔcvpA mutant. Analysis of the distribution of CvpA homologs revealed that this inner membrane protein is conserved across diverse bacterial phyla, in both enteric and non-enteric bacteria that are not exposed to bile. Together, our findings suggest that CvpA plays a role in cell envelope homeostasis in response to DOC and similar stress stimuli in diverse bacterial species. IMPORTANCE Several enteric pathogens, including Enterohemorrhagic E. coli (EHEC), require CvpA to robustly colonize the intestine. This inner membrane protein is also important for secretion of a colicin and EHEC resistance to the bile salt deoxycholate (DOC), but its function is unknown. Genetic analyses carried out here showed that activation of the σE-mediated extracytoplasmic stress response restored the resistance of a cvpA mutant to DOC, suggesting that CvpA plays a role in cell envelope homeostasis. The conservation of CvpA across diverse bacterial phyla suggests that this membrane protein facilitates cell envelope homeostasis in response to varied cell envelope perturbations.

scholarly journals YieJ (CbrC) Mediates CreBC-Dependent Colicin E2 Tolerance in Escherichia coli

2010 ◽  
Vol 192 (13) ◽  
pp. 3329-3336 ◽  
Author(s):  
S. James L. Cariss ◽  
Chrystala Constantinidou ◽  
Mala D. Patel ◽  
Yuiko Takebayashi ◽  
Jon L. Hobman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Microarray Analysis ◽  
Gene Knockout ◽  
Inner Membrane ◽  
Two Component ◽  
Inner Membrane Protein ◽  
Colicin E2 ◽  
K 12

ABSTRACT Colicin E2-tolerant (known as Cet2) Escherichia coli K-12 mutants overproduce an inner membrane protein, CreD, which is believed to cause the Cet2 phenotype. Here, we show that overproduction of CreD in a Cet2 strain results from hyperactivation of the CreBC two-component regulator, but CreD overproduction is not responsible for the Cet2 phenotype. Through microarray analysis and gene knockout and overexpression studies, we show that overexpression of another CreBC-regulated gene, yieJ (also known as cbrC), causes the Cet2 phenotype.

scholarly journals Characterization of the two conformations adopted by the T3SS inner-membrane protein PrgK

2018 ◽  
Vol 27 (9) ◽  
pp. 1680-1691
Author(s):  
Julien R. C. Bergeron ◽  
Jacob A. Brockerman ◽  
Marija Vuckovic ◽  
Wanyin Deng ◽  
Mark Okon ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Inner Membrane ◽  
Inner Membrane Protein

scholarly journals Mitochondrial inner membrane protein, Mic60/mitofilin in mammalian organ protection

2018 ◽  
Vol 234 (4) ◽  
pp. 3383-3393 ◽  
Author(s):  
Yansheng Feng ◽  
Ngonidzashe B. Madungwe ◽  
Jean C. Bopassa
Keyword(s):  
Membrane Protein ◽  
Organ Protection ◽  
Inner Membrane ◽  
Mitochondrial Inner Membrane ◽  
Inner Membrane Protein
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