Genetic variation in Pythium myriotylum based on SNP typing and development of a PCR-RFLP detection of isolates recovered from Pythium soft rot ginger

2017 ◽  
Vol 65 (4) ◽  
pp. 319-326 ◽  
Author(s):  
D.P. Le ◽  
M.K. Smith ◽  
E.A.B. Aitken
Keyword(s):  
Genetic Variation ◽  
Soft Rot ◽  
Pythium Myriotylum ◽  
Snp Typing ◽  
Pcr Rflp ◽  
Pythium Soft Rot

Pythium myriotylum is recovered most frequently from Pythium soft-rot infected ginger rhizomes in China

Plant Disease ◽  
2021 ◽  
Author(s):  
Paul Daly ◽  
Yifan Chen ◽  
Qimeng Zhang ◽  
Hongli Zhu ◽  
Jingjing Li ◽  
...  
Keyword(s):  
Severe Disease ◽  
Zingiber Officinale ◽  
Soft Rot ◽  
Temperature Dependent ◽  
Pythium Myriotylum ◽  
Disease Symptoms ◽  
Ginger Rhizome ◽  
Oomycete Pathogen ◽  
Dependent Growth ◽  
Pythium Soft Rot

Pythium soft rot is a major soil-borne disease of crops such as ginger (Zingiber officinale). Our objective was to identify which Pythium species were associated with Pythium soft-rot of ginger in China, where approximately 20% of global ginger production is from. Oomycetes infecting ginger rhizomes from seven provinces were investigated using two molecular markers, the internal transcribed spacer (ITS) and cytochrome c oxidase subunit II (CoxII). In total, 81 isolates were recovered and approximately 95% of the isolates were identified as Pythium myriotylum and the other isolates were identified as either P. aphanidermatum or P. graminicola. Notably, the P. myriotylum isolates from China did not contain the SNP in the CoxII sequence found previously in the P. myriotylum isolates infecting ginger in Australia. A subset of 36 of the isolates was analyzed repeatedly by temperature-dependent growth, severity of disease on ginger plants and aggressiveness of colonization of ginger rhizome sticks. In the pathogenicity assays, 32/36 of the isolates were able to significantly infect and cause severe disease symptoms on the ginger plants. A range of temperature-dependent growth, disease severity and aggressiveness in colonization was found with a significant moderate positive correlation between growth and aggressiveness of colonization of the ginger sticks. This study identified P. myriotylum as the major oomycete pathogen in China from infected ginger rhizomes and suggests that P. myriotylum should be a key target to control soft rot of ginger disease.

scholarly journals Genetic variation among pathogens causing "Helminthosporium" diseases of rice, maize and wheat

2002 ◽  
Vol 27 (6) ◽  
pp. 639-643 ◽  
Author(s):  
RITA C. B. WEIKERT-OLIVEIRA ◽  
M. APARECIDA DE RESENDE ◽  
HENRIQUE M. VALÉRIO ◽  
RACHEL B. CALIGIORNE ◽  
EDILSON PAIVA
Keyword(s):  
Triticum Aestivum ◽  
Genetic Variation ◽  
Climatic Factors ◽  
Rapd Analysis ◽  
Its Region ◽  
Restriction Enzymes ◽  
Fungal Species ◽  
High Similarity ◽  
Pcr Rflp ◽  
Upgma Phenogram

Twenty isolates of four fungal species, agents of "Helminthosporium" diseases in cereals, were collected from different regions: nine Bipolarisoryzae isolated from rice (Oryza sativa), seven B.sorokiniana from wheat (Triticum aestivum), two B. maydis, and two Exserohilumturcicum from maize (Zea mays). The strains were compared by PCR-RFLP and RAPD analysis. Size polymorphism among the isolates in the ITS region comprising the 5.8 S rDNA indicated genetic differences among the isolates, while a UPGMA phenogram constructed after the digestion of this region with restriction enzymes showed inter- and intra-specific polymorphism. The RAPD profiles indicated an expressive level of polymorphism among different species, compared with a low level of polymorphism among isolates of the same species. A UPGMA phenogram grouped the isolates according to the species and their host plant. RAPD profiles did not reveal polymorphism that directly correlated climatic factors with geographic source of the isolates of B. sorokiniana, and B. oryzae. Teleomorphic species revealed high similarity with their correspondent anamorphs.

scholarly journals Genetic polymorphism of candidate genes in pig meat production

2011 ◽  
pp. 37-40
Author(s):  
Zuzana Lieskovská ◽  
Anton Kováčik ◽  
Anna Trakovická
Keyword(s):  
Genetic Variation ◽  
Genetic Polymorphism ◽  
Candidate Genes ◽  
Fat Content ◽  
Meat Production ◽  
Lepr Gene ◽  
Pcr Rflp ◽  
Pig Meat

H-FABP, LEPR and MC5R genes were suggested as candidate genes for fat content in pig meat. The aim of this study was to detect genetic variation in the porcine H-FABP, LEPR and MC5R genes by PCR-RFLP method in a group of pigs. Genotyping of pigs was done by PCRRFLP methods. We identified three genotypes in the set of pigs, HH (0.504), Hh (0.412) and hh (0.084) for H-FABP (HinfI). Allele H showed higher frequency than allele h (0.710 vs. 0.290). Three genotypes were identified for the H-FABP (HaeIII) gene (DD - 0.194, Dd - 0.494, dd - 0.312). The allele D (0.441) showed slightly lower frequency than allele d (0.559). All three genotypes were identified for LEPR (HpaII) in the group of pigs (AA – 0.137, AB - 0.314, BB – 0.549). Higher frequency of LEPR gene was confirmed for allele B (0.706), as compared with allele A (0.294). We identified two genotypes for MC5R (BsaHI) in the group of pigs (AA - 0.348 and AG - 0.652), genotype GG was not found. As conforms with genotype structure, we recognize a higher frequency of allele A (0.674) as compared with allele G (0.326). 

