Effects of live yeast (Saccharomyces cerevisiae) and type of cereal on rumen microbial fermentation in a dual flow continuous culture fermentation system

2018 ◽  
Vol 102 (6) ◽  
pp. 1488-1496 ◽  
Author(s):  
Diego Moya ◽  
Alfred Ferret ◽  
Marta Blanch ◽  
Mari Carmen Fuentes ◽  
Jose Ignacio Fandiño ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Continuous Culture ◽  
Microbial Fermentation ◽  
Yeast Saccharomyces Cerevisiae ◽  
Fermentation System ◽  
Live Yeast

scholarly journals The Lipomyces starkeyi gene Ls120451 encodes a cellobiose transporter that enables cellobiose fermentation in Saccharomyces cerevisiae

2020 ◽  
Vol 20 (3) ◽  
Author(s):  
Jorg C de Ruijter ◽  
Kiyohiko Igarashi ◽  
Merja Penttilä
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Heterologous Expression ◽  
In Silico ◽  
Penicillium Oxalicum ◽  
Microbial Fermentation ◽  
Lipomyces Starkeyi ◽  
Yeast Saccharomyces Cerevisiae ◽  
Sugar Transporters ◽  
Selection Of

ABSTRACT Processed lignocellulosic biomass is a source of mixed sugars that can be used for microbial fermentation into fuels or higher value products, like chemicals. Previously, the yeast Saccharomyces cerevisiae was engineered to utilize its cellodextrins through the heterologous expression of sugar transporters together with an intracellular expressed β-glucosidase. In this study, we screened a selection of eight (putative) cellodextrin transporters from different yeast and fungal hosts in order to extend the catalogue of available cellobiose transporters for cellobiose fermentation in S. cerevisiae. We confirmed that several in silico predicted cellodextrin transporters from Aspergillus niger were capable of transporting cellobiose with low affinity. In addition, we found a novel cellobiose transporter from the yeast Lipomyces starkeyi, encoded by the gene Ls120451. This transporter allowed efficient growth on cellobiose, while it also grew on glucose and lactose, but not cellotriose nor cellotetraose. We characterized the transporter more in-depth together with the transporter CdtG from Penicillium oxalicum. CdtG showed to be slightly more efficient in cellobiose consumption than Ls120451 at concentrations below 1.0 g/L. Ls120451 was more efficient in cellobiose consumption at higher concentrations and strains expressing this transporter grew slightly slower, but produced up to 30% more ethanol than CdtG.

scholarly journals Effect of live yeast Saccharomyces cerevisiae supplementation on the performance and cecum microbial profile of suckling piglets

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0219557 ◽  
Author(s):  
Tadele G. Kiros ◽  
Diana Luise ◽  
Hooman Derakhshani ◽  
Renee Petri ◽  
Paolo Trevisi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Saccharomyces Cerevisiae ◽  
Microbial Profile ◽  
Suckling Piglets ◽  
Live Yeast

scholarly journals Quantitative carcass characteristics and quality of shoulder and loin of crossbred goat kids receiving diets with live yeast (Saccharomyces cerevisiae var. boulardii)

2021 ◽  
Vol 42 (6) ◽  
pp. 3515-3526
Author(s):  
Fernanda Maraquena Soares Pili ◽  
◽  
Cibele Regina Schneider ◽  
Caroline Isabela da Silva ◽  
Giovana da Silva Oliveira ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dry Matter ◽  
Live Weight ◽  
Carcass Weight ◽  
Yeast Saccharomyces Cerevisiae ◽  
Slaughter Age ◽  
Qualitative Characteristics ◽  
Compactness Index ◽  
Live Yeast

The objective of this study was to evaluate the influence of live yeast (Saccharomyces cerevisiae var. boulardii) on the quantitative and qualitative characteristics of the shoulder and loin of Boer x Saanen crossbred goat kids. Twenty-seven carcasses were used, 13 females and 14 uncastrated males with an average age of 192 days, weighing 30.76 kg, distributed in a completely randomised design using a 2 x 2 factorial arrangement (diet and sex). The yeast was added to the feed before pelletising in a proportion of 0.050 g/kg dry matter (DM). When the goats reached an average of 30 kg of live weight, they were slaughtered. No effects of live yeast on quantitative carcass and qualitative characteristics of shoulder and loin were observed. Regarding sex, males had higher slaughter weights (31.73 kg), empty carcass weight (27.98 kg), hot carcass weight (14.39 kg), cold carcass weight (14.22 kg), higher carcass compactness index (0.28 kg/cm), lower slaughter age (176 days) and lower cooling loss (1.20%). For the cut yields, females presented a higher loin yield (11.55%) and males presented a higher neck yield (6.84%). In the shoulder, males presented a higher proportion of bone (18.58%), while females presented a higher proportion of fat (17.09%) and muscle:bone (3.47). In the loin, females showed higher fat deposition (19.54%) in relation to males. The inclusion of yeast Saccharomyces cerevisiae var. boulardii in the crossbred goat kids’ diet did not generate differences in the quantitative and qualitative characteristics of the shoulder and loin.

scholarly journals Is Raw Milk Microbiota Influenced by the use of Live Yeast (Saccharomyces cerevisiae) as Ruminant Feed Additive?

2016 ◽  
Vol 11 (3) ◽  
pp. 124-129
Author(s):  
Ester Grilli ◽  
Helene Tormo ◽  
Mattia Fustini ◽  
Chloe Deneufbour ◽  
Marina Losio ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Raw Milk ◽  
Feed Additive ◽  
Yeast Saccharomyces Cerevisiae ◽  
Ruminant Feed ◽  
Live Yeast
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