scholarly journals Detection of pathogens in blood or feces of adult horses with enteric disease and association with outcome of colitis

2021 ◽  
Author(s):  
Jamie J. Kopper ◽  
Jaclyn A. Willette ◽  
Clark J. Kogan ◽  
Alexis Seguin ◽  
Steven R. Bolin ◽  
...  
Keyword(s):  
Enteric Disease

Scanning Microscopy of Human Intestinal Explants in Organ Culture

1972 ◽  
Vol 30 ◽  
pp. 396-397
Author(s):  
Bruce Wetzel ◽  
Robert Buscho ◽  
Raphael Dolin
Keyword(s):  
Electron Microscopy ◽  
Room Temperature ◽  
Culture Conditions ◽  
Human Fetuses ◽  
Enteric Disease ◽  
Organ Cultures ◽  
Growth Of A Variety ◽  
Normal Human ◽  
Fetal Intestine ◽  
Scanning Electron

It has been reported that explants of human fetal intestine can be maintained in culture for up to 21 days in a viable condition and that these organ cultures support the growth of a variety of known viral agents responsible for enteric disease. Scanning electron microscopy (SEM) has been undertaken on several series of these explants to determine their appearance under routine culture conditions.Fresh specimens of jejunum obtained from normal human fetuses were washed, dissected into l-4mm pieces, and cultured in modified Leibowitz L-15 medium at 34° C as previously described. Serial specimens were fixed each day in 3% glutaraldehyde for 90 minutes at room temperature, rinsed, dehydrated, and dried by the CO2 critical point method in a Denton DCP-1 device. Specimens were attached to aluminum stubs with 3M transfer tape No. 465, and one sample on each stub was carefully rolled along the adhesive such that villi were broken off to expose their interiors.

scholarly journals Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 656
Author(s):  
Konstantin Tanida ◽  
Andreas Hahn ◽  
Kirsten Alexandra Eberhardt ◽  
Egbert Tannich ◽  
Olfert Landt ◽  
...  
Keyword(s):  
Real Time ◽  
Indigenous People ◽  
Real Time Pcr ◽  
Latent Class ◽  
Enterocytozoon Bieneusi ◽  
Enteric Disease ◽  
Immunosuppressed Patients ◽  
Study Population ◽  
Stool Samples ◽  
Pcr Assays

Microsporidiosis is an infection predominantly occurring in immunosuppressed patients and infrequently also in travelers. This study was performed to comparatively evaluate the diagnostic accuracy of real-time PCR assays targeting microsporidia with etiological relevance in the stool of human patients in a latent class analysis-based test comparison without a reference standard with perfect accuracy. Thereby, two one-tube real-time PCR assays and two two-tube real-time PCR assays targeting Enterocytozoon bieneusi and Encephalocytozoon spp. were included in the assessment with reference stool material (20), stool samples from Ghanaian HIV-positive patients (903), and from travelers, migrants and Colombian indigenous people (416). Sensitivity of the assays ranged from 60.4% to 97.4% and specificity from 99.1% to 100% with substantial agreement according to Cohen’s kappa of 79.6%. Microsporidia DNA was detected in the reference material and the stool of the HIV patients but not in the stool of the travelers, migrants, and the Colombian indigenous people. Accuracy-adjusted prevalence was 5.8% (n = 78) for the study population as a whole. In conclusion, reliable detection of enteric disease-associated microsporidia in stool samples by real-time PCR could be demonstrated, but sensitivity between the compared microsporidia-specific real-time PCR assays varied.

scholarly journals Timeliness of Enteric Disease Surveillance in 6 US States

2008 ◽  
Vol 14 (2) ◽  
pp. 311-313 ◽  
Author(s):  
Craig W. Hedberg ◽  
Jesse F. Greenblatt ◽  
Bela T. Matyas ◽  
Jennifer Lemmings ◽  
Donald J. Sharp ◽  
...  
Keyword(s):  
Disease Surveillance ◽  
Enteric Disease ◽  
Us States

Enteric Disease and Malnutrition

1985 ◽  
Vol 4 (1) ◽  
pp. 155-156
Author(s):  
Carmen Gutiérrez ◽  
Ernesto Guiraldes
Keyword(s):  
Enteric Disease

scholarly journals Diagnosing clostridial enteric disease in poultry

2013 ◽  
Vol 25 (3) ◽  
pp. 314-327 ◽  
Author(s):  
Kerry K. Cooper ◽  
J. Glenn Songer ◽  
Francisco A. Uzal
Keyword(s):  
Enteric Disease

scholarly journals Assessment of fomites and infection risk to seniors in recreational curling

2020 ◽  
Author(s):  
Elliot Spicer ◽  
BCIT School of Health Sciences, Environmental Health ◽  
Helen Heacock
Keyword(s):  
Disease Transmission ◽  
Sampling Error ◽  
Total Coliform ◽  
Infection Risk ◽  
Age Group ◽  
Enteric Disease ◽  
Health Promotion And Education ◽  
Increased Risk ◽  
Colony Forming Units ◽  
Risk Of Disease

Background: Seniors participate in sports to improve physical, mental, and social health; however, such activities may increase the risk of illness and injury. Curling is popular in this age group because it is physically manageable, strategic, and provides social connection. Certain factors in curling such as handshaking, play during the flu season, and shared contact with curling stones suggest an increased risk of disease transmission. The purpose of this study was to determine the qualitative risk of communicable enteric disease transmission due to shared contact with curling stone handles in a senior men’s curling league. Methods: 3M™ Quick Swabs were used to sample 22 curling stone handles for total coliforms before a senior’s league game. To analyze microbial shedding during gameplay, the same 22 handles were sampled after the game. Samples were plated on 3M™ Petrifilm™ Coliform Count Plates and incubated at 30ºC ± 1ºC for 24 hours ± 2 hours. Colonies were enumerated in units of CFU (colony forming units)/cm2. Ambient and handle surface temperatures were measured, and curler hygiene-related behaviours documented. Results: Total coliform counts for all samples were 0 CFU/cm2. The ambient temperature was 6.6°C pre-game, and 8.0°C post-game. Mean handle surface temperature was 3.6°C. Hygiene behaviours of concern were hand-face contact, handkerchief/tissue use, and handshaking. Conclusion: There is low risk of enteric disease transmission due to shared contact with curling stone handles by male curlers 55 years and older. Absence of coliforms may have been due to adequate player hygiene, transference of microbial load before sampling, error, or environmental conditions. Health promotion and education can reduce the infection risk elevated by poor hand hygiene, face contact, and handshaking in senior’s curling, thereby protecting the health and welfare of all participants.

scholarly journals Overview of the Impact of Epidemic-Assistance Investigations of Foodborne and Other Enteric Disease Outbreaks, 1946-2005

2011 ◽  
Vol 174 (suppl 11) ◽  
pp. S23-S35 ◽  
Author(s):  
A. P. Wright ◽  
L. H. Gould ◽  
B. Mahon ◽  
M. J. Sotir ◽  
R. V. Tauxe
Keyword(s):  
Disease Outbreaks ◽  
Enteric Disease ◽  
The Impact

scholarly journals BUTTERMILK IN ENTERIC DISEASE.

The Lancet ◽  
1907 ◽  
Vol 170 (4385) ◽  
pp. 794
Author(s):  
FrancisT. Bond
Keyword(s):  
Enteric Disease
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