Development of livestock feed additives using porcine blood cells

2021 ◽  
Author(s):  
Jae‐Hwan Choi ◽  
Tae‐Hwan Jung ◽  
Hyeon‐Ju Seo ◽  
Kyoung‐Sik Han
Keyword(s):  
Blood Cells ◽  
Feed Additives ◽  
Livestock Feed ◽  
Porcine Blood

scholarly journals PSVI-37 Effects of deoxynivalenol (DON) and selected feed additives on viability and tight junction integrity of IPEC-J2 cells

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 202-202
Author(s):  
Hang Lu ◽  
Kolapo Ajuwon
Keyword(s):  
Tight Junction ◽  
Feed Additives ◽  
Control Treatment ◽  
Epithelial Cell Line ◽  
Intestinal Epithelial ◽  
Negative Effects ◽  
Semipermeable Membranes ◽  
Livestock Feed ◽  
Epithelial Tight Junction ◽  
Porcine Intestinal Epithelial Cell

Abstract Intestinal epithelial cells represent the frontline of host defense against invasion by enteric pathogens and uptake of potentially harmful substances. Mycotoxins are deleterious feed contaminants routinely found in cereal grains used in livestock feed. Development of strategies to eliminate mycotoxins and mitigate their harmful effects on the animal is of major importance to the livestock industry. The objective of the current study was to determine the effects of deoxynivalenol (DON), a common mycotoxin contaminant, and selected prebiotic, probiotic, and essential oil blended feed additives, on viability and integrity in porcine intestinal epithelial cell line (IPEC-J2). IPEC-J2 cells were grown on either plastic or semipermeable membranes until 100% confluence and treated with DON (12.5 uM) with or without additives (0.01 mg/mL Biolex® and 0.0001 mg/mL Encinnate®, Biomatrix Inc.) Use of DON at 12.5 uM decreased the trans-epithelial electrical resistance (TEER) by 37.5% compared to control, and led to 88.7% increase in FITC-dextran permeability after 3 days of culture, but with no negative effects on cell viability. The decrease in TEER by DON was minimized in the presence of Biolex (22.2%) and Encinnate (20.5%) relative to control. Treatment with DON also decreased the mRNA (24.0%) and protein abundance (64.4%) of claudin 3 and this was partially reversed by the additives, 11.5 and 11.0% decrease for claudin 3 mRNA and 58.7 and 56.7% decrease for the protein relative to control for Biolex and Encinnate, respectively. In summary, DON led to impaired epithelial tight junction integrity and this was partially rescued by the additives tested.

scholarly journals Ethological and biological features of the organism of the black-and-white bulls when using natural feed additives in the diets

2020 ◽  
Vol 17 ◽  
pp. 00237
Author(s):  
Oksana Krasnova ◽  
Ekaterina Hardina ◽  
Sergei Hramov ◽  
Michail Kudrin ◽  
Nina Konik
Keyword(s):  
Active Substance ◽  
Feed Additives ◽  
Biologically Active ◽  
Feed Additive ◽  
Biologically Active Substance ◽  
Black And White ◽  
Scientific Experiment ◽  
Biological Features ◽  
Livestock Feed ◽  
Meat Productivity

The authors studied the natural feed additive effect to the behaviour and biochemical blood composition of the black-and-white bulls. As results of the scientific experiment we came to the conclusion that using of the biologically active substance dihydroquercetin as a part of a natural feed additive in livestock feed for the metabolic processes intensity stimulation, the nutrient synthesis intensity and higher rates meat productivity formation.

scholarly journals Study of Hematological Characteristics and Mineral Metabolism of Moscow Black Chickens in the Process of Acclimatization in the Astrakhan Region

