scholarly journals Characterization of the two conformations adopted by the T3SS inner-membrane protein PrgK

2018 ◽  
Vol 27 (9) ◽  
pp. 1680-1691
Author(s):  
Julien R. C. Bergeron ◽  
Jacob A. Brockerman ◽  
Marija Vuckovic ◽  
Wanyin Deng ◽  
Mark Okon ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Inner Membrane ◽  
Inner Membrane Protein

Biochemical characterization of the flagellar stator-associated inner membrane protein FliL fromVibrio alginolyticus

2016 ◽  
pp. mvw076 ◽  
Author(s):  
Ananthanarayanan Kumar ◽  
Miyu Isumi ◽  
Mayuko Sakuma ◽  
Shiwei Zhu ◽  
Yuuki Nishino ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Biochemical Characterization ◽  
Inner Membrane ◽  
Inner Membrane Protein

scholarly journals Characterization of the inner membrane protein BB0173 from Borrelia burgdorferi

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Christina M. Brock ◽  
Manuel Bañó-Polo ◽  
Maria J. Garcia-Murria ◽  
Ismael Mingarro ◽  
Maria Esteve-Gasent
Keyword(s):  
Membrane Protein ◽  
Borrelia Burgdorferi ◽  
Inner Membrane ◽  
Inner Membrane Protein

scholarly journals Characterization of the mitochondrial inner membrane protein translocator Tim17 from Trypanosoma brucei

2008 ◽  
Vol 159 (1) ◽  
pp. 30-43 ◽  
Author(s):  
Ujjal K. Singha ◽  
Emmanuel Peprah ◽  
Shuntae Williams ◽  
Robert Walker ◽  
Lipi Saha ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Trypanosoma Brucei ◽  
Inner Membrane ◽  
Mitochondrial Inner Membrane ◽  
Inner Membrane Protein

scholarly journals Genetic analysis of the role of the conserved inner membrane protein CvpA in EHEC resistance to deoxycholate

2020 ◽  
Author(s):  
Alyson R. Warr ◽  
Rachel T. Giorgio ◽  
Matthew K. Waldor
Keyword(s):  
Stress Response ◽  
Membrane Protein ◽  
Bile Salt ◽  
Cell Envelope ◽  
Enteric Bacteria ◽  
Enteric Pathogens ◽  
Inner Membrane ◽  
Bacterial Phyla ◽  
E Coli ◽  
Inner Membrane Protein

The function of cvpA, a bacterial gene predicted to encode an inner membrane protein, is largely unknown. Early studies in E. coli linked cvpA to Colicin V secretion and recent work revealed that it is required for robust intestinal colonization by diverse enteric pathogens. In enterohemorrhagic E. coli (EHEC), cvpA is required for resistance to the bile salt deoxycholate (DOC). Here, we carried out genome-scale transposon-insertion mutagenesis and spontaneous suppressor analysis to uncover cvpA’s genetic interactions and identify common pathways that rescue the sensitivity of a ΔcvpA EHEC mutant to DOC. These screens demonstrated that mutations predicted to activate the σE-mediated extracytoplasmic stress response bypass the ΔcvpA mutant’s susceptibility to DOC. Consistent with this idea, we found that deletions in rseA and msbB and direct overexpression of rpoE restored DOC resistance to the ΔcvpA mutant. Analysis of the distribution of CvpA homologs revealed that this inner membrane protein is conserved across diverse bacterial phyla, in both enteric and non-enteric bacteria that are not exposed to bile. Together, our findings suggest that CvpA plays a role in cell envelope homeostasis in response to DOC and similar stress stimuli in diverse bacterial species. IMPORTANCE Several enteric pathogens, including Enterohemorrhagic E. coli (EHEC), require CvpA to robustly colonize the intestine. This inner membrane protein is also important for secretion of a colicin and EHEC resistance to the bile salt deoxycholate (DOC), but its function is unknown. Genetic analyses carried out here showed that activation of the σE-mediated extracytoplasmic stress response restored the resistance of a cvpA mutant to DOC, suggesting that CvpA plays a role in cell envelope homeostasis. The conservation of CvpA across diverse bacterial phyla suggests that this membrane protein facilitates cell envelope homeostasis in response to varied cell envelope perturbations.

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