Gene expression plasticity in response to salinity acclimation in threespine stickleback ecotypes from different salinity habitats

2017 ◽  
Vol 26 (10) ◽  
pp. 2711-2725 ◽  
Author(s):  
Taylor C. Gibbons ◽  
David C. H. Metzger ◽  
Timothy M. Healy ◽  
Patricia M. Schulte
Keyword(s):  
Gene Expression ◽  
Threespine Stickleback ◽  
Salinity Acclimation

scholarly journals Early Life Disruption of the Microbiota Affects Organ Development and Cytokine Gene Expression in Threespine Stickleback

2020 ◽  
Author(s):  
Lucas J Kirschman ◽  
Anastasia Khadjinova ◽  
Kelly Ireland ◽  
Kathryn C Milligan-Myhre
Keyword(s):  
Gene Expression ◽  
Early Life ◽  
Genetic Background ◽  
Gasterosteus Aculeatus ◽  
Threespine Stickleback ◽  
Microbial Colonization ◽  
Cytokine Gene Expression ◽  
Immune Gene ◽  
Cytokine Gene

Synopsis The microbiota that inhabits vertebrates exerts strong effects on host physiology and can be crucial to the development of a normal phenotype. This includes development of the immune system, somatic growth and maintenance, and morphogenesis. However, the genetic background of the host can also affect these life history traits. To this end, we investigated the effects of the microbiota on growth, development, and immune gene expression on two populations of threespine stickleback (Gasterosteus aculeatus), one anadromous and one freshwater. We tested the hypotheses that microbial colonization and the genetic background of the host would affect survival, cytokine gene expression, growth, and development. We raised in vitro crosses of stickleback larvae with and without conventional microbiota. We then exposed all these treatments to Vibrio anguillarum, a potential fish pathogen, in a full factorial design. We found stickleback raised without conventional microbiota had smaller swim bladders relative to those raised with conventional microbiota. Stickleback raised with conventional microbiota exhibited small increases in cytokine gene expression. We found no differences in growth or survival regardless of treatment. These results are consistent with other investigations that show microbiota disruption, in early life, can alter host organ and tissue development and immune responses

Gene expression plasticity evolves in response to colonization of freshwater lakes in threespine stickleback

2014 ◽  
Vol 23 (13) ◽  
pp. 3226-3240 ◽  
Author(s):  
Matthew R. J. Morris ◽  
Romain Richard ◽  
Erica H. Leder ◽  
Rowan D. H. Barrett ◽  
Nadia Aubin-Horth ◽  
...  
Keyword(s):  
Gene Expression ◽  
Threespine Stickleback ◽  
Freshwater Lakes

scholarly journals Regulatory Architecture of Gene Expression Variation in the Threespine Stickleback Gasterosteus aculeatus

2016 ◽  
Vol 7 (1) ◽  
pp. 165-178 ◽  
Author(s):  
Victoria L. Pritchard ◽  
Heidi M. Viitaniemi ◽  
R. J. Scott McCairns ◽  
Juha Merilä ◽  
Mikko Nikinmaa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gasterosteus Aculeatus ◽  
Threespine Stickleback ◽  
Gene Expression Variation ◽  
Expression Variation ◽  
Regulatory Architecture

scholarly journals Influence of Salinity on the Survival and Growth of Juvenile Coregonus Ussuriensis Berg

2021 ◽  
Author(s):  
Tianqing Huang ◽  
Enhui Liu ◽  
Wei Gu ◽  
Bingqian Wang ◽  
Fulin Dong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Serum Cortisol ◽  
Physiological Adaptation ◽  
Estuarine Fish ◽  
Salinity Range ◽  
Salinity Change ◽  
Salinity Acclimation ◽  
Blood Biochemical ◽  
Significant Difference ◽  
In Captivity

