The effects of biodegradable poly(lactic-co-glycolic acid)-based microspheres loaded with quercetin on stemness, viability and osteogenic differentiation potential of stem cell spheroids

2018 ◽  
Vol 53 (5) ◽  
pp. 801-815 ◽  
Author(s):  
H. Lee ◽  
T. T. Nguyen ◽  
M. Kim ◽  
J.-H. Jeong ◽  
J.-B. Park
Keyword(s):  
Stem Cell ◽  
Osteogenic Differentiation ◽  
Glycolic Acid ◽  
Differentiation Potential ◽  
Cell Spheroids ◽  
Biodegradable Poly

scholarly journals The Osteogenic Capacity of HumanAmniotic Membrane Mesenchymal Stem Cell (hAMSC) and Potential for Application in Maxillofacial Bone Reconstruction in Vitro Study

2020 ◽  
Vol 4 (1) ◽  
pp. 17
Author(s):  
David Kamadjaja
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Amniotic Membrane ◽  
Isolation Procedure ◽  
Target Cells ◽  
Human Amniotic Membrane ◽  
Bone Reconstruction ◽  
Differentiation Potential

Amniotic membrane of human placenta is a source of abundant mesenchymal stem cell (hAMSC) which makes it a potential source of allogeneic multipotent cell for bone healing.  However, much has to be explored about its isolation procedure and the osteogenic differentiation potential. The aims of this study are to establish the procurement procedure of human amniotic membrane, the isolation   and culture   of hAMSC, the MSC phenotypic characterization, and the in vitro osteogenic differentiation of hAMSC.  Results of the study are as follows. The quality of human amniotic membrane would be best if procured from Caesarean operation under highly aseptic condition to avoid fungal and bacterial contamination on the culture.  Isolation procedure using modified   Soncini protocol yielded large amount of MSC with high proliferative capacity in culture medium.  Characterization of hAMSC showed that the majority of the target cells exhibited specific MSC markers (CD10S and CD90) with a small number of these cells expressing CD45the marker of hematopoeitic cells. The in vitro osteogenic differentiation of hAMSC  followed by Alizarin  Red staining showed that  osteoblastic differentiation  was  detected in a significantly   high  number  of cells.  This study concludes that hAMSCs isolated from human amniotic membrane have the capacity for in vitro osteogenesis which makes them be one of the potential allogeneic stem cells for application in maxillofacial bone reconstruction.

scholarly journals Osteogenic Differentiation of Bone Marrow Stem Cell in Poly(Lactic-co-Glycolic Acid) Scaffold Loaded Various Ratio of Hydroxyapatite

2013 ◽  
Vol 6 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Hyeongseok Kim ◽  
Hye Min Kim ◽  
Ji Eun Jang ◽  
Cho Min Kim ◽  
Eun Young Kim ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Osteogenic Differentiation ◽  
Glycolic Acid ◽  
Bone Marrow Stem Cell ◽  
Marrow Stem Cell

scholarly journals The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression

2021 ◽  
Vol 12 ◽  
Author(s):  
Ding Li ◽  
Qi Yuan ◽  
Liang Xiong ◽  
Aoyu Li ◽  
Yu Xia
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Critical Role ◽  
Osteoporotic Bone ◽  
Nodule Formation ◽  
Heterotopic Bone ◽  
Heterotopic Bone Formation ◽  
Differentiation Potential

Osteoporosis is a complex multifactorial disorder linked to various risk factors and medical conditions. Bone marrow-derived mesenchymal stem cell (BMSC) dysfunction potentially plays a critical role in osteoporosis pathogenesis. Herein, the study identified that miR-4739 was upregulated in BMSC cultures harvested from osteoporotic subjects. BMSCs were isolated from normal and osteoporotic bone marrow tissues and identified for their osteogenic differentiation potential. In osteoporotic BMSCs, miR-4739 overexpression significantly inhibited cell viability, osteoblast differentiation, mineralized nodule formation, and heterotopic bone formation, whereas miR-4739 inhibition exerted opposite effects. Through direct binding, miR-4739 inhibited distal-less homeobox 3 (DLX3) expression. In osteoporotic BMSCs, DLX3 knockdown also inhibited BMSC viability and osteogenic differentiation. Moreover, DLX3 knockdown partially attenuated the effects of miR-4739 inhibition upon BMSCs. Altogether, the miR-4739/DLX3 axis modulates the capacity of BMSCs to differentiate into osteoblasts, which potentially plays a role in osteoporosis pathogenesis. The in vivo and clinical functions of the miR-4739/DLX3 axis require further investigation.

