scholarly journals Babesia gibsoni cytochrome b mutations in canine blood samples submitted to a US veterinary diagnostic laboratory

2018 ◽  
Vol 32 (6) ◽  
pp. 1965-1969 ◽  
Author(s):  
Adam J. Birkenheuer ◽  
Henry S. Marr ◽  
James M. Wilson ◽  
Edward B. Breitschwerdt ◽  
Barbara A. Qurollo
Author(s):  
P.T. Matjila ◽  
B.L. Penzhorn ◽  
A.L. Leisewitz ◽  
R. Bhoora ◽  
R. Barker

Canine babesiosis caused by Babesia gibsoni was diagnosed in a 3-month-old Pit-bull pup during a routine clinical examination. Diagnosis was confirmed by way of smear examination, PCR, Reverse Line Blot (RLB) and sequence analysis which showed 100% homology with B. gibsoni (Japan AB118032) and Babesia sp. (Oklahoma) (AF205636). Haematology showed moderate anaemia and severe thrombocytopenia. Treatment was initiated with diminazene aceturate (Berenil RTU(R) followed by 2 doses of imidocarb diproprionate (Forray-65(R) 3 days and 14 days later, respectively. Babesia gibsoni DNA was still detectable 2 weeks post-treatment on the PCR/RLB test. A 10-day course of combination drug therapy using atovaquone and azithromycin was initiated. Blood samples taken on Day 1 and Day 40 after completion of treatment were negative for B. gibsoni DNA on PCR/RLB test. The implications of a possible introduction of B. gibsoni into South Africa are discussed.


1969 ◽  
Vol 67 (3) ◽  
pp. 409-416 ◽  
Author(s):  
D. G. McDevitt ◽  
W. J. McCaughey

SUMMARYA serological survey for brucella antibodies has been carried out in Northern Ireland in an attempt to assess the effects of the brucellosis eradication programme in cattle which has been in progress since 1959. One thousand eight hundred and ninety four sera from blood donors, from farmer's lung tests, and from various groups occupationally exposed to cattle were examined by the serum agglutination test, the anti-human globulin (A.H.G.) (Coombs) test and the complement-fixation test.The incidence of brucellosis in Northern Ireland is now less than it was 20 years ago, but is similar to that reported in a recent survey in England. It now appears that the principal method of transmission of brucellosis in the province is by occupational exposure rather than by drinking infected milk. Though it may be too early to make a serological assessment of the effects on human infections of brucellosis eradication in cattle, this survey provides a basis for future evaluation.We thank Prof. O. L. Wade, Prof. P. C. Elmes and Dr W. R. Kerr for their encouragement and helpful advice; the Medical Officers of Health in Antrim, Armagh, Down and Fermanagh, Londonderry, and Tyrone, and in Belfast and Londonderry County Borough for help in obtaining blood samples; Dr M. C. Huth of the Northern Ireland Blood Transfusion Service, for sera; Dr D. W. R. Mackenzie and Miss H. E. Cairns for sera from the Mycology Diagnostic Laboratory; Dr J. H. Connolly for sera from the Virus Reference Laboratory, and for helpful advice; the farm workers and veterinary surgeons for volunteering blood samples; the technical staff of the Veterinary Research Laboratories for carrying out the tests.


2020 ◽  
Author(s):  
Quincie Sipin ◽  
Farina Mustaffa Kamal ◽  
Malaika Watanabe ◽  
Puteri Azaziah Megat Abdul Rani ◽  
Nur Mahiza Md ◽  
...  

Abstract Background Canine tick-borne haemopathogens (TBH) constitute a significant concern worldwide. The detection of these TBH is mainly achieved by microscopic evaluation, seroprevalence, and molecular detection. The present study was designed to investigate the molecular detection of Anaplasma platys , Babesia gibsoni, Babesia vogeli , and Ehrlichia canis in shelter dogs and Rhipicephalus sanguineus ( sensu lato ) ticks infesting them in Malaysia.Results A total of 220 blood samples and 140 ticks were collected from 10 animal shelters in Peninsular Malaysia. The presence of haemopathogens was detected using conventional PCR, sequenced, and identified at the species level. Of the 220 blood samples, 77 (35%) were positive with at least one of the four haemopathogens, of which E. canis predominated (20%) followed by B. gibsoni and B. vogeli both with detection of 7%, and A. platys (12%). In tick samples, 5 (3.57%) of the collected samples were positive with at least one of the three haemopathogens. Low detection of E. canis and A. platys were present (n = 2; 1.43%) as well as B. vogeli shows only 1 sample positive (0.71%) and no detection of B. gibsoni . For co-infection in dogs, single infection is common (24%), while co-infection with two haemopathogens (10%) was also observed. The occurrence of infection with three TBH was also observed in the sampled dogs (1%).Conclusion E. canis is the most common TBH affecting shelter dogs in Peninsular Malaysia. Co-infection is quite common and the most common co-infection present was E. canis and A. platys. The study highlighted the first extensive molecular detection of TBH in dogs and R. sanguineus ( sensu lato ) ticks in Malaysia


2010 ◽  
Vol 4 (03) ◽  
pp. 139-143 ◽  
Author(s):  
Smita Benjamin Shekatkar ◽  
Belgode Narasimha Harish ◽  
Godfred Antony Menezes ◽  
Subhash Chandra Parija

Background: Leptospirosis is a zoonotic disease with a worldwide distribution. There is a paucity of available data about prevalence of this disease in Pondicherry. Our aim was to investigate the seropositivity rate of leptospirosis in suspected cases and also to identify the predominant serogroups present by performing Microscopic Agglutination Test (MAT). The other aim of this study was to compare the results of a commercially available IgM ELISA with that of MAT. Methodology: A total of 110 blood samples from patients suspected of leptospirosis were sent for diagnosis. These samples were subjected to IgM ELISA and the microscopic agglutination test (MAT). MAT was done using a panel of 12 Leptospira serovars. Results: MAT analysis of the 110 samples showed 40 (36%) to be positive. Antibodies were predominantly seen against serogroup Leptospira Icterohemorrhagiae (27%), followed by Pomona (17%), and Pyrogenes (12%). IgM ELISA done on these samples showed a positivity of 37% compared to MAT. Conclusion: This study reveals that the MAT test can be standardized in a diagnostic laboratory and used in conjunction with an IgM ELISA.


2013 ◽  
Vol 10 (2) ◽  
pp. 249-254 ◽  
Author(s):  
MH Talukder ◽  
A Matsuu ◽  
A Iguchi ◽  
BC Roy ◽  
N Nishii ◽  
...  

To identify tick-borne pathogens in blood samples from 68 dogs were examined for Babesia, Anaplasma/Ehrichia, Mycoplasma, and Hepatozoon spp. by using a molecular tool. The detection of DNA was performed using specific primers for partial 16S rRNA or 18S rRNA sequence, and amplicon sequences were analyzed. Our investigation detected Babesia gibsoni (26 dogs) and Anaplasma sp. AnHl446 (2 dogs), Mycoplasma haemocanis (27 dogs), and an unknown Mycoplasma spp were detected (2 dogs), which was most closely related to Candidatus Mycoplasma turicensis, on the basis of phylogenetic analysis. Hepatozoon DNA could not be detected in this study.DOI: http://dx.doi.org/10.3329/jbau.v10i2.14915 J. Bangladesh Agril. Univ. 10(2): 249-253, 2012


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