scholarly journals Effect of a sublethal high-pressure homogenization treatment on the fatty acid membrane composition of probiotic lactobacilli

2013 ◽  
Vol 58 (2) ◽  
pp. 109-117 ◽  
Author(s):  
G. Tabanelli ◽  
F. Patrignani ◽  
F. Gardini ◽  
G. Vinderola ◽  
J. Reinheimer ◽  
...  
2012 ◽  
Vol 32 (2) ◽  
pp. 302-307 ◽  
Author(s):  
Giulia Tabanelli ◽  
Patricia Burns ◽  
Francesca Patrignani ◽  
Fausto Gardini ◽  
Rosalba Lanciotti ◽  
...  

2020 ◽  
Vol 8 (3) ◽  
pp. 439 ◽  
Author(s):  
Lorenzo Siroli ◽  
Giacomo Braschi ◽  
Samantha Rossi ◽  
Davide Gottardi ◽  
Francesca Patrignani ◽  
...  

Sub-lethal high-pressure homogenization treatments applied to Lactobacillus paracasei A13 demonstrated to be a useful strategy to enhance technological and functional properties without detrimental effects on the viability of this strain. Modification of membrane fatty acid composition is reported to be the main regulatory mechanisms adopted by probiotic lactobacilli to counteract high-pressure stress. This work is aimed to clarify and understand the relationship between the modification of membrane fatty acid composition and the expression of genes involved in fatty acid biosynthesis in Lactobacillus paracasei A13, before and after the application of different sub-lethal hyperbaric treatments. Our results showed that Lactobacillus paracasei A13 activated a series of reactions aimed to control and stabilize membrane fluidity in response to high-pressure homogenization treatments. In fact, the production of cyclic fatty acids was counterbalanced by the unsaturation and elongation of fatty acids. The gene expression data indicate an up-regulation of the genes accA, accC, fabD, fabH and fabZ after high-pressure homogenization treatment at 150 and 200 MPa, and of fabK and fabZ after a treatment at 200 MPa suggesting this regulation of the genes involved in fatty acids biosynthesis as an immediate response mechanism adopted by Lactobacillus paracasei A13 to high-pressure homogenization treatments to balance the membrane fluidity. Although further studies should be performed to clarify the modulation of phospholipids and glycoproteins biosynthesis since they play a crucial role in the functional properties of the probiotic strains, this study represents an important step towards understanding the response mechanisms of Lactobacillus paracasei A13 to sub-lethal high-pressure homogenization treatments.


2014 ◽  
Vol 62 (39) ◽  
pp. 9539-9543 ◽  
Author(s):  
Natalia Toro-Funes ◽  
Joan Bosch-Fusté ◽  
M. Teresa Veciana-Nogués ◽  
M. Carmen Vidal-Carou

2008 ◽  
Vol 56 (16) ◽  
pp. 7125-7130 ◽  
Author(s):  
Julieta Pereda ◽  
Victoria Ferragut ◽  
Joan Miquel Quevedo ◽  
Buenaventura Guamis ◽  
Antonio J. Trujillo

2013 ◽  
Vol 160 (3) ◽  
pp. 273-281 ◽  
Author(s):  
Francesca Patrignani ◽  
Giulia Tabanelli ◽  
Lorenzo Siroli ◽  
Fausto Gardini ◽  
Rosalba Lanciotti

2020 ◽  
Vol 16 (3) ◽  
Author(s):  
Henan Zou ◽  
Ning Zhao ◽  
Xiaojie Shi ◽  
Shuang Sun ◽  
Cuiping Yu

AbstractThis study investigated the changes in physicochemical and functional properties of water-soluble protein from mussels (MWP) induced by high-pressure homogenization (HPH). The results indicated that HPH treatment unfolded or disrupted the initial structure of MWP, exposing free sulfhydryl groups and buried hydrophobic groups. As the homogenization pressure increased, the aggregation of MWP particles gradually decreased. Moreover, protein solubility and dispersion stability increased in aqueous solution. Foaming and emulsifying properties were also improved. HPH treatment has proven to be an effective technique for enhancing the functional properties of shellfish protein, and 120 MPa was the optimum homogenization pressure to modify MWP.


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