scholarly journals NELL-1 Increased the Osteogenic Differentiation and mRNA Expression of Spheroids Composed of Stem Cells

Medicina ◽  
2021 ◽  
Vol 57 (6) ◽  
pp. 586
Author(s):  
Jong-Ho Lee ◽  
Young-Min Song ◽  
Sae-Kyung Min ◽  
Hyun-Jin Lee ◽  
Hye-Lim Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Alkaline Phosphatase ◽  
Stem Cell ◽  
Cell Viability ◽  
Osteogenic Differentiation ◽  
Mrna Expression ◽  
Cell Counting ◽  
Calcium Deposition ◽  
Anthraquinone Dye ◽  
Cell Spheroids

Background and objectives: NELL-1 is a competent growth factor and it reported to target cells committed to the osteochondral lineage. The secreted, osteoinductive glycoproteins are reported to rheostatically control skeletal ossification. This study was performed to determine the effects of NELL-1 on spheroid morphology and cell viability and the promotion of osteogenic differentiation of stem cell spheroids. Materials and Methods: Cultures of stem cell spheroids of gingiva-derived stem cells were grown in the presence of NELL-1 at concentrations of 1, 10, 100, and 500 ng/mL. Evaluations of cell morphology were performed using a microscope, and cell viability was assessed using a two-color assay and Cell Counting Kit-8. Evaluation of the activity of alkaline phosphatase and calcium deposition assays involved anthraquinone dye assay to determine the level of osteogenic differentiation of cell spheroids treated with NELL-1. Real-time quantitative polymerase chain reaction (qPCR) was used to evaluate the expressions of RUNX2, BSP, OCN, COL1A1, and β-actin mRNAs. Results: The applied stem cells produced well-formed spheroids, and the addition of NELL-1 at tested concentrations did not show any apparent changes in spheroid shape. There were no significant changes in diameter with addition of NELL-1 at 0, 1, 10, 100, and 500 ng/mL concentrations. The quantitative cell viability results derived on Days 1, 3, and 7 did not show significant disparities among groups (p > 0.05). There was statistically higher alkaline phosphatase activity in the 10 ng/mL group compared with the unloaded control on Day 7 (p < 0.05). A significant increase in anthraquinone dye staining was observed with the addition of NELL-1, and the highest value was noted at 10 ng/mL (p < 0.05). qPCR results demonstrated that the mRNA expression levels of RUNX2 and BSP were significantly increased when NELL-1 was added to the culture. Conclusions: Based on these findings, we conclude that NELL-1 can be applied for increased osteogenic differentiation of stem cell spheroids.

scholarly journals Human Gingival Fibroblast: Single Colonies vs. Heterogeneous Culture, Characterization and Osteogenic Differentiation

2018 ◽  
Vol 2 (1) ◽  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Osteogenic Differentiation ◽  
Gingival Tissue ◽  
Osteogenic Potential ◽  
Stem Cell Population ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Differentiation Potential ◽  
Dental Tissues

Cells with mesenchymal stem cell (MSC) properties were successfully isolated and characterized from different dental tissues. Amongst these cells are the human gingival fibroblast cells (GFs). Their use in tissue engineering is promising. However, choosing the right cellular population is one of several factors that are necessary for a successful tissue engineering approach. In order to better choose which population of cells to use, we isolated the GFs single colonies. We identified them, osteogenically induced them and compared them to the heterogeneous culture of these cells. Materials and methods: GFs cells were extracted from human gingival tissue; incubated to confluency. After which they were counted, serially diluted and seeded in 6 well plates. The cells were observed daily to locate the first formed colonies. Borosilicate cylinders were used to pick up the colonies. Flow cytometry was used to identify Stem cells surface markers to compare single colonies and heterogeneous cultures. The cells were then osteogenically induced for 21 days. The following assays were performed to compare the osteogenic potential between single colonies and heterogeneous cultures; Calcium assay, ALP/DNA specific activity, RT-qPCR for osteogenic related genes (OPN, OCN, ALP) and western blot analysis. Results: All the assays results were consistent in revealing an increased osteogenic differentiation potential of the heterogeneous culture of the GFs over the single colonies cultures. These results indicate that the heterogeneous cultures of GFs have a higher stem cell population and subsequent osteogenic differentiation potential than the single cell colonies’ cultures.

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