Abstract To explore the suitable salinity range of Coregonus ussuriensis Berg, we investigated the effect of induced salinity change in captivity on C. ussuriensis with an initial body weight of 35 ± 1.5 g. After 30 days of salinity acclimation, the survival, growth performance, blood biochemical profiles, antioxidative capacity, and tissue structure of juveniles under four salinity conditions (8‰, 16‰, 24‰, and 32‰) were investigated. Our results revealed that serum penetration, blood glucose, and serum Na+, Cl−, and Mg2+ gradually increased with increasing salinity until 32‰ salinity, when a significant difference was observed, whereas the K+ concentration showed a downward trend. The tissue sections showed that under high salinity (32‰), the liver and gill tissues of the fish were severely damaged and the vacuolation was serious. The levels of superoxide dismutase, glutathione peroxidase, and serum cortisol gradually increased with increasing salinity. A gene expression analysis showed that the increase in salinity induced higher expression of stress-, growth-, and inflammation-related genes (HSP70, Gh and Igf-1, and IL-1β, respectively). The downregulation of stress-related gene expression at 32‰ salinity may indicate that this level of salinity exceeded the regulatory capacity of C. ussuriensis. We concluded that C. ussuriensis may survive in an estuary under 0–24‰ salinity. Our findings provide insights into the physiological adaptation of C. ussuriensis to salinity change. These results could improve our knowledge of the stress response and resilience of estuarine fish to hyposalinity and hypersalinity stress.

scholarly journals Between‐population differences in constitutive and infection‐induced gene expression in threespine stickleback

2021 ◽  
Author(s):  
Lauren E. Fuess ◽  
Jesse N. Weber ◽  
Stijn den Haan ◽  
Natalie C. Steinel ◽  
Kum Chuan Shim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Threespine Stickleback ◽  
Population Differences

Dynamics of Gene Expression Responses for Ion Transport Proteins and Aquaporins in the Gill of a Euryhaline Pupfish during Freshwater and High-Salinity Acclimation

2018 ◽  
Vol 91 (6) ◽  
pp. 1148-1171 ◽  
Author(s):  
Sean C. Lema ◽  
Paul G. Carvalho ◽  
Jennifer N. Egelston ◽  
John T. Kelly ◽  
Stephen D. McCormick
Keyword(s):  
Gene Expression ◽  
Ion Transport ◽  
High Salinity ◽  
Transport Proteins ◽  
Salinity Acclimation

scholarly journals Gene expression responses of threespine stickleback to salinity: implications for salt-sensitive hypertension

2014 ◽  
Vol 5 ◽  
Author(s):  
Gang Wang ◽  
Ence Yang ◽  
Kerri J. Smith ◽  
Yong Zeng ◽  
Guoli Ji ◽  
...  
Keyword(s):  
Gene Expression ◽  
Threespine Stickleback ◽  
Salt Sensitive Hypertension

scholarly journals Regulatory architecture of gene expression variation in the threespine stickleback, Gasterosteus aculeatus

2016 ◽  
Author(s):  
Victoria L. Pritchard ◽  
Heidi M. Viitaniemi ◽  
R.J. Scott McCairns ◽  
Juha Merilä ◽  
Mikko Nikinmaa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Regulatory Networks ◽  
Gasterosteus Aculeatus ◽  
Genotyping By Sequencing ◽  
Interval Mapping ◽  
Threespine Stickleback ◽  
Adaptive Divergence ◽  
Gene Expression Variation ◽  
Expression Variation ◽  
Regulatory Architecture

Much adaptive evolutionary change is underlain by mutational variation in regions of the genome that regulate gene expression rather than in the coding regions of the genes themselves. An understanding of the role of gene expression variation in facilitating local adaptation will be aided by an understanding of underlying regulatory networks. Here, we characterize the genetic architecture of gene expression variation in the threespine stickleback (Gasterosteus aculeatus), an important model in the study of adaptive evolution. We collected transcriptomic and genomic data from 60 half-sib families using an expression microarray and genotyping-by-sequencing, and located QTL underlying the variation in gene expression (eQTL) in liver tissue using an interval mapping approach. We identified eQTL for several thousand expression traits. Expression was influenced by polymorphism in both cis and trans regulatory regions. Trans eQTL clustered into hotspots. We did not identify master transcriptional regulators in hotspot locations: rather, the presence of hotspots may be driven by complex interactions between multiple transcription factors. One observed hotspot co-located with a QTL recently found to underlie salinity tolerance in the threespine stickleback. However, most other observed hotspots did not co-locate with regions of the genome known to be involved in adaptive divergence between marine and freshwater habitats.

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