scholarly journals mcrAGene abundance correlates with hydrogenotrophic methane production rates in full-scale anaerobic waste treatment systems

2015 ◽  
Vol 62 (2) ◽  
pp. 111-118 ◽  
Author(s):  
R.L. Morris ◽  
V.P. Tale ◽  
P.P. Mathai ◽  
D.H. Zitomer ◽  
J.S. Maki
1989 ◽  
Vol 21 (10-11) ◽  
pp. 1389-1402 ◽  
Author(s):  
R. Zaloum

Deviations from design expectations appear to stem from views which assume that a unique response should result from a given set of operating conditions. The results of this study showed that two systems operating at equal organic loads or F/M ratios and at the same SRT do not necessarily give equal responses. This deviation was linked to the manner in which the HRT and influent COD are manipulated to obtain a constant or uniform load, and to subtle interactions between influent COD, HRT and SRT on the biomass and effluent responses. Increases of up to 200% in influent COD from one steady level to the next did not significantly influence the effluent VSS concentration while an effect on filtered COD was observed for increases as low as 20%. Effluent TKN and filtered COD correlated strongly with the operating MLVSS while phosphorus residual depended on the operating SRT and the organic load removed. These results point to the inadequacy of traditional models to predict effluent quality and point to the need to consider these effects when developing simulation techniques or computer assisted expert systems for the control of waste treatment plants.


2017 ◽  
Vol 43 (3) ◽  
pp. 53-60 ◽  
Author(s):  
Piotr Świątczak ◽  
Agnieszka Cydzik-Kwiatkowska ◽  
Paulina Rusanowska

AbstractAnaerobic digestion is an important technology for the bio-based economy. The stability of the process is crucial for its successful implementation and depends on the structure and functional stability of the microbial community. In this study, the total microbial community was analyzed during mesophilic fermentation of sewage sludge in full-scale digesters.The digesters operated at 34–35°C, and a mixture of primary and excess sludge at a ratio of 2:1 was added to the digesters at 550 m3/d, for a sludge load of 0.054 m3/(m3·d). The amount and composition of biogas were determined. The microbial structure of the biomass from the digesters was investigated with use of next-generation sequencing.The percentage of methanogens in the biomass reached 21%, resulting in high quality biogas (over 61% methane content). The abundance of syntrophic bacteria was 4.47%, and stable methane production occurred at a Methanomicrobia to Synergistia ratio of 4.6:1.0. The two most numerous genera of methanogens (about 11% total) wereMethanosaetaandMethanolinea, indicating that, at the low substrate loading in the digester, the acetoclastic and hydrogenotrophic paths of methane production were equally important. The high abundance of the orderBacteroidetes, including the classCytophagia(11.6% of all sequences), indicated the high potential of the biomass for efficient degradation of lignocellulitic substances, and for degradation of protein and amino acids to acetate and ammonia.This study sheds light on the ecology of microbial groups that are involved in mesophilic fermentation in mature, stably-performing microbiota in full-scale reactors fed with sewage sludge under low substrate loading.


Author(s):  
Reza Barati Rashvanlou ◽  
Mahdi Farzadkia ◽  
Abbas Ali Moserzadeh ◽  
Asghar Riazati ◽  
Chiang Wei ◽  
...  

Introduction: One of biological wastewater treatment methods that utilizes to both digesting waste activated sludge and methane production is anaerobic digestion (AD). It is believed to be most effective solution in terms of energy crisis and environmental pollution issues. Materials and Methods: In this study the sludge was digested anaerobically sampled from a full-scale WWTP, located at south of Tehran, Iran for evaluation. To study the microbial community within the sludge the MiSeq Sequencing method utilized. Based on our field data (data not shown) and microbial community data, a schematic diagram of probable leading pathways was made in the studied digester. Results: At first, the community variety in the bulk sludge and richness were enhanced followed by loading increasing. Meanwhile, the loading change enhanced the community richness and variety of the sludge. By comparing the rank-abundance distributions, a shallow gradient would show high evenness since the abundances of diverse species are alike. The results showed all the communities were extremely diverse and 15 phyla were distinguished in the sludge sample. The dominant phyla of the community were Bacteroidetes and Firmicutes and quantity of the two phyla were 21% and 11%, respectively. Anaerobaculum, Acinetobacter, Syntrophomonas, and Coprothermobacter were the chief genera for the microbial communities and the sum of four genera were 7%, 3%, 3%, and 2%, respectively. Conclusion: It was shown that syntrophic acetate oxidizing bacterias (SAOBs) metabolized acetate through hydrogen trophic methanogenesis in the digester. Generally, the findings may be useful to help the wastewater operators to utilize an effective method that able to treat waste sludge plus methane production, simultaneously.


2018 ◽  
Vol 129 ◽  
pp. 58-65 ◽  
Author(s):  
Jing Sun ◽  
Bing-Jie Ni ◽  
Keshab Raj Sharma ◽  
Qilin Wang ◽  
Shihu Hu ◽  
...  

