scholarly journals Evaluation of analytical and statistical approaches for predicting in vitro nitrogen solubility and in vivo pre‐caecal crude protein digestibility of cereal grains in growing pigs

2020 ◽  
Vol 104 (3) ◽  
pp. 965-976
Author(s):  
Pia Rosenfelder‐Kuon ◽  
Jochen Krieg ◽  
Nadja Sauer ◽  
Meike Eklund ◽  
Hanna Katharina Spindler ◽  
...  
Keyword(s):  
Crude Protein ◽  
Nitrogen Solubility ◽  
Protein Digestibility ◽  
Growing Pigs ◽  
Cereal Grains ◽  
Statistical Approaches

scholarly journals Prediction of crude protein digestibility of animal by-product meals for dogs by the protein solubility in pepsin method

2014 ◽  
Vol 3 ◽  
Author(s):  
Iris M. Kawauchi ◽  
Nilva K. Sakomura ◽  
Cristiana F. F. Pontieri ◽  
Aline Rebelato ◽  
Thaila C. Putarov ◽  
...  
Keyword(s):  
Crude Protein ◽  
Pearson Correlation ◽  
Protein Solubility ◽  
Correlation Coefficients ◽  
Basal Diet ◽  
Protein Digestibility ◽  
Large Variability ◽  
The Relationship

AbstractAnimal by-product meals have large variability in crude protein (CP) content and digestibility. In vivo digestibility procedures are precise but laborious, and in vitro methods could be an alternative to evaluate and classify these ingredients. The present study reports prediction equations to estimate the CP digestibility of meat and bone meal (MBM) and poultry by-product meal (PM) using the protein solubility in pepsin method (PSP). Total tract CP digestibility of eight MBM and eight PM samples was determined in dogs by the substitution method. A basal diet was formulated for dog maintenance, and sixteen diets were produced by mixing 70 % of the basal diet and 30 % of each tested meal. Six dogs per diet were used to determine ingredient digestibility. In addition, PSP of the MBM and PM samples was determined using three pepsin concentrations: 0·02, 0·002 and 0·0002 %. The CP content of MBM and PM ranged from 39 to 46 % and 57 to 69 %, respectively, and their mean CP digestibility by dogs was 76 (2·4) and 85 (2·6) %, respectively. The pepsin concentration with higher Pearson correlation coefficients with the in vivo results were 0·0002 % for MBM (r 0·380; P = 0·008) and 0·02 % for PM (r 0·482; P = 0·005). The relationship between the in vivo and in vitro results was better explained by the following equations: CP digestibility of MBM = 61·7 + 0·2644 × PSP at 0·0002 % (P = 0·008; R2 0·126); and CP digestibility of PM = 54·1 + 0·3833 × PSP at 0·02 % (P = 0·005; R2 0·216). Although significant, the coefficients of determination were low, indicating that the models were weak and need to be used with caution.

scholarly journals In vitro solubility of meat and bone meal protein with different pepsin concentrations

2000 ◽  
Vol 30 (3) ◽  
pp. 489-492 ◽  
Author(s):  
Cláudio Bellaver ◽  
Dirceu Luís Zanotto ◽  
Antônio Lourenço Guidoni ◽  
Claudete Hara Klein
Keyword(s):  
Crude Protein ◽  
Protein Digestibility ◽  
Enzyme Concentration ◽  
Asymptotic Model ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Meal Protein ◽  
Protein Origin

In vitro protein digestibility of protein sources has been correlated with in vivo digestibility values. However, factors like protein origin, enzyme used and its concentration, pH and processing have been related with the significance of the correlation between the estimates. To address only the enzyme concentration factor, this paper had the objective of testing pepsin at 0.2, 0.02, 0.002 and 0.0002% using the standard AOAC (1995) procedure. Two meat and bone meals (MBM) with low and high crude protein (CP) content were used to determine the coefficient of solubility of CP in pepsin and HCl (CSCPPEPH). Centrifugation was used to establish the nitrogen (N) in the soluble phase, instead of filtration and analysis of N in the residue. The variance analysis and a non-linear asymptotic model were adjusted. The CSCPPEPH under different pepsin concentrations for the two MBM showed higher solubility discrimination with low pepsin concentration. The level of 0.0002% pepsin is better to predict the CP soluble in MBM. This finding implies the assumption that 0.2% pepsin found in the AOAC is not correct for the purpose of determining the range of solubility in high and low CP content in MBM.

