scholarly journals Hypoxia‐inducible factor 2α drives hepatosteatosis through the fatty acid translocase CD36

2020 ◽  
Vol 40 (10) ◽  
pp. 2553-2567 ◽  
Author(s):  
Esther Rey ◽  
Florinda Meléndez‐Rodríguez ◽  
Patricia Marañón ◽  
Miriam Gil‐Valle ◽  
Almudena G. Carrasco ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Hypoxia Inducible Factor ◽  
Fatty Acid Translocase

FRI-298-Hypoxia-inducible factor 2 alpha drives lipid accumulation in human hepatocytes through the fatty acid translocase CD36

2019 ◽  
Vol 70 (1) ◽  
pp. e526
Author(s):  
Carmelo García-Monzón ◽  
Esther Rey ◽  
Florinda Meléndez-Rodríguez ◽  
Patricia Marañón ◽  
Elvira Del Pozo-Maroto ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Accumulation ◽  
Hypoxia Inducible Factor ◽  
Human Hepatocytes ◽  
Fatty Acid Translocase

scholarly journals A sensitive immunoassay for rat fatty acid translocase (CD36) using phage antibodies selected on cell transfectants: abundant presence of fatty acid translocase/CD36 in cardiac and red skeletal muscle and up-regulation in diabetes

1999 ◽  
Vol 337 (3) ◽  
pp. 407-414 ◽  
Author(s):  
Maurice M. A. L. PELSERS ◽  
Jan T. LUTGERINK ◽  
Frans A. van NIEUWENHOVEN ◽  
Narendra N. TANDON ◽  
Ger J. van der VUSSE ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Membrane Protein ◽  
Diabetic Rats ◽  
Direct Selection ◽  
Antibody Library ◽  
Fatty Acid Binding ◽  
Fatty Acid Translocase ◽  
Phage Antibody ◽  
Phage Antibodies ◽  
Set Up

The rat membrane protein fatty acid translocase (FAT), which shows sequence similarity to human CD36 (a membrane protein supposedly involved in a variety of membrane processes), is implicated in the transport of long-chain fatty acids across cellular membranes. To set up an immunoassay for quantification of FAT in different tissues, we isolated a series of anti-FAT antibodies by panning a large naive phage antibody library on FAT-transfected H9c2 cells. All seven different phage antibody fragments isolated reacted specifically with FAT, and most likely recognize the same or closely located immunodominant sites on FAT, as a competitive monoclonal antibody (mAb) (CLB-IV7) completely blocked the binding of all these phage antibodies to cells. A sandwich ELISA was set up using mAb 131.4 (directed against purified CD36 from human platelets) as capture antibody and phage antibodies and anti-phage sera as detector. With this ELISA (sensitivity 0.05 µg/ml), the FAT content in isolated cardiomyocytes was found to be comparable with that of total heart (≈ 3 mg/g of protein), while liver tissue and endothelial cells were below the detection limit (< 0.1 mg of FAT/g of protein). During rat heart development, protein levels of FAT rose from 1.7±0.7 mg/g of protein on the day before birth to 3.6±0.4 mg/g of protein on day 70. Comparing control with streptozotocin-induced diabetic rats, a statistically significant (P< 0.05) 2–4-fold increase of FAT was seen in heart (from 4.2±2.3 to 11.0±5.7 mg/g of protein), soleus (from 0.6±0.1 to 1.4±0.5 mg/g of protein) and extensor digitorum longus (EDL) muscle (from 0.3±0.1 to 1.2±0.8 mg/g of protein). In addition, the FAT contents of each of these muscles were found to be of similar magnitude to the contents of cytoplasmic heart-type fatty-acid-binding protein in both diabetic rats and controls, supporting the suggested roles of these two proteins in cellular fatty acid metabolism. This is the first time phage display technology has been succesfully applied for direct selection, from a large naive antibody library, of antibodies that recognize selected membrane proteins in their natural context.

scholarly journals Abstract 2880: CD36, a fatty acid translocase, promotes metastasis in CRC

2021 ◽  
Author(s):  
James Michael Drury ◽  
Piotr Rychahou ◽  
Heidi L. Weiss ◽  
Yekaterina Y. Zaytseva
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Translocase

The impact of overexpression and deficiency of fatty acid translocase (FAT)/CD36

2002 ◽  
pp. 193-197
Author(s):  
M. L. Febbraio ◽  
E. Guy ◽  
C. Coburn ◽  
F. F. Knapp ◽  
A. L. Beets ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Translocase ◽  
The Impact

Activation of AMPK by berberine induces hepatic lipid accumulation by upregulation of fatty acid translocase CD36 in mice

2017 ◽  
Vol 316 ◽  
pp. 74-82 ◽  
Author(s):  
You-Jin Choi ◽  
Kang-Yo Lee ◽  
Seung-Hwan Jung ◽  
Hyung Sik Kim ◽  
Gayong Shim ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Accumulation ◽  
Fatty Acid Translocase ◽  
Hepatic Lipid ◽  
Hepatic Lipid Accumulation

scholarly journals A Novel Function for Fatty Acid Translocase (FAT)/CD36

2004 ◽  
Vol 279 (35) ◽  
pp. 36235-36241 ◽  
Author(s):  
Shannon E. Campbell ◽  
Narendra N. Tandon ◽  
Gebretateos Woldegiorgis ◽  
Joost J. F. P. Luiken ◽  
Jan F. C. Glatz ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Translocase

scholarly journals Intrahepatic Expression of Fatty Acid Translocase CD36 Is Increased in Obstructive Sleep Apnea

2020 ◽  
Vol 7 ◽  
Author(s):  
Esther Rey ◽  
Elvira del Pozo-Maroto ◽  
Patricia Marañón ◽  
Brittany Beeler ◽  
Yaiza García-García ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Fatty Acid ◽  
Sleep Apnea ◽  
Fatty Acid Translocase ◽  
Obstructive Sleep

scholarly journals Fatty Acid Translocase (FAT/CD36) Is Localized on Insulin-Containing Granules in Human Pancreatic  -Cells and Mediates Fatty Acid Effects on Insulin Secretion

Diabetes ◽  
2005 ◽  
Vol 54 (2) ◽  
pp. 472-481 ◽  
Author(s):  
H. Noushmehr ◽  
E. D'Amico ◽  
L. Farilla ◽  
H. Hui ◽  
K. A. Wawrowsky ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Fatty Acid ◽  
Fatty Acid Translocase ◽  
Pancreatic Cells
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