2020 ◽  
Vol 14 (4) ◽  
pp. 551-557
Author(s):  
Yongku Li ◽  
Xiaomin Hu ◽  
Lei Feng

The changing parameters, as the biogas production rate, the methane production rate, the cumulative biogas amount, the cumulative methane amount, the biogas composition, pH etc. in high temperature anaerobic fermentation of chicken manure and stalks were analyzed by experiments with different mass ratios of chicken manure or livestock manure and stalks with a high C/N ratio. The methane production mechanism of high temperature anaerobic digestion of chicken manure and stalks was discussed in detail. It showed that not only the biogas production rates but also the methane production rates of R1–R7 demonstrated the trend of initial increase and then decrease after 50 d of high temperature anaerobic digestion. Besides, the gas production of R1 with pure chicken manure stopped on the 30th d of the reaction. The gas production of other groups R2–R7 also stopped on the corresponding 34th, 36th, 36th, 37th, 37th, and 37th day, respectively. At the end of the reaction, the cumulative biogas amounts and the cumulative methane amounts of R1–R7 were 411.58 and 269.54, 459.91 and 314.41, 425.32 and 294.11, 401.85 and 272.54, 382.63 and 257.07, 363.04 and 218.16, and 257.15 and 160.10 N ml/(g VS). The biogas slurry pH of R1–R7 all demonstrated a trend of initial decrease and then increase, e. g., pH of R2 reached the minimum of 5.94 on the 5th day. pH values of other groups were between 6.01 and 6.39. After the addition of 4 g of sodium bicarbonate on the 7th day, biogas slurry pH of R1–R7 all increased. pH was maintained between 7.16 and 7.44 until the end of the reaction.


2006 ◽  
Vol 53 (8) ◽  
pp. 23-32 ◽  
Author(s):  
D. Bolzonella ◽  
P. Pavan ◽  
S. Mace ◽  
F. Cecchi

This paper presents a comparison of dry anaerobic digestion reactors fed with differently sorted municipal organic solid wastes. One reactor was fed with source sorted organic wastes and a second reactor was fed with mixed organic wastes consisting of grey wastes, mechanically selected municipal solid wastes and sludge. The two reactors utilised the same process (Valorga) and operational conditions at full scale. The results of the study emphasise the influence of the kind of treated material on the process performances, especially in terms of biogas and methane production, thus, energy reclamation. The reactor treating the source sorted organic waste and the reactor treating the mixed organic wastes generated some 200 m3 and 60 m3 of biogas per ton of waste treated, respectively, while the specific methane production was some 0.40 and 0.13 m3CH4/kgTVS, respectively. The mass balance and the final fate of the digested material from the two reactors were also clearly different. As for the costs, these were some 29 € per ton of treated waste (50% for personnel) and 53 €/ton for disposing of the rejected materials. Incomes were some 100 €/ton (on average) and an other 15 €/ton came from green certificates. The initial investment was 16 million Euros.


2009 ◽  
Vol 43 (5) ◽  
pp. 315-321 ◽  
Author(s):  
HIDEYOSHI YOSHIOKA ◽  
SUSUMU SAKATA ◽  
BARRY A. CRAGG ◽  
R. JOHN PARKES ◽  
TETSUYA FUJII

2021 ◽  
Vol 1 (3) ◽  
pp. 1-8
Author(s):  
Ravikiran Shet ◽  
Srikanth Mutnuri

India generates 0.15 million metric tons (MT) of solid waste per day out of which more than 80% is organic fraction. Apart from this, 38% of the households use septic tanks where proper disposal of faecal sludge is also need of the hour. Anaerobic co-digestion (ACD) of two different substrates has positive potential towards solving this problem. In the present study, ACD of organic fraction of municipal solid waste (OFMSW) and septage solids (SS) was studied at three different levels, i.e., lab-scale, pilot-scale (1 m3), and full scale- capacity (325 m3). A loading rate of 1.5 kg VS/m3 was selected. The bio-methanation potential (BMP) assay showed a maximum biogas generation, i.e., 120±20.6 mL/gmVS with 68% maximum methane concentration at a 5:1 OFMSW and SS ratio. Cumulative biogas production after 30 days was 1.6 L/gmVS. The ultimate biogas production in the pilot-scale plant was 1000±100.5 L/day with 71% methane. The plant was also efficient in removing 87% of COD and 61% of VS. The full-scale anaerobic digester was set up at Mormugao Municpal Council, Goa India wherein the objective was to co-digest OFMSW and SS. This digester showed a similar removal pattern like earlier studies i.e., 94% and 45% COD and VS removal, respectively. The average methane content of the biogas was 68%. Full-scale operation of the anaerobic digester did not show any operational problems at the chosen co-digestion conditions.


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