scholarly journals A large potentiation effect of serum on the in vitro potency of tulathromycin against Mannheimia haemolytica and Pasteurella multocida

2016 ◽  
Vol 40 (5) ◽  
pp. 419-428 ◽  
Author(s):  
P. Lees ◽  
J. Illambas ◽  
T. J. Potter ◽  
L. Pelligand ◽  
A. Rycroft ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Mannheimia Haemolytica ◽  
Potentiation Effect

scholarly journals Susceptibilidade in vitro a antimicrobianos da Mannheimia haemolytica e da Pasteurella multocida isoladas de ovinos sadios e com doenças respiratórias

2007 ◽  
Vol 44 (supl.) ◽  
pp. 111
Author(s):  
Leomar Viana ◽  
Roberto Calderon Gonçalves ◽  
José Paes de Oliveira Filho ◽  
Antônio Carlos Paes ◽  
Simone Biagio Chiacchio ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Mannheimia Haemolytica

Pasteurella multocida e Mannheimia haemolytica (P. haemolytica) estão associadas a enfermidades no sistema respiratório de ovinos. Com o objetivo de avaliar a susceptibilidade in vitro destes microrganismos frente aos antimicrobianos, foram colhidas amostras de nasofaringe (n=180) e orofaringe (n=82) de ovinos com e sem enfermidade respiratória. Dentre os antimicrobianos testados, a sensibilidade foi maior para enrofloxacina (100%) e florfenicol (100%), considerando-se ambas as espécies bacterianas. Observou-se resistência de M. haemolytica e P. multocida à tetraciclina (15,64% e 17,65%, respectivamente) e penicilina (1.82% e 4.2%, respectivamente).

scholarly journals A ten-year (2000–2009) study of antimicrobial susceptibility of bacteria that cause bovine respiratory disease complex—Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni—in the United States and Canada

2012 ◽  
Vol 24 (5) ◽  
pp. 932-944 ◽  
Author(s):  
Ellen Portis ◽  
Cynthia Lindeman ◽  
Lacie Johansen ◽  
Gillian Stoltman
Keyword(s):  
Pasteurella Multocida ◽  
Inhibitory Concentration ◽  
The United States ◽  
Mannheimia Haemolytica ◽  
In Vitro Susceptibility ◽  
Histophilus Somni ◽  
Bovine Respiratory Disease Complex ◽  
Year 2000 ◽  
Over Time

Bovine isolates of Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni, collected from 2000 to 2009, were tested for in vitro susceptibility to ceftiofur, penicillin, danofloxacin, enrofloxacin, florfenicol, tetracycline, tilmicosin, and tulathromycin. Ceftiofur remained very active against all isolates. Penicillin retained good activity against P. multocida and H. somni isolates with no appreciable changes in susceptibility or minimal inhibitory concentration (MIC) distributions with time. While there was no obvious trend, the percent of M. haemolytica that were susceptible to penicillin ranged from 40.9% to 66.7%. Danofloxacin MIC50 and MIC90 values for M. haemolytica and P. multocida did not change beyond a single dilution over the 6 years it was included in the testing panel. The MIC90 for H. somni increased beyond 1 dilution. Enrofloxacin MIC50 values for the 3 pathogens also did not change over time, unlike the MIC90 values, which increased by at least 4-doubling dilutions. Ninety percent or more of M. haemolytica and H. somni isolates were susceptible to florfenicol, while susceptibility among P. multocida was 79% or greater. Less than 50% of the isolates tested as susceptible to tetracycline in many of the years. All 3 organisms showed declines in tilmicosin and tulathromycin MIC50 and MIC90 values over the years in which they were tested.

scholarly journals Prevalence and antibiotic susceptibility of Mannheimia haemolytica and Pasteurella multocida isolated from ovine respiratory infection: A study from Karnataka, Southern India

2020 ◽  
Vol 13 (9) ◽  
pp. 1947-1954
Author(s):  
Swati Sahay ◽  
Krithiga Natesan ◽  
Awadhesh Prajapati ◽  
Triveni Kalleshmurthy ◽  
Bibek Ranjan Shome ◽  
...  
Keyword(s):  
Respiratory Infection ◽  
Antibiotic Susceptibility ◽  
Pasteurella Multocida ◽  
Susceptibility Testing ◽  
Infected Sheep ◽  
Mannheimia Haemolytica ◽  
Economic Losses ◽  
Antibiotic Susceptibility Testing ◽  
Sheep Population

