Guideline for in vivo assessment of adherent and rolling leukocytes in human skin microvasculature via reflectance confocal videomicroscopy

2021 ◽  
Author(s):  
Zijun Zhao ◽  
James Randall Patrinely ◽  
Inga Saknite ◽  
Michael Byrne ◽  
Eric R. Tkaczyk
Keyword(s):  
Human Skin ◽  
Rolling Leukocytes ◽  
In Vivo Assessment

In vitro and in vivo assessment of the irritation potential of different spin traps in human skin

Toxicology ◽  
2000 ◽  
Vol 151 (1-3) ◽  
pp. 55-63 ◽  
Author(s):  
Jürgen Fuchs ◽  
Norbert Groth ◽  
Thomas Herrling
Keyword(s):  
Human Skin ◽  
Spin Traps ◽  
In Vivo Assessment

In vivo assessment of aged human skin with a unilateral NMR scanner

2015 ◽  
Vol 28 (6) ◽  
pp. 656-666 ◽  
Author(s):  
Elad Bergman ◽  
Yifat Sarda ◽  
Noa Ritz ◽  
Edmond Sabo ◽  
Gil Navon ◽  
...  
Keyword(s):  
Human Skin ◽  
Unilateral Nmr ◽  
In Vivo Assessment

scholarly journals Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Evgeny A. Shirshin ◽  
Yury I. Gurfinkel ◽  
Alexander V. Priezzhev ◽  
Victor V. Fadeev ◽  
Juergen Lademann ◽  
...  
Keyword(s):  
Human Skin ◽  
Structural Proteins ◽  
Papillary Dermis ◽  
Blood Capillaries ◽  
Two Photon ◽  
Lifetime Imaging ◽  
In Vivo Assessment

Novel laser technologies for human skin in-vivo assessment

2007 ◽  
Author(s):  
Janis Spigulis ◽  
Alexey Lihachev ◽  
Lasma Gailite ◽  
Renars Erts
Keyword(s):  
Human Skin ◽  
Laser Technologies ◽  
In Vivo Assessment

In vivo assessment of safety of microneedle arrays in human skin

2008 ◽  
Vol 35 (3) ◽  
pp. 193-202 ◽  
Author(s):  
Suzanne M. Bal ◽  
Julia Caussin ◽  
Stan Pavel ◽  
Joke A. Bouwstra
Keyword(s):  
Human Skin ◽  
Microneedle Arrays ◽  
In Vivo Assessment

In vivo assessment of human skin aging by multiphoton laser scanning tomography

2006 ◽  
Vol 31 (19) ◽  
pp. 2879 ◽  
Author(s):  
Martin Johannes Koehler ◽  
Karsten König ◽  
Peter Elsner ◽  
Rainer Bückle ◽  
Martin Kaatz
Keyword(s):  
Human Skin ◽  
Laser Scanning ◽  
Skin Aging ◽  
In Vivo Assessment

scholarly journals In Vivo Assessment of Gene Delivery to Keratinocytes by Lentiviral Vectors

2002 ◽  
Vol 76 (3) ◽  
pp. 1496-1504 ◽  
Author(s):  
Ulrich Kuhn ◽  
Atsushi Terunuma ◽  
Wolfgang Pfutzner ◽  
Ruth Ann Foster ◽  
Jonathan C. Vogel
Keyword(s):  
Stem Cells ◽  
Progenitor Cells ◽  
Human Skin ◽  
Ex Vivo ◽  
Lentiviral Vectors ◽  
Retroviral Vectors ◽  
Immunocompromised Mice ◽  
In Vivo Assessment

ABSTRACT For skin gene therapy, introduction of a desired gene into keratinocyte progenitor or stem cells could overcome the problem of achieving persistent gene expression in a significant percentage of keratinocytes. Although keratinocyte stem cells have not yet been completely characterized and purified for gene targeting purposes, lentiviral vectors may be superior to retroviral vectors at gene introduction into these stem cells, which are believed to divide and cycle slowly. Our initial in vitro studies demonstrate that lentiviral vectors are able to efficiently transduce nondividing keratinocytes, unlike retroviral vectors, and do not require the lentiviral accessory genes for keratinocyte transduction. When lentiviral vectors expressing green fluorescent protein (GFP) were directly injected into the dermis of human skin grafted onto immunocompromised mice, transduction of dividing basal and nondividing suprabasal keratinocytes could be demonstrated, which was not the case when control retroviral vectors were used. However, flow cytometry analysis demonstrated low transduction efficiency, and histological analysis at later time points provided no evidence for progenitor cell targeting. In an alternative in vivo method, human keratinocytes were transduced in tissue culture (ex vivo) with either lentiviral or retroviral vectors and grafted as skin equivalents onto immunocompromised mice. GFP expression was analyzed in these human skin grafts after several cycles of epidermal turnover, and both the lentiviral and retroviral vector-transduced grafts had similar percentages of GFP-expressing keratinocytes. This ex vivo grafting study provides a good in vivo assessment of gene introduction into progenitor cells and suggests that lentiviral vectors are not necessarily superior to retroviral vectors at introducing genes into keratinocyte progenitor cells during in vitro culture.

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