scholarly journals Assessment of symmetric dimethylarginine as a biomarker of renal function in hyperthyroid cats treated with radioiodine

2019 ◽  
Vol 33 (2) ◽  
pp. 516-522 ◽  
Author(s):  
Eva Buresova ◽  
Emmelie Stock ◽  
Dominique Paepe ◽  
Lisa Stammeleer ◽  
Eva Vandermeulen ◽  
...  
Keyword(s):  
Renal Function ◽  
Symmetric Dimethylarginine

scholarly journals Evaluation of renal biomarkers, including symmetric dimethylarginine, following gentamicin-induced proximal tubular injury in the rat

Kidney360 ◽  
2021 ◽  
pp. 10.34067/KID.0006542020
Author(s):  
Diane M Hamlin ◽  
A. Eric Schultze ◽  
Michael J. Coyne ◽  
Donald J. McCrann ◽  
Rebekah Mack ◽  
...  
Keyword(s):  
Renal Function ◽  
Control Group ◽  
Analytical Sensitivity ◽  
Symmetric Dimethylarginine ◽  
Serum Cystatin C ◽  
Rat Serum ◽  
Proximal Tubular ◽  
Renal Biomarker ◽  
Biomarker Evaluation ◽  
Dose Levels

Symmetric dimethylarginine (SDMA) is an excretory renal function biomarker shown to correlate well with glomerular filtration rate in dogs, cats, humans, and rats. The objectives of this study were to determine utility of serum SDMA as a renal biomarker in a rat model of gentamicin-induced renal injury and provide validation of a commercially available SDMA immunoassay for rat serum. Rats were randomly assigned to one of three dose levels of gentamicin (20, 50, or 100 mg/kg) or a vehicle control group and dosed once daily by subcutaneous injection for either four or ten days. Serum and urine renal biomarker evaluation including serum SDMA, hematologic and serum biochemical analysis, urinalysis, and histologic examination of kidney were performed. Prior to biologic validation, analytic validation of the SDMA immunoassay for rat serum was performed including assessment of assay accuracy, precision, analytical sensitivity, linearity, analyte stability, and interference testing. Among markers of excretory renal function SDMA and serum creatinine increased earliest and at the lowest gentamicin concentrations and were significantly increased in both the 50 mg/kg and the 100 mg/kg dosage levels in the 4 and 10-dose treatment groups compared to controls. Time- and dose-dependent increases were noted for all urinary biomarkers investigated in this study, with µALB being most responsive and OPN least responsive for detection of gentamicin-induced injury across dose levels and schedules investigated. The SDMA immunoassay met all set quality requirements assessed in analytical validation. This study is the first to investigate performance of serum SDMA as compared to other excretory renal function markers in a rat gentamicin acute toxicity model. In this study serum SDMA was an earlier biomarker for detection of gentamicin-induced toxicity than serum cystatin C, BUN, and creatinine clearance. The SDMA immunoassay provides a reliable commercially available assay for future renal investigations in rat models.

scholarly journals Symmetric dimethylarginine and renal function analysis in horses with dehydration

2021 ◽  
Author(s):  
Hsiao‐Chien Lo ◽  
Judith C. Winter ◽  
Heidrun Gehlen ◽  
Roswitha Merle
Keyword(s):  
Renal Function ◽  
Function Analysis ◽  
Symmetric Dimethylarginine

Symmetric Dimethylarginine Is a Sensitive Biomarker of Glomerular Injury in Rats

2021 ◽  
pp. 019262332110453
Author(s):  
Rebecca Kohnken ◽  
Lauren Himmel ◽  
Michael Logan ◽  
Richard Peterson ◽  
Sabyasachi Biswas ◽  
...  
Keyword(s):  
Renal Function ◽  
Renal Injury ◽  
Periodic Acid ◽  
Kidney Tissue ◽  
Glomerular Injury ◽  
Symmetric Dimethylarginine ◽  
Puromycin Aminonucleoside ◽  
Periodic Acid Schiff ◽  
Gold Standard Method ◽  
Toxicity Studies