A note on in vivo comparison of a urea-formaldehyde-molasses complex and urea molasses mixes as sole nitrogen supplements to a wheat chaff ration for steers

1983 ◽  
Vol 36 (2) ◽  
pp. 309-311
Author(s):  
D. Lall ◽  
H. P. S. Makkar ◽  
S. S. Negi
Keyword(s):  
Crude Protein ◽  
Urea Formaldehyde ◽  
Nitrogen Retention ◽  
Protein Digestibility ◽  
Urea Derivative ◽  
Protein Nitrogen ◽  
Digestible Crude Protein ◽  
Wheat Chaff

ABSTRACTA urfia-formaldehyde-molasses complex appeared to be a promising slow ammonia-releasing urea derivative in in vitro studies. It was compared with urea-molasses mixtures as a non-protein nitrogen supplement to an ad libitum wheat chaff ration for steers. The significantly lower (P < 0·01) crude protein digestibility and intake of digestible crude protein, and the non-significantly lower nitrogen retention on the urea-formaldehyde-molasses complex ration revealed the superiority of simple urea-molasses mixtures as a non-protein nitrogen supplement.

scholarly journals Relationship between In vivo, In vitro Parameters and Chemical Composition to Predict the Nutritive Value of Some Legume Forages

2021 ◽  
Author(s):  
A. Aïssa ◽  
C. Ben Mustapha ◽  
M.R. Alvir ◽  
M. Hadj Ayed ◽  
I.E.A. Znaïdi ◽  
...  
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Chemical Constituents ◽  
Protein Digestibility ◽  
Insoluble Fraction ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Kinetics Of

Background: Many in vivo and laboratory methods have been used to evaluate ruminant feeds. The objective of this experiment was to determine feed intake, digestibility, kinetics of gas production, in vitro organic matter digestibility (OMDv) and metabolizable energy (ME) of Trifolium alexandrinum (T. alexandrinum) and Medicago sativa (M. sativa) at different maturity stages through in vivo and in vitro methods to elaborate predictive equations from chemical constituents. Methods: In vivo assay was carried out with two groups of five rams male kipped in metabolism cage. Samples of forage, refusal and feces were collected and processed for chemical analysis. In vitro gaz production technique was performed on forage samples.Result: The bud and early bloom stage of M. sativa recorded the highest digestibility values of the chemical component (P less than 0.05). The OMDv of M. sativa was 60.65 and 68.26% (P less than 0.01) for early bloom stage and bud stage, respectively. Crude protein digestibility (CPD) was positively correlated with crude protein rate (R2=0.83, P less than 0.05). The gas production from the insoluble fraction “b” fraction showed a positive correlation with acid detergent fiber (ADF) (R2=0.999, P less than 0.001). However, cumulative gas production at 24h and 48 h of incubation were negatively correlated (P less than 0.05) with the ADF rate (R2= -0.98 and -0.97, respectively). 

Effect of salinomycin and enzyme supplementation on nutrient digestibility and the performance of pigs fed barley- or rye-based diets

1992 ◽  
Vol 72 (1) ◽  
pp. 117-125 ◽  
Author(s):  
P. A. Thacker ◽  
G. L. Campbell ◽  
J. W. D. GrootWassink
Keyword(s):  
Feed Efficiency ◽  
Crude Protein ◽  
Nutritive Value ◽  
Control Diet ◽  
Protein Digestibility ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Beta Glucanase ◽  
Dry Matter Digestibility ◽  
Crossbred Pigs

Two experiments of a factorial design (sex × treatment) were conducted to determine the effects of enzyme and salinomycin supplementation on the nutritive value of barley or rye-based diets for growing pigs fed from approximately 20 to 85 kg. For exp. 1, 72 crossbred pigs were fed either a barley-based control diet or a similar diet supplemented with enzyme (Aspergillus niger; 750 units g−1 beta-glucanase and 650 units g−1 pentosanase), salinomycin (25 ppm) or both additives in combination. For exp. 2, two replicates of 48 pigs were fed either a barley-based diet, an unsupplemented rye-based diet or a rye-based diet supplemented with enzyme, salinomycin or both additives. During both experiments, chromic oxide (0.5%) was added to the diet of four to six pigs/treatment to act as a digestibility indicator starting when the pigs reached 42 kg. Neither enzyme nor salinomycin, alone or in combination, significantly improved the growth rate or feed efficiency of pigs fed barley or rye. Supplementation of barley with the combination of additives significantly (P < 0.05) improved protein digestibility while neither enzyme nor salinomycin had any effect on nutrient digestibility when fed alone. In rye-based diets, salinomycin, both alone and in combination, significantly (P < 0.05) improved the digestibility of crude protein and energy. Dry matter digestibility of the rye-based diets was unaffected by treatment. Key words: Swine, rye, barley, beta-glucanase, pentosanase, salinomycin

The effect of extrusion cooking of different starch sources on the in vitro and in vivo digestibility in growing pigs

2006 ◽  
Vol 131 (1-2) ◽  
pp. 67-86 ◽  
Author(s):  
Tiehu Sun ◽  
Helle Nygaard Lærke ◽  
Henry Jørgensen ◽  
Knud Erik Bach Knudsen
Keyword(s):  
Growing Pigs ◽  
Extrusion Cooking

scholarly journals Nutritive value and metabolic effects of whey protein concentrate and hydrolysed lactose for growing pigs

1984 ◽  
Vol 56 (3) ◽  
pp. 227-238
Author(s):  
Matti Näsi
Keyword(s):  
Amino Acids ◽  
Whey Protein ◽  
Crude Protein ◽  
Nutritive Value ◽  
Mineral Metabolism ◽  
Protein Digestibility ◽  
Growing Pigs ◽  
Metabolic Effects ◽  
Whey Protein Concentrate ◽  
Protein Concentrate

