scholarly journals Analytical validation of a new point‐of‐care assay for serum amyloid A in horses

2018 ◽  
Vol 50 (5) ◽  
pp. 678-683 ◽  
Author(s):  
D. Schwartz ◽  
N. Pusterla ◽  
S. Jacobsen ◽  
M. M. Christopher
Keyword(s):  
Serum Amyloid A ◽  
Point Of Care ◽  
Serum Amyloid ◽  
Analytical Validation ◽  
Amyloid A ◽  
Point Of Care Assay

scholarly journals Analytical Validation of Two Point-of-Care Assays for Serum Amyloid A Measurements in Cats

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2518
Author(s):  
Damián Escribano ◽  
Alba Ortín Bustillo ◽  
Luis Pardo Marín ◽  
Andrea Navarro Rabasco ◽  
Pablo Ruiz Herrera ◽  
...  
Keyword(s):  
Gold Standard ◽  
Serum Amyloid A ◽  
Performance Test ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Serum Amyloid ◽  
Analytical Validation ◽  
Serum Samples ◽  
Amyloid A

Serum Amyloid A (SAA) is one of the most sensitive tests to detect inflammation in cats. In this study, two point-of-care assays for SAA measurements in cats (FUJI DRI-CHEM IMMUNO AU CARTRIDGE vf-SAA (method A), and CUBE-VET analyser (Method B), were analytically evaluated. Regarding the imprecision precision only the method A showed intra-assay and inter-assay CV < 10% at all concentrations. Both assays showed linearity with r close to 1 and the recovery were in the range of 81–112% for assay A and 85–125% for assay B and the limit of detection were 3.75 and 0.5 mg/dL for method A and B, respectively. A previously validated method for SAA quantification SAATIA; LZ-SAA (method C) was used as gold-standard to evaluate the accuracy of the assays. Significant correlations (p < 0.0001) were found between assays A and C (r = 0.94) and B and C (r = 0.91). In addition, an overlap performance test was made using serum samples from cats with non-inflammatory and cats with inflammatory. Both assays showed higher median SAA concentrations in cats with inflammatory diseases than in cats without inflammatory diseases (p < 0.0001). In conclusion, this manuscript provides data about the possible application of two point-of-care assays for the measurement of SAA concentration in cats.

scholarly journals Whole-Blood Validation of a New Point-of-care Equine Serum Amyloid A Assay

2020 ◽  
Vol 94 ◽  
pp. 103222
Author(s):  
Bruno Karam ◽  
Siddra Hines ◽  
Lauren Skipper ◽  
Nicola Pusterla
Keyword(s):  
Whole Blood ◽  
Serum Amyloid A ◽  
Point Of Care ◽  
Serum Amyloid ◽  
Amyloid A

scholarly journals Serum paraoxonase 1 activity in cats: analytical validation, reference intervals, and correlation with serum amyloid A and alpha-1-acid glycoprotein

2020 ◽  
Vol 32 (6) ◽  
pp. 844-855
Author(s):  
Gabriele Rossi ◽  
Sara Meazzi ◽  
Alessia Giordano ◽  
Saverio Paltrinieri
Keyword(s):  
Acute Phase ◽  
Room Temperature ◽  
Serum Amyloid A ◽  
Serum Amyloid ◽  
Paraoxonase 1 ◽  
Biological Behavior ◽  
Pon1 Activity ◽  
Analytical Validation ◽  
Acid Glycoprotein ◽  
Amyloid A

