scholarly journals Clinical Assessment of a Point‐of‐Care Serum Amyloid A Assay in Foals with Bronchopneumonia

2016 ◽  
Vol 30 (4) ◽  
pp. 1338-1343 ◽  
Author(s):  
S. Giguère ◽  
L.J. Berghaus ◽  
C.D. Miller
Keyword(s):  
Clinical Assessment ◽  
Serum Amyloid A ◽  
Point Of Care ◽  
Serum Amyloid ◽  
Amyloid A

scholarly journals Whole-Blood Validation of a New Point-of-care Equine Serum Amyloid A Assay

2020 ◽  
Vol 94 ◽  
pp. 103222
Author(s):  
Bruno Karam ◽  
Siddra Hines ◽  
Lauren Skipper ◽  
Nicola Pusterla
Keyword(s):  
Whole Blood ◽  
Serum Amyloid A ◽  
Point Of Care ◽  
Serum Amyloid ◽  
Amyloid A

scholarly journals Analytical Validation of Two Point-of-Care Assays for Serum Amyloid A Measurements in Cats

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2518
Author(s):  
Damián Escribano ◽  
Alba Ortín Bustillo ◽  
Luis Pardo Marín ◽  
Andrea Navarro Rabasco ◽  
Pablo Ruiz Herrera ◽  
...  
Keyword(s):  
Gold Standard ◽  
Serum Amyloid A ◽  
Performance Test ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Serum Amyloid ◽  
Analytical Validation ◽  
Serum Samples ◽  
Amyloid A

Serum Amyloid A (SAA) is one of the most sensitive tests to detect inflammation in cats. In this study, two point-of-care assays for SAA measurements in cats (FUJI DRI-CHEM IMMUNO AU CARTRIDGE vf-SAA (method A), and CUBE-VET analyser (Method B), were analytically evaluated. Regarding the imprecision precision only the method A showed intra-assay and inter-assay CV < 10% at all concentrations. Both assays showed linearity with r close to 1 and the recovery were in the range of 81–112% for assay A and 85–125% for assay B and the limit of detection were 3.75 and 0.5 mg/dL for method A and B, respectively. A previously validated method for SAA quantification SAATIA; LZ-SAA (method C) was used as gold-standard to evaluate the accuracy of the assays. Significant correlations (p < 0.0001) were found between assays A and C (r = 0.94) and B and C (r = 0.91). In addition, an overlap performance test was made using serum samples from cats with non-inflammatory and cats with inflammatory. Both assays showed higher median SAA concentrations in cats with inflammatory diseases than in cats without inflammatory diseases (p < 0.0001). In conclusion, this manuscript provides data about the possible application of two point-of-care assays for the measurement of SAA concentration in cats.

Comparison of feline serum amyloid A (SAA) measurements assessed by a point-of-care test analyzer to a validated immunoturbidimetric method

2017 ◽  
Vol 27 (2) ◽  
pp. 321-325 ◽  
Author(s):  
Elodie Gaillard ◽  
Marcel Aumann ◽  
Vincent Leynaud ◽  
Jean-Pierre Braun ◽  
Catherine Trumel
Keyword(s):  
Serum Amyloid A ◽  
Point Of Care ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Point Of Care Test

scholarly journals Serum Amyloid A and Lipoprotein Associated Phospholipase A2 Levels in Patients with Malign Melanoma: Correlations with Clinical Assessment and Stage

2018 ◽  
Vol 12 (3) ◽  
pp. 135-142
Author(s):  
Yavuz Kayaş ◽  
Ferhan Sağın ◽  
Yasemin Akçay ◽  
Gizem Kocabaş Yenipazar ◽  
Elif Azarsız ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Clinical Assessment ◽  
Serum Amyloid A ◽  
Serum Amyloid ◽  
Amyloid A

scholarly journals Comparison of a point-of-care serum amyloid A analyzer frequently used in equine practice with 2 turbidimetric immunoassays used in human and veterinary medicine

2021 ◽  
pp. 104063872110560
Author(s):  
Julia Kiemle ◽  
Sarah Hindenberg ◽  
Natali Bauer ◽  
Michael Roecken
Keyword(s):  
Serum Amyloid A ◽  
Point Of Care ◽  
Method Comparison ◽  
Serum Amyloid ◽  
Serum Samples ◽  
Amyloid A ◽  
Hook Effect ◽  
Significant Difference ◽  
Median Period ◽  
Patient Side

Rapid, accurate detection of serum amyloid A (SAA) is needed in equine practice. We validated a patient-side point-of-care (POC) assay (Stablelab; Zoetis) compared to the turbidimetric immunoassays LZ-SAA (TIA-Hum) and VET-SAA (TIA-Vet; both Eiken Chemical). Analytical performance was assessed at 3 different concentration ranges and with interferences. Inter-method comparison using 49 equine serum samples revealed a significant difference between median SAA results ( p < 0.0001), with the strongest bias between the POC and TIA-Vet (median 1,093 vs. 578 mg/L). The median SAA value obtained with the TIA-Hum method was 752 mg/L. Correlation between POC/TIA-Hum and between POC/TIA-Vet was fair (rs = 0.77 and 0.69) and excellent between both TIAs (rs = 0.93). Bias between POC/TIA-Hum, POC/TIA-Vet, and TIA-Hum/TIA-Vet was −56.7%, –80.9%, and −28.2%, respectively. POC intra- and inter-assay CVs (16.1–30% and 19.8–35.5%) were higher than TIA CVs (generally <12%). Bilirubin and hemoglobin had a negative bias on POC and TIA-Vet results (−16.6 to −45.6%); addition of intralipid yielded a positive bias (35.9–77.4%). The POC had good linearity of SAA concentrations up to 10,312 mg/L ( R2 = 0.92). A hook effect was present at SAA >3,000 mg/L for the POC assay. Equine serum SAA was stable over a median period of 2.5 y when stored at −80°C. Overall, there was excellent-to-moderate correlation between tests, but imprecision and hook effect of the POC, as well as bias between the methods, must be considered.

scholarly journals Analytical validation of a new point‐of‐care assay for serum amyloid A in horses

2018 ◽  
Vol 50 (5) ◽  
pp. 678-683 ◽  
Author(s):  
D. Schwartz ◽  
N. Pusterla ◽  
S. Jacobsen ◽  
M. M. Christopher
Keyword(s):  
Serum Amyloid A ◽  
Point Of Care ◽  
Serum Amyloid ◽  
Analytical Validation ◽  
Amyloid A ◽  
Point Of Care Assay

Serum Amyloid A in Healthy Female Bottlenose Dolphins (Tursiops truncatus) During and After Uncomplicated Pregnancy

2017 ◽  
Vol 43 (4) ◽  
pp. 417-420 ◽  
Author(s):  
Sarah N. Miller ◽  
Michelle Davis ◽  
Jorge A. Hernandez ◽  
Judy St. Leger ◽  
Carolyn Cray ◽  
...  
Keyword(s):  
Tursiops Truncatus ◽  
Serum Amyloid A ◽  
Bottlenose Dolphins ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Uncomplicated Pregnancy ◽  
Healthy Female
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