In two digestibility and balance trials with growing pigs, whey protein concentrate (WPC) was compared as a protein supplement with casein (CAS) and dried skim milk (DSM), and, 30 % lactose (40 % dried whey, DW) was compared as a sugar supplement with the same amounts of hydrolysed lactose (HYLA) and sucrose (SUC). The effects of these supplements on protein and mineral metabolism of the pigs were investigated, WPC contained 42.2 % crude protein and had a high content of lysine, 8.6 g, and sulphur containing amino acids: cystine 2.8 and methionine 2.2g/16 g N, These exceeded the values for DSM. The hydrolysing degree of the enzymatically treated lactose syrup was 73 %. WPC had high crude protein digestibility, 99.1 % as compared to 95.4 for CAS and 95.0 % for DSM. Dried whey had low crude protein digestibility, 72.5 %. The amino acids in the WPC diet were highly digestible, but low values were obtained for the DW diet. On the WPC diet, nitrogen retention was higher than with the other protein supplements (P > 0.05), urinary urea excretion was low and the biological value very high. On a combination of WPC and HYLA protein utilisation was higher than on dried whole whey. On the diets supplemented with different sugars, none of the blood parameters differed statistically significantly (P > 0.05) and all values lay within the reference range. Water intake was on average 49 % greater on diets with sugar supplements than without. Urinary excretion of reducing sugars averaged 40.2, 8.3 and 6.6 g/d on the HYLA, SUC and DW diets, while on the diets without sugar supplements the values were 0.8—1.2 g/d. The following mean daily mineral retention values were obtained: P 4.0 g, Ca 5,9 g. Mg 0,4 g, Na 1.9g, K 2.9 g, Fe 27 mg, Cu 6.4 mg, Zn 65 mg and Mn 4.0 mg. The surplus Na and K on the DW diet were excreted in the urine and the pigs did not have diarrhoea.

scholarly journals The influence of the method of preservation of forages on the digestion in dairy cows. 1. Composition of the forages and digestibility of dry matter, organic matter and nitrogen.

1975 ◽  
Vol 23 (1) ◽  
pp. 3-9
Author(s):  
S. Tamminga ◽  
C.J. van der Koelen
Keyword(s):  
Organic Matter ◽  
Formic Acid ◽  
Dairy Cows ◽  
Crude Protein ◽  
Dry Matter ◽  
Mixed Diet ◽  
Apparent Digestibility

1. Grass from the same sward was ensiled without additive, with 14.6 g formic acid/100 g crude protein or 10.8 g formic acid and 10.6 g formaldehyde/100 g crude protein. Similar grass was dried and pelleted. Drying or ensiling with the mixture reduced solubility of N in the preserved grass but formic acid increased it, and ensiling without additive increased it even more. Apparent digestibility of N in the rumen of cows tended to decrease with decrease in solubility. Digestibility in vitro of the mixed diet given to the cows, calculated from digestibility of the separate components, agreed well with the values in vivo for diets with silages, but was high for that with dried grass. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Energy values and protein digestibility of soybean milk by-product in pigs based on in vitro assays

2020 ◽  
Vol 34 (1) ◽  
pp. 73-81
Author(s):  
Jung Yeol Sung ◽  
Bokyung Hong ◽  
Youngeun Song ◽  
Beob Gyun Kim
Keyword(s):  
Protein Content ◽  
Crude Protein ◽  
Protein Digestibility ◽  
Metabolizable Energy ◽  
In Vitro Assays ◽  
Feed Ingredient ◽  
Soybean Milk ◽  
Gross Energy ◽  
Swine Diets

Background: Soybean milk by-product (SMBP) is a potential alternative feed ingredient in swine diets due to its high protein content. However, information on energy and nutritional values of SMBP used as swine feed ingredient is limited. Objective: To estimate energy values and protein digestibility of SMBP in pigs based on in vitro assays. Methods: Four SMBP samples were obtained from 3 soybean milk-producing facilities. In vitro total tract disappearance (IVTTD) and in vitro ileal disappearance (IVID) of dry matter (DM) in the SMBP samples were determined. In vitro ileal disappearance of crude protein was determined by analyzing crude protein content in undigested residues after determining IVID of DM. Digestible and metabolizable energy of SMBP were estimated using gross energy, IVTTD of DM, and prediction equations. Results: Sample 4 had greater IVTTD of DM than that of sample 3 (97.7 vs. 94.4%, p<0.05), whereas IVID of DM in sample 4 was lower compared with sample 1 (53.5 vs. 65.0%, p<0.05). In vitro ileal disappearance of crude protein in sample 2 was greater than that in sample 1 and 3 (92.6 vs. 90.6 and 90.1%; p<0.05). The estimated metabolizable energy of SMBP ranged from 4,311 to 4,619 kcal/kg as-is basis and the value of sample 3 was the least (p<0.05) among SMBP samples. Conclusion: Energy values and protein digestibility should be determined before using SMBP in swine diets.

Sign in / Sign up

Export Citation Format

Share Document