Engineering a Freestanding Biomimetic Cardiac Patch Using Biodegradable Poly(lactic-co-glycolic acid) (PLGA) and Human Embryonic Stem Cell-derived Ventricular Cardiomyocytes (hESC-VCMs)

2014 ◽  
Vol 15 (3) ◽  
pp. 426-436 ◽  
Author(s):  
Yin Chen ◽  
Junping Wang ◽  
Bo Shen ◽  
Camie W. Y. Chan ◽  
Chaoyi Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Glycolic Acid ◽  
Embryonic Stem ◽  
Ventricular Cardiomyocytes ◽  
Biodegradable Poly ◽  
Cardiac Patch

scholarly journals Morphometric Analysis of Human Embryonic Stem Cell-Derived Ventricular Cardiomyocytes: Determining the Maturation State of a Population by Quantifying Parameters in Individual Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Harvey Y. S. Chan ◽  
Wendy Keung ◽  
Ronald A. Li ◽  
Andrew L. Miller ◽  
Sarah E. Webb
Keyword(s):  
Stem Cell ◽  
Oleic Acid ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Quantitative Methods ◽  
Embryonic Stem ◽  
Ventricular Cardiomyocytes ◽  
Morphometric Methods ◽  
Maturation State

Quantitative methods were established to determine the level of maturation of human embryonic stem cell-derived ventricular cardiomyocytes (hESC-vCMs) that were treated with different metabolic stimulants (i.e., isoproterenol and oleic acid) during early differentiation. Cells were double-immunolabeled withα-actinin and COX IV antibodies, to label the myofibrils and mitochondria, respectively, after which images were acquired via confocal microscopy. In order to determine the extent of differentiation, image analysis protocols were then used to quantify cell shape and area, as well as the degree of myofibrillar organization and intercalation of mitochondria between the myofibrils within the cells. We demonstrated that oleic acid or isoproterenol alone, or a combination of the two, induced a more elongated hESC-vCM phenotype than the untreated controls. In addition, cells treated with isoproterenol alone exhibited a similar level of myofibrillar organization as the controls, but those treated with oleic acid with/without isoproterenol exhibited a more organized (parallel) orientation of myofibrils. The combined isoproterenol/oleic acid treatment also resulted in enhanced intercalation of mitochondria between the myofibrils. We suggest that these quantitative morphometric methods might serve as simple and effective tools that can be utilized in the determination of the level of structural maturation of hESC-vCMs.

IGF1-mediated human embryonic stem cell self-renewal recapitulates the embryonic niche

2020 ◽  
Author(s):  
Wamaitha SE ◽  
Grybel KJ ◽  
Alanis-Lobato G ◽  
Gerri C ◽  
Ogushi S ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Self Renewal
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