Paraoxonase 1 (PON1) is an inflammation marker associated with lipid oxidation and is used as a diagnostic marker in people. There is no information about the suitable substrate and analytic performance in cats, or its biological behavior compared with other inflammation markers. Our aims were to validate a paraoxon-based method to measure PON1 activity in feline serum, to assess stability of PON1 under different storage conditions and the impact of interfering elements, to determine a reference interval (RI) for healthy cats, and to correlate PON1 activity with 2 major acute-phase proteins. Intra- and inter-assay precision, accuracy, and RI were assessed using fresh serum. The same specimens were stored at room temperature, refrigerated, or frozen, and retested at defined intervals. Hemolysis, lipemia, and icterus were simulated to study interferences. PON1 results were compared to serum amyloid A (SAA) and alpha-1-acid glycoprotein (AGP) results. Analytical validation yielded precise and accurate results. PON1 activity is stable for up to 24 h at room temperature and up to 48 h at 4°C. Freezing at −20°C results in an increase after 72 h, with return to baseline values after 1 wk, that again increases after 6 mo. Only hyperlipemia interfered with PON1 activity. The RI based on 71 healthy cats was 58–154 U/L. PON1 activity was negatively correlated with AGP, but not with SAA. Serum PON1 activity can be measured accurately in cats, and it acts as a negative acute-phase protein.

Comparison of feline serum amyloid A (SAA) measurements assessed by a point-of-care test analyzer to a validated immunoturbidimetric method

2017 ◽  
Vol 27 (2) ◽  
pp. 321-325 ◽  
Author(s):  
Elodie Gaillard ◽  
Marcel Aumann ◽  
Vincent Leynaud ◽  
Jean-Pierre Braun ◽  
Catherine Trumel
Keyword(s):  
Serum Amyloid A ◽  
Point Of Care ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Point Of Care Test

scholarly journals Comparison of a point-of-care serum amyloid A analyzer frequently used in equine practice with 2 turbidimetric immunoassays used in human and veterinary medicine

2021 ◽  
pp. 104063872110560
Author(s):  
Julia Kiemle ◽  
Sarah Hindenberg ◽  
Natali Bauer ◽  
Michael Roecken
Keyword(s):  
Serum Amyloid A ◽  
Point Of Care ◽  
Method Comparison ◽  
Serum Amyloid ◽  
Serum Samples ◽  
Amyloid A ◽  
Hook Effect ◽  
Significant Difference ◽  
Median Period ◽  
Patient Side

Rapid, accurate detection of serum amyloid A (SAA) is needed in equine practice. We validated a patient-side point-of-care (POC) assay (Stablelab; Zoetis) compared to the turbidimetric immunoassays LZ-SAA (TIA-Hum) and VET-SAA (TIA-Vet; both Eiken Chemical). Analytical performance was assessed at 3 different concentration ranges and with interferences. Inter-method comparison using 49 equine serum samples revealed a significant difference between median SAA results ( p < 0.0001), with the strongest bias between the POC and TIA-Vet (median 1,093 vs. 578 mg/L). The median SAA value obtained with the TIA-Hum method was 752 mg/L. Correlation between POC/TIA-Hum and between POC/TIA-Vet was fair (rs = 0.77 and 0.69) and excellent between both TIAs (rs = 0.93). Bias between POC/TIA-Hum, POC/TIA-Vet, and TIA-Hum/TIA-Vet was −56.7%, –80.9%, and −28.2%, respectively. POC intra- and inter-assay CVs (16.1–30% and 19.8–35.5%) were higher than TIA CVs (generally <12%). Bilirubin and hemoglobin had a negative bias on POC and TIA-Vet results (−16.6 to −45.6%); addition of intralipid yielded a positive bias (35.9–77.4%). The POC had good linearity of SAA concentrations up to 10,312 mg/L ( R2 = 0.92). A hook effect was present at SAA >3,000 mg/L for the POC assay. Equine serum SAA was stable over a median period of 2.5 y when stored at −80°C. Overall, there was excellent-to-moderate correlation between tests, but imprecision and hook effect of the POC, as well as bias between the methods, must be considered.

Serum Amyloid A in Healthy Female Bottlenose Dolphins (Tursiops truncatus) During and After Uncomplicated Pregnancy

2017 ◽  
Vol 43 (4) ◽  
pp. 417-420 ◽  
Author(s):  
Sarah N. Miller ◽  
Michelle Davis ◽  
Jorge A. Hernandez ◽  
Judy St. Leger ◽  
Carolyn Cray ◽  
...  
Keyword(s):  
Tursiops Truncatus ◽  
Serum Amyloid A ◽  
Bottlenose Dolphins ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Uncomplicated Pregnancy ◽  
Healthy Female
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