Background and Aim: Respiratory infection due to Mannheimia haemolytica and Pasteurella multocida are responsible for huge economic losses in livestock sector globally and it is poorly understood in ovine population. The study aimed to investigate and characterize M. haemolytica and P. multocida from infected and healthy sheep to rule out the involvement of these bacteria in the disease. Materials and Methods: A total of 374 healthy and infected sheep samples were processed for isolation, direct detection by multiplex PCR (mPCR), and antibiotic susceptibility testing by phenotypic and genotypic methods. Results: Overall, 55 Pasteurella isolates (27 [7.2%] M. haemolytica and 28 [7.4%] P. multocida) were recovered and identified by bacteriological tests and species-specific PCR assays. Significant correlation between the detection of M. haemolytica (66.6%) with disease condition and P. multocida (19.1%) exclusively from infected sheep was recorded by mPCR. In vitro antibiotic susceptibility testing of 55 isolates revealed higher multidrug resistance in M. haemolytica (25.9%) than P. multocida (7.1%) isolates. Descending resistance towards penicillin (63.6%), oxytetracycline (23.6%), streptomycin (14.5%), and gentamicin (12.7%) and absolute sensitivity towards chloramphenicol were observed in both the pathogens. The antibiotic resistance genes such as strA (32.7%) and sul2 (32.7%) associated with streptomycin and sulfonamide resistance, respectively, were detected in the isolates. Conclusion: The study revealed the significant involvement of M. haemolytica together with P. multocida in ovine respiratory infection and is probably responsible for frequent disease outbreaks even after vaccination against hemorrhagic septicemia in sheep population of Karnataka, southern province of India.

scholarly journals Perfil de sensibilidade de amostras de Pasteurella multocida e Mannheimia haemolytica isoladas na região de Botucatu, São Paulo, Brasil, no período de janeiro de 2000 a novembro de 2007

2009 ◽  
Vol 16 (1) ◽  
pp. 173-179 ◽  
Author(s):  
Taíssa Cook Siqueira Soares ◽  
Rodrigo Garcia Motta ◽  
Antônio Carlos `Paes ◽  
Fernando José Paganini Listoni
Keyword(s):  
Pasteurella Multocida ◽  
Sao Paulo ◽  
Mannheimia Haemolytica ◽  
São Paulo

A terapia antimicrobiana ainda é a ferramenta de escolha para o controle de infecções causadas pelos gêneros Pasteurella e Mannheimia. O trabalho apresenta os resultados dos Testes de Sensibilidade a Antimicrobianos, realizados através do método de difusão pelo sistema de discos, de 71 amostras de Pasteurella multocida e 71 de Mannheimia haemolytica isoladas de várias espécies animais, provenientes da região de Botucatu, São Paulo, Brasil, entre Janeiro de 2000 e Novembro de 2007. Os resultados demonstram grande diferença de sensibilidade entre os isolados, variando de 74,54% (sulfa- trimetoprim) a 100,00% (ciprofloxacina e florfenicol), em relação à P. multocida e de 80,05% (gentamicina) a 100,00% (ampicilina), em relação à M. haemolytica, além da ocorrência de resistência múltipla. Estes dados indicam a ocorrência de seleção de cepas resistentes, devido ao uso indiscriminado de antimicrobianos, e a importância de testar a sensibilidade, in vitro, de cepas patogênicas envolvidas em casos clínicos, para definir a terapia antimicrobiana mais apropriada.

Molecular Detection of Bacterial Pathogens Directly from the Nasal Swabs and Lung Tissue of Sheep and Goats

2019 ◽  
Vol 15 (02) ◽  
pp. 22-25
Author(s):  
Sunaina Thakur ◽  
Subhash Verma ◽  
Prasenjit Dhar ◽  
Mandeep Sharma
Keyword(s):  
Pasteurella Multocida ◽  
Direct Detection ◽  
Bacterial Species ◽  
Economic Losses ◽  
Isolation And Identification ◽  
Sheep And Goats ◽  
Histophilus Somni ◽  
Nasal Swabs ◽  
Mycoplasma Spp

Respiratory infections of sheep and goats cause heavy morbidity and mortality, leading to huge economic losses. Conventional methods of diagnosis that include isolation and identification of incriminating microbes are time-consuming and fraught with logistic challenges. Direct detection of incriminating microbes using molecular tools is gaining popularity in clinical, microbiological settings. In this study, a total of 50 samples (44 nasal swabs and 6 lung tissues) from sheep and goats were screened for the detection of different bacterial species by in vitro amplification of genus or species-specific genes. Histophilus somni was detected in 2% goat samples, Trueperella pyogenes in 20% goat nasal swabs, whereas 22% goat nasal swab samples were found positive for Mycoplasma spp. None of the samples from sheep was detected positive for H. somni, T. pyogenes, Mycoplasma spp. Similarly, all samples, irrespective, whether from sheep or goats, showed negative results for Pasteurella multocida, Mannheimia haemolytica, and Corynebacterium pseudotuberculosis.

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