Methylene blue active substances in plaque of Bacillus subtilis subsp. subtilis and enrichment by supplemental calcium in culture media

2020 ◽  
Vol 71 (5) ◽  
pp. 550-556
Author(s):  
L.A. Krueger ◽  
M.A. Grzemski ◽  
M.C. Bilyeu ◽  
J.G. Horst ◽  
S.A. Ugrin ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Bacillus Subtilis ◽  
Culture Media ◽  
Supplemental Calcium ◽  
Subtilis Subsp ◽  
Active Substances

scholarly journals Complete Genome Sequence of Bacillus subtilis subsp. subtilis Strain ∆6

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Daniel R. Reuß ◽  
Andrea Thürmer ◽  
Rolf Daniel ◽  
Wim J. Quax ◽  
Jörg Stülke
Keyword(s):  
Bacillus Subtilis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Full Genome Sequence ◽  
Subtilis Strain ◽  
Full Genome ◽  
Biotechnological Applications ◽  
A Genome ◽  
Subtilis Subsp

Bacillus subtilis ∆6 is a genome-reduced strain that was cured from six prophages and AT-rich islands. This strain is of great interest for biotechnological applications. Here, we announce the full-genome sequence of this strain. Interestingly, the conjugative element ICE Bs 1 has most likely undergone self-excision in B. subtilis ∆6.

Anionic polymers of the cell wall of Bacillus subtilis subsp. subtilis VKM B-501T

2009 ◽  
Vol 74 (5) ◽  
pp. 543-548 ◽  
Author(s):  
A. S. Shashkov ◽  
N. V. Potekhina ◽  
S. N. Senchenkova ◽  
E. B. Kudryashova
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Anionic Polymers ◽  
Subtilis Subsp

Overexpression of hemA and hemL in Bacillus subtilis Promotes Overexpression of 5-aminolevulinic Acid 

2020 ◽  
Author(s):  
Jinni Liu ◽  
Zhaowei Ye ◽  
Haigang Wu ◽  
Jicheng Liu ◽  
Yuesheng Gong
Keyword(s):  
Bacillus Subtilis ◽  
Acid Content ◽  
Soluble Protein ◽  
Aminolevulinic Acid ◽  
Culture Media ◽  
Total Soluble Protein ◽  
Target Proteins

Bacillus subtilis strains were constructed that overexpressed the glutamyl t-RNA reductase (HemA) and glutamyl t-RNA synthase (HemL). The target proteins accounted for 11 and 13% of the total soluble protein in maltose induced extracts, respectively. The culture media from these strains was red with wavelength maxima near 400 nm indicating elevated levels of porphyrins. The 5-aminolevulinic acid content for these strains reached 42.57 and 39.78 mg/L after 24 h of induced growth and 65.23 and 68.45 mg/L, respectively, after 60 h. Therefore, hemA and hemL overexpression enhanced the biosynthesis of 5-aminolevulinic acid and these strains can be used for its purification.

scholarly journals Microbiological quality of commercially available poultry feeds sold in Bangladesh

2017 ◽  
Vol 3 (1) ◽  
pp. 52-60
Author(s):  
Nigar Sultana ◽  
Md Atiqul Haque ◽  
Md Mostafizer Rahman ◽  
Mir Rowshan Akter ◽  
Mst Deloara Begum ◽  
...  
Keyword(s):  
Public Health ◽  
Methylene Blue ◽  
Culture Media ◽  
Microbiological Quality ◽  
Control Measures ◽  
Nutrient Agar ◽  
Poultry Feed ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
E Coli