Glomerular filtration rate is the gold-standard method for assessment of renal function but is rarely performed in routine toxicity studies. Standard serum biomarkers of renal function are insensitive and become elevated only with significant loss of organ function. Symmetric dimethylarginine (SDMA) is a ubiquitous analyte that is freely filtered by the glomerulus and can be detected in serum. It has shown utility for the detection of renal injury in dogs and cats in clinical veterinary practice, but the potential utility of SDMA to detect renal injury in preclinical species or toxicity studies has not been thoroughly investigated. We utilized a well-characterized glomerular toxicant, puromycin aminonucleoside, to induce podocyte injury and subsequent proteinuria in young male Sprague-Dawley rats. At the end of 1 or 2 weeks, blood, urine, and kidney tissue were collected for analysis. One week following a single 50 mg/kg dose, urea nitrogen, creatinine, and albumin mean values were within historical control ranges, while SDMA was increased. Glomerular changes in these animals included periodic acid–Schiff positive globules within podocytes, podocyte hypertrophy by light microscopy, and podocyte degeneration with effacement of foot processes by electron microscopy (EM). Taken together, our data indicate that SDMA may be a useful biomarker for early detection of glomerular toxicities in rats.

Symmetric dimethylarginine is a marker of detrimental outcome in the acute phase after ischaemic stroke: role of renal function

2011 ◽  
Vol 122 (3) ◽  
pp. 105-111 ◽  
Author(s):  
Nicole Lüneburg ◽  
Rouven-Alexander von Holten ◽  
Rudolf F. Töpper ◽  
Edzard Schwedhelm ◽  
Renke Maas ◽  
...  
Keyword(s):  
Renal Function ◽  
Clinical Outcome ◽  
Ischaemic Stroke ◽  
Acute Ischaemic Stroke ◽  
Plasma Levels ◽  
Recurrent Stroke ◽  
Composite Endpoint ◽  
Adverse Clinical Outcome ◽  
Symmetric Dimethylarginine ◽  
Stroke Event

Methylarginines have been shown to interfere with NO (nitric oxide) formation by inhibiting NOS (NO synthase)–ADMA (asymmetric dimethylarginine) and cellular L-arginine uptake into the cell [ADMA and SDMA (symmetric dimethylarginine)]. In a recent study, elevation of SDMA was related to long-term mortality in patients recruited 30 days after a stroke event. In the present study, we aimed at investigating the association of SDMA and adverse clinical outcome in the early phase (first 30 days) after acute ischaemic stroke. A total of 137 patients were recruited immediately upon admission to the emergency unit with an acute ischaemic stroke. Plasma levels of methylarginines were determined by a validated LC–MS/MS (liquid chromatography–tandem MS) method. Patients were prospectively followed for 30 days. A total of 25 patients (18.2%) experienced the primary composite endpoint [death, recurrent stroke, MI (myocardial infarction) and rehospitalization]. SDMA plasma levels were significantly higher in stroke patients compared with patients without event (0.89±0.80 compared with 0.51±0.24 μmol/l; P<0.001). SDMA levels were significantly correlated with markers of renal function. Kaplan–Meier survival analysis demonstrated that cumulative survival decreased significantly with ascending tertiles of SDMA (P<0.001). Our study provides the first data indicating that SDMA is strongly associated with adverse clinical outcome during the first 30 days after ischaemic stroke. Our results strengthen the prognostic value of renal function in patients with stroke and confirm the hypothesis that SDMA is a promising marker for renal function.

Is plasma symmetric dimethylarginine a suitable marker of renal function in children and adolescents?

2011 ◽  
Vol 46 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Anna Wasilewska ◽  
Katarzyna Taranta-Janusz ◽  
Walentyna Zoch-Zwierz ◽  
Joanna Michaluk-Skutnik
Keyword(s):  
Renal Function ◽  
Children And Adolescents ◽  
Symmetric Dimethylarginine ◽  
Suitable Marker

scholarly journals Bariatric Surgery Improves the Atherogenic Profile of Circulating Methylarginines in Obese Patients: Results from a Pilot Study

Metabolites ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 759
Author(s):  
Julie Poirier ◽  
Chloé Cloteau ◽  
Audrey Aguesse ◽  
Xavier Billot ◽  
Etienne Thévenot ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Bariatric Surgery ◽  
Renal Function ◽  
Asymmetric Dimethylarginine ◽  
Liver Steatosis ◽  
The Body ◽  
Obese Patients ◽  
Healthy Individuals ◽  
Symmetric Dimethylarginine ◽  
Cardiometabolic Disorders