2022 ◽  
Vol 16 ◽  
pp. 9-13
Author(s):  
Bratashova Tatyana Sergeevna ◽  
Zakharkina Natalya Ivanovna ◽  
Shcherbakova Elena Nikolaevna ◽  
Safonov Vladimir Aleksandrovich
Keyword(s):  
Trace Elements ◽  
Blood Cells ◽  
Mineral Metabolism ◽  
White Blood Cells ◽  
Climatic Conditions ◽  
Feed Additives ◽  
Moscow Region ◽  
Lower Volga Region ◽  
Low Levels ◽  
Hematological And Biochemical Parameters

The study of indicators characterizing the processes associated with the acclimatization of birds in new biogeochemical conditions is of interest in connection with the prospects of the poultry industry in the Astrakhan Region, where the lack of important trace elements in soils and plant feeds: Se, I, Co, is observed. In the presented study, the main hematological and biochemical parameters of blood and mineral metabolism were determined in 2 groups of chickens of the Moscow black breed: imported to the Astrakhan Region from Moscow and kept in the Moscow Region. The analyses revealed strong differences (P<0.01) in indicators of two groups. The acclimatized birds were characterized by low levels of red blood cells and hemoglobin in the blood, as well as a higher content of white blood cells than in the second group. The chickens had impaired lipid and protein metabolism. A 71.3% increase in the level of total sugar most likely indicated a stress state of birds. Compared with the chickens in the Moscow Region, the level of calcium in their blood was 32.4% lower, phosphorus – 12.9%. To a large extent, a decrease in the levels of iodine and selenium – by 5.3 and 5.2 times, respectively, was observed. Thus, in the absence of external changes, acclimatized chickens are subject to a latent form of combined I- and Se-hypomicroelementosis. At the same time, a drop in egg productivity by 19% is the recorded fact. The necessity to adapt to the new climatic conditions of the Lower Volga region for birds, combined with a lack of important trace elements, can cause increased oxidative stress in acclimatized birds. Based on the results of the study, the use of feed additives that make up for the deficiency of necessary trace elements is recommended.

Heparin and EDTA as anticoagulant differentially affect cytokine mRNA level of cultured porcine blood cells

2007 ◽  
Vol 324 (1-2) ◽  
pp. 38-47 ◽  
Author(s):  
J.C. Duvigneau ◽  
W. Sipos ◽  
R.T. Hartl ◽  
M. Bayer ◽  
R. Moldzio ◽  
...  
Keyword(s):  
Blood Cells ◽  
Mrna Level ◽  
Cytokine Mrna ◽  
Porcine Blood ◽  
Cytokine Mrna Level

scholarly journals Efficacy of Phytogenic Feed Additive on Performance, Production and Health Status of Monogastric Animals – A Review

2017 ◽  
Vol 17 (4) ◽  
pp. 929-948 ◽  
Author(s):  
Santi Devi Upadhaya ◽  
In Ho Kim
Keyword(s):  
Health Status ◽  
Current Knowledge ◽  
Feed Additives ◽  
Feed Additive ◽  
Growth Promoter ◽  
Full Understanding ◽  
Plant Parts ◽  
Livestock Feed ◽  
Wide Range ◽  
Cold Pressed

AbstractThe growing concerns of consumers on the use of antibiotic as a growth promoter in livestock feed have fueled the interest in alternative products. In the recent years a group of natural products known as phytogenics has been a focus of several studies. Phytogenics are a heterogeneous group of feed additives originating from plants and consist of herbs, spices, fruit, and other plant parts. These feed additives are reported to have a wide range of activities including antimicrobial, anthelminthic, antioxidant, growth enhancer, and immune modulator. Besides these properties they are also reported to stimulate feed intake and endogenous secretion and enhance production. They include many different bio-active ingredients such as alkaloids, bitters, flavonoids, glycosides, mucilage, saponins, tannins phenolics, polyphenols, terpenoids, polypeptide, thymol, cineole, linalool, anethole, allicin, capsaicin, allylisothiocyanate, and piperine. These feed additives have been tested in the form of extracts, cold pressed oils, essential oils in a number of animals but the results are variable. Therefore, their application as feed additive has been limited, largely owing to their inconsistent efficacy and lack of full understanding of the modes of action. The future of these feed additives depend on the characteristics of herbs, the knowledge on their major and minor constituents, the in-depth knowledge on their mode of action and their value based on the safety to animal and their products. The aim of this review is to summarize on the current knowledge on the use of phytogenic as a feed additive in monogastric animals.