The study was conducted aiming at the isolation and identification of pathogens from poultry feed manufactured by four different brands namely A (broiler starter), B (broiler finisher), C (layer starter) and D (layer finisher) sold in retail stores of Rangpur city of Bangladesh. All these samples were collected from four randomly chosen outlets and analyzed by culturing in different culture media such as Nutrient broth (NB), Nutrient agar (NA), Salmonella-Shigella (SS) agar, Eosin methylene blue (EMB) agar, MacConkey agar, Triple sugar iron (TSI) agar slant, Motility, Indole, Urease (MIU) and Saboraud Dextrose agar (SDA) media. The bacterial agents were isolated and examined under light microscope for their gross morphological and conventional biochemical characteristics. The bacteriological analyses were done at the Microbiology Laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur during the period of January to June, 2014. Total bacterial colonies of all the samples were counted separately according to the American Public Health Association, using nutrient agar medium for total viable count (TVC), Eosine methylene blue (EMB) agar media for total E. coli count (TEC) and Salmonella-Shigella agar for TSC (total salmonella count). Saboraud Dextrose agar (SDA) media was used for detection of fungus. The virulence effect of the organism present in feed were observed by inoculating the organism in poultry. Recorded result showed that average TVC of feed sample A, B, C and D were 5.45x106, 3.28x105, 5.14x106 and 4.53x105 CFU/gm (colony forming unit per gram) respectively. TEC of feed sample A, B, C and D were recorded 6.25x105, 8.26x103, 5.52x105 and 5.65x104 CFU/gm respectively. TSC of feed sample A, B, C and D were recorded 3.15x104, 2.68x103, 4.46x103 and 1.19x104 CFU/gm respectively. The highest TVC, TEC and TSC were found in broiler starter (feed sample A) and lowest TVC, TEC and TSC were found in broiler finisher (feed sample B). Fungal count was 1.85x105 CFU/ gm in layer finisher (feed sample D) could be as a result of their high pathogenecity as reported by researchers elsewhere. These organisms can cause several poultry and farm animal infections specially mycotoxicosis having public health significance to both human and poultry. The presence of high numbers of E. coli and Salmonella spp. in poultry feed were indicative of poor hygienic practices during manufacture, post process contamination and unsatisfactory transportation and reservation. Therefore reinforce the need for preventive control measures, hygienic handling and processing of feeds to reduce the risk of potential human health hazards.Asian J. Med. Biol. Res. March 2017, 3(1): 52-60

scholarly journals Isolation and Optimal Fermentation Condition of the Bacillus subtilis Subsp. natto Strain WTC016 for Nattokinase Production

Fermentation ◽  
2019 ◽  
Vol 5 (4) ◽  
pp. 92 ◽  
Author(s):  
Shouyong Ju ◽  
Zhilin Cao ◽  
Christina Wong ◽  
Yangyang Liu ◽  
Mohamed F. Foda ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Bacillus Subtilis ◽  
Fibrinolytic Activity ◽  
Inoculum Size ◽  
Orthogonal Test ◽  
Fermentation Condition ◽  
Fermentation Conditions ◽  
Optimal Tests ◽  
Physiological Tests ◽  
Subtilis Subsp

Nattokinase is a serine protease in the subtilisin family which is produced by Bacillus subtilis subsp. natto and exhibits vigorous fibrinolytic activity that has been suggested to be able to prevent and treat thromboembolic diseases. In this study, WTC016, a spore-forming and rod-shaped bacterium with fibrinolytic activity was successfully isolated from soil, which was identified as Bacillus subtilis subsp. natto based on morphological and physiological tests, and phylogenetic analysis of 16S rRNA and gyrA. According to the growth curve of WTC016, the nattokinase production reached the highest amount in the stationary phase. To optimize the liquid fermentation condition for nattokinase yield of WTC016, further optimal tests of four factors, including the temperature, pH, inoculum size, and loading volume, followed by orthogonal test of all these factors, was performed. The optimal fermentation conditions were determined as 30 °C, 7.0 pH, 2% inoculum size, and 60 mL of loading volume in 250 mL conical flask, which indicates the highest nattokinase production of 3284 ± 58 IU/mL while fermented for 26 h. This work laid the foundation for producing nattokinase using Bacillus subtilis subsp. natto WTC016.

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