Bariatric surgery improves obesity-related comorbidities. Methylarginines are biomarkers of cardiometabolic risk, liver steatosis, and insulin resistance. Here, we aimed to investigate methylarginines in obese patients undergoing bariatric surgery and compared them to age- and sex-matched healthy subjects. Thirty-one obese patients who underwent bariatric surgery and 31 healthy individuals were used for this retrospective study. The basal serum methylarginine levels were determined in the healthy individuals and the obese patients, before surgery and 6 and 12 months after surgery, by mass spectrometry. Compared with the healthy individuals, the obese patients displayed elevated monomethylarginine (mean change: +95%, p < 0.001), asymmetric-dimethylarginine (+105%, p < 0.001), symmetric-dimethylarginine (+25%, p = 0.003), and dimethylguanidino valerate (+32%, p = 0.008) concentrations. Bariatric surgery durably reduced the body mass index by 28% (12 months, 95%CI: 24–33, p = 0.002) and improved plasma lipids, insulin resistance, and liver function. Bariatric surgery reduced the serum levels of monomethylarginine and asymmetric-dimethylarginine by 12% (95%CI: 6–17) and 36% (95%CI: 27–45) (12 months, p = 0.003), respectively, but not symmetric-dimethylarginine or dimethylguanidino valerate. The monomethylarginine and asymmetric-dimethylarginine concentrations were strongly correlated with markers of dyslipidemia, insulin resistance, and a fatty liver. Serum dimethylguanidino valerate was primarily correlated with glycemia and renal function, whereas serum symmetric-dimethylarginine was almost exclusively associated with renal function. In conclusion, the monomethylarginine and asymmetric-dimethylarginine levels are efficiently decreased by bariatric surgery, leading to a reduced atherogenic profile in obese patients. Methylarginines follow different metabolic patterns, which could help for the stratification of cardiometabolic disorders in obese patients.

scholarly journals Symmetric dimethylarginine (SDMA) as endogenous marker of renal function—a meta-analysis

2006 ◽  
Vol 21 (9) ◽  
pp. 2446-2451 ◽  
Author(s):  
Jan T. Kielstein ◽  
Shelley R. Salpeter ◽  
Stefanie M. Bode-Boeger ◽  
John P. Cooke ◽  
Danilo Fliser
Keyword(s):  
Renal Function ◽  
Meta Analysis ◽  
Symmetric Dimethylarginine

Assessment of serum symmetric dimethylarginine and creatinine concentrations in cats with urethral obstruction

2021 ◽  
pp. 1098612X2110606
Author(s):  
Kendall E Wilson ◽  
Allyson C Berent ◽  
Chick W Weisse ◽  
Donald Szlosek
Keyword(s):  
Renal Function ◽  
Prospective Observational Study ◽  
Urine Culture ◽  
Initial Presentation ◽  
Urethral Obstruction ◽  
Symmetric Dimethylarginine ◽  
Urethral Catheterization ◽  
Creatinine Concentration ◽  
The Mean

Objectives The aims of this study were to evaluate serum symmetric dimethylarginine (SDMA) and creatinine concentrations in cats with urethral obstruction pre- and post-decompression of the obstruction, and to determine if pre-decompression values were predictive of post-decompression renal function, as measured by SDMA and creatinine. Methods This was a prospective observational study. Twenty-five client-owned cats with urethral obstruction were hospitalized for decompression of the obstruction. Serum SDMA and creatinine were prospectively assessed at presentation, 24 h post-decompression and 5–20 days post-decompression. Urinalysis and culture were assessed at presentation and at the final follow-up. Exclusion criteria included positive urine culture, reobstruction or failure to obtain required samples. Results Mean SDMA concentration dropped by 41.8% from an initial pre-decompression concentration of 17.6 µg/dl to 10.3 µg/dl 24 h post-decompression ( P <0.001). The mean creatinine value dropped by 38.4% from an initial pre-decompression concentration of 2.5 mg/dl to 1.5 mg/dl 24 h post-decompression ( P <0.001). There was no association between SDMA concentration at initial presentation and SDMA concentration 5–20 days after urethral catheterization (Spearman’s ρ = 0.205, P = 0.314). Creatinine concentration upon initial presentation was associated with the 5–20 day values after urethral catheterization (Spearman’s ρ = 0.583, P <0.002). Twenty percent of cases were excluded due to bacterial growth on initial urine culture. SDMA and creatinine concentrations were significantly higher in these cases (median 59 µg/dl and 10.9 mg/dl, respectively) compared with those with negative cultures (median 14 µg/dl and 1.6 mg/dl [ P <0.002 and P <0.001], respectively). Conclusions and relevance Both SDMA and creatinine decreased significantly after urethral catheterization, suggesting that renal function post-decompression cannot be predicted by the pre-decompression concentrations of these values.

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