scholarly journals Organic farming: an ecofriendly way to sustainable agriculture

2007 ◽  
Vol 8 ◽  
pp. 115-118 ◽  
Author(s):  
Daya N. Mandal
Keyword(s):  
Organic Farming ◽  
Crop Rotation ◽  
Growth Regulators ◽  
Farming System ◽  
Feed Additives ◽  
Organic Manures ◽  
Organic Farm ◽  
Livestock Feed ◽  
Agriculture And Environment ◽  
Organic Farming System

Organic farm products have got more value as compared to inorganic products. People are becoming more conscious for their health and environment. Bio-farming is sustainable and eco-friendly which enables to conserve biodiversity and to protect environment. Organic farming system avoids or largely excludes the use of synthetically compounded fertilizers, pesticides, growth regulators, and livestock feed additives. It mainly relies upon crop rotation, organic manures, bio-pesticides and integrated pest management (IPM). In this paper emphasis has been given to practice in favors of organic farming, bio-pesticides, and preparation and use of organic manures by different methods in a hygienic manner. The Journal of AGRICULTURE AND ENVIRONMENT Vol. 8, 2007, pp. 115-118

scholarly journals Сorrection of sodium and potassium content in the blood of the cows with the aid of feed additives of Hermatsins taking into account typological features of the nervous

2020 ◽  
pp. 96-102
Author(s):  
E. Zhurenko
Keyword(s):  
Blood Cells ◽  
Weak Type ◽  
Nervous Activity ◽  
Sodium Content ◽  
Feed Additives ◽  
Potassium Content ◽  
Feed Additive ◽  
Feed Supplement ◽  
Higher Nervous Activity ◽  
Sodium And Potassium

The eff ectiveness of correction of the content of some macronutrients in the blood of cows with diff erent types of higher nervous activity (HNA) and vegetative status has been confi rmed using feed additive “Germatsink”. In cows with a weak type of higher nervous activity, the potassium content in the serum in 10 days after the administration of the nanopreparation increased by 16.0% (p <0.05), calcium by 4.7%. 10 days after the start of the task of the nanopreparation, the sodium content in the blood cells of the cows of HF and a weak type of GNI becomes less than only 8.7% (p <0.05) and 15.1% (p <0.05) compared with the cows with SVR type GNI.The sodium content in blood cells 30 days after the start of studies in cows of HF and a weak type of GNI does not signifi cantly diff er from the indicators of cows of the CBP type. However, 45 days after the Germatsink feed supplement was prescribed in the blood cells of cows of a weak type of GNI, the content of this metal again becomes signifi cantly higher by 9.2% (p <0.05) of the cows of the SVR type of GNI.In cows with a weak type of higher nervous activity, the serum potassium content within 10 days after the start of the task nanopreparations increases by 16.0% (p <0.05). According to the infl uence of the feed supplement Germacin in the blood cells of sympathetic cows, the ratio of sodium to potassium is normalized. It should be noted that the assignment of the Germacinc feed additive to animals with SVR, SVI and SN type of GNI does not signifi cantly aff ect the potassium transmembrane potential. Whereas the value of this index in cows with a weak type of HNA decreased by 20.9% in 45 days of research (p<0.001). Therefore, the studies shown that the administration of feed additive “Germatsink” to cows with diff erent types of HNA had corrective eff ect on the content of some macronutrients in the blood of animals. Key words: nerve activity, cows, macronutrients, feed additive, blood cells.

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