Currutaca: A Pythium Soft Rot of Xanthosoma and Colocasia spp. in Puerto Rico

1969 ◽  
Vol 55 (1) ◽  
pp. 78-84
Author(s):  
L. A. Alvarez-García ◽  
A. Cortés-Monllor
Keyword(s):  
Puerto Rico ◽  
Soft Rot ◽  
Pythium Ultimum ◽  
Soft Rot Disease ◽  
Susceptibility To Infection ◽  
Percent Relative Humidity ◽  
Agar Media ◽  
Clean Seed ◽  
Rot Disease ◽  
Pythium Soft Rot

The fungus Pythium ultimum Trow, was found the causal agent of a soft rot disease of taniers [Xanthosoma sagittaefolium (L.) Schott.] and taros [Colocasia esculenta (L.) Schott.]. A yellow pigmented bacterium occasionally associated with the rot turned out to be a saprophyte. Roots of diseased plants became mucilaginous and decayed rapidly. The infected rhizomes were soft and cheesy. The base of infected petioles were water-soaked and the leaves collapsed. The fungus was isolated in pure culture and grew well in most culture agar media, producing abundant sporangia at 20-30° C., but failed to grow at temperatures below 12° C. or above 36° C. Varieties of taniers and taro inoculated with P. ultimum showed differential degrees of susceptibility to infection when kept in an environment of 90-percent relative humidity and a temperature of 20° C. Definitive measures to control the disease effectively are not known. Use of clean "seed", planting in well drained soils, and crop rotation practices, however, have been recommended.

PCR-RFLP Polymorphism of Growth Hormone Gene and Its Association with Growth and Morphometric Traits in Ganjam and Baigani Goats of Odisha

2020 ◽  
Author(s):  
D. K. Karna ◽  
A. Aradhana ◽  
G. D. Nayak ◽  
N. Sahoo ◽  
L. K. Sahoo ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Genetic Variation ◽  
Restriction Enzyme ◽  
Gene Segment ◽  
Growth Hormone Gene ◽  
Morphometric Traits ◽  
Exon 2 ◽  
Genotype Frequencies ◽  
Significant Difference ◽  
Pcr Rflp

Background: Growth Hormone (GH) gene codes for the growth hormone, an anabolic hormone synthesized and secreted by the somatotroph cells of pituitary. Growth hormone influences many processes such as growth, lactation, reproduction and metabolism. Genetic variation in the gene are utilized as markers for selecting animals that are superior in terms growth, production and reproduction traits. Information available on the polymorphism of growth hormone gene of Ganjam and Baigani goats of Odisha is scanty. The current investigation was done to explore the genetic variation of this gene and its association with morphometric traits. Methods: Genetic polymorphism in exon 2 and 3 of Growth hormone gene in Ganjam goat and Baigani goat was explored with sample size of 100 goats for each. The goats belonged to three locations: Khallikote, Rambha, Chattrapur of Ganjam district. The goats were recorded for their body weights, morphometric traits and morphological traits. Genomic DNA was isolated, the target segment comprising exons 2 and 3 was amplified and PCR-RFLP was carried out using Hae III restriction enzyme. Genotypes were scored. Result: PCR of the locus resulted in 422 bp PCR product. PCR-RFLP using Hae III restriction enzyme yielded only two variants in both the populations. The variant A had only one restriction recognition site on the target gene segment yielding two bands with size of 366bp and 56bp whereas the variant B did not have any restriction site with single band of 422bp. Three genotypes AA, AB and BB were found in both the population. In both the population, the gene and genotype frequency were significantly deviated from the Hardy Weinberg Equilibrium frequency. There was a significant difference in the genotype frequencies of growth hormone gene between Ganjam and Baigani goats. The AB genotype had higher mean value for all morphometric traits than AA and BB genotype though the differences were not found to be significant.

A DdeI PCR-RFLP detecting genetic variation of goat POU1F1 gene

2007 ◽  
Vol 87 (1) ◽  
pp. 13-14 ◽  
Author(s):  
X. Y. Lan ◽  
C. Y. Pan ◽  
H. Chen ◽  
C. Z. Lei
Keyword(s):  
Genetic Variation ◽  
Gene Polymorphism ◽  
Key Words ◽  
Milk Yield ◽  
Dairy Goat ◽  
Pou1f1 Gene ◽  
Pcr Rflp ◽  
Silent Allele

We described a DdeI PCR-RFLP method for detecting silent allele at goat POU1F1 locus: p.S241S. Frequencies of D1 allele varied from 0.600 to 1.000 in eight Chinese native breeds. Association of DdeI RFLP genotypes with milk yield of dairy goat was significant (P < 0.05). Key words: Goat, POU1F1 gene, polymorphism, association, milk yield

scholarly journals Genetic Variation of Calpastation Gene of Indigenous Bali Cattle (Bos javanicus) in Indonesia

2019 ◽  
Vol 26 (1) ◽  
pp. 44
Author(s):  
Endang Tri Margawati ◽  
Slamet Diah Volkandari ◽  
Indriawati Indriawati ◽  
Emma M. Svensson
Keyword(s):  
Genetic Variation ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Meat Tenderness ◽  
Gene Markers ◽  
Bos Javanicus ◽  
Restriction Fragment Polymorphism ◽  
Pcr Rflp ◽  
Bali Cattle ◽  
Cast Gene

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.

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