Functions of pipecolic acid on induced resistance against Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000 in tomato plants

2020 ◽  
Vol 168 (10) ◽  
pp. 591-600
Author(s):  
Huijuan Zhang ◽  
Yating Qiu ◽  
Miao Li ◽  
Fengming Song ◽  
Ming Jiang
Keyword(s):  
Botrytis Cinerea ◽  
Induced Resistance ◽  
Pseudomonas Syringae ◽  
Pipecolic Acid ◽  
Tomato Dc3000 ◽  
Tomato Plants

scholarly journals Hexanoic Acid-Induced Resistance Against Botrytis cinerea in Tomato Plants

2009 ◽  
Vol 22 (11) ◽  
pp. 1455-1465 ◽  
Author(s):  
Begonya Vicedo ◽  
Víctor Flors ◽  
María de la O Leyva ◽  
Ivan Finiti ◽  
Zhana Kravchuk ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Induced Resistance ◽  
Pseudomonas Syringae ◽  
Hexanoic Acid ◽  
Dependent Manner ◽  
Deficient Mutant ◽  
Callose Deposition ◽  
Tomato Plants ◽  
In The Wild ◽  
Ja Signaling

We have demonstrated that root treatment with hexanoic acid protects tomato plants against Botrytis cinerea. Hexanoic acid-induced resistance (Hx-IR) was blocked in the jasmonic acid (JA)-insensitive mutant jai1 (a coi1 homolog) and in the abscisic acid (ABA)-deficient mutant flacca (flc). Upon infection, the LoxD gene as well as the oxylipin 12-oxo-phytodienoic acid and the bioactive molecule JA-Ile were clearly induced in treated plants. However, the basal ABA levels were not altered. Hexanoic acid primed callose deposition against B. cinerea in a cultivar-dependent manner. Treated plants from Ailsa Craig, Moneymaker, and Rheinlands Ruhm showed increased callose deposition but not from Castlemart. Hexanoic acid did not prime callose accumulation in flc plants upon B. cinerea infection; therefore, ABA could act as a positive regulator of Hx-IR by enhancing callose deposition. Furthermore, although hexanoic acid protected the JA-deficient mutant defensless1 (def1), the priming for callose was higher than in the wild type. This suggests a link between JA and callose deposition in tomato. Hence, the obtained results support the idea that callose, oxylipins, and the JA-signaling pathway are involved in Hx-IR against B. cinerea. Moreover our data support the relevance of JA-signaling for basal defense against this necrotroph in tomato. Hexanoic acid also protected against Pseudomonas syringae, indicating a broad-spectrum effect for this new inducer.

scholarly journals Chitosan Oligosaccharide Induces Resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana by Activating Both Salicylic Acid– and Jasmonic Acid–Mediated Pathways

2018 ◽  
Vol 31 (12) ◽  
pp. 1271-1279 ◽  
Author(s):  
Xiaochen Jia ◽  
Haihong Zeng ◽  
Wenxia Wang ◽  
Fuyun Zhang ◽  
Heng Yin
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Induced Resistance ◽  
Pseudomonas Syringae ◽  
Plant Immunity ◽  
Chitosan Oligosaccharide ◽  
Induction Effect ◽  
Tomato Dc3000 ◽  
Expression Of Genes ◽  
Underlying Mechanisms

Chitosan oligosaccharide (COS) is an effective plant immunity elicitor; however, its induction mechanism in plants is complex and needs further investigation. In this study, the Arabidopsis–Pseudomonas syringae pv. tomato DC3000 (hereafter called DC3000) interaction was used to investigate the induction effect and the underlying mechanisms of COS. COS is effective in inducing resistance to DC3000 in Arabidopsis, and our results demonstrate that treatment with COS 3 days before DC3000 inoculation provided the most effective resistance. Disease severity in jar1 (jasmonic acid [JA]-deficient mutant), NahG, and sid2 (salicylic acid [SA]-deficient mutants) suggest both the SA and JA pathways are required for the Arabidopsis response to DC3000. COS pretreatment induced resistance in wild type (WT), jar1, and also, although to a lesser degree, in NahG and sid2 plants, implying that the SA and JA pathways play redundant roles in COS-induced resistance to DC3000. In COS-pretreated plants, expression of genes related to the SA pathway (PR1, PR2, and PR5) and SA content increased in both WT and jar1. Moreover, expression of genes related to the JA pathway (PDF1.2 and VSP2) and JA content both increased in WT and NahG. In conclusion, COS induces resistance to DC3000 in Arabidopsis by activating both SA- and JA-mediated pathways, although SA and JA pathways play redundant roles in this COS-induced resistance.

scholarly journals Tomato Stress-Associated Protein 4 Contributes Positively to Immunity Against Necrotrophic Fungus Botrytis cinerea

2019 ◽  
Vol 32 (5) ◽  
pp. 566-582 ◽  
Author(s):  
Shixia Liu ◽  
Xi Yuan ◽  
Yuyan Wang ◽  
Hui Wang ◽  
Jiali Wang ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Pseudomonas Syringae ◽  
Expression Patterns ◽  
Plant Stress ◽  
Oxygen Species ◽  
Virus Induced Gene Silencing ◽  
Tomato Dc3000 ◽  
Enhanced Resistance ◽  
Stress Associated Protein ◽  
Associated Proteins

Stress-associated proteins (SAPs) are A20 and AN1 domain–containing proteins, some of which play important roles in plant stress signaling. Here, we report the involvement of tomato SlSAP family in immunity. SlSAPs responded with different expression patterns to Botrytis cinerea and defense signaling hormones. Virus-induced gene silencing of each of the SlSAP genes and disease assays revealed that SlSAP4 and SlSAP10 play roles in immunity against B. cinerea. Silencing of SlSAP4 resulted in attenuated immunity to B. cinerea, accompanying increased accumulation of reactive oxygen species and downregulated expression of jasmonate and ethylene (JA/ET) signaling-responsive defense genes. Transient expression of SlSAP4 in Nicotiana benthamiana led to enhanced resistance to B. cinerea. Exogenous application of methyl jasmonate partially restored the resistance of the SlSAP4-silenced plants against B. cinerea. SlSAP4 interacted with three of four SlRAD23 proteins. The A20 domain in SlSAP4 and the Ub-associated domains in SlRAD23d are critical for SlSAP4-SlRAD23d interaction. Silencing of SlRAD23d led to decreased resistance to B. cinerea, but silencing of each of other SlRAD23s did not affect immunity against B. cinerea. Furthermore, silencing of SlSAP4 and each of the SlRAD23s did not affect immunity to Pseudomonas syringae pv. tomato DC3000. These data suggest that SlSAP4 contributes positively to tomato immunity against B. cinereal through affecting JA/ET signaling and may be involved in the substrate ubiquitination process via interacting with SlRAD23d.

scholarly journals Contribution of the non-effector members of the HrpL regulon, iaaL and matE, to the virulence of Pseudomonas syringae pv. tomato DC3000 in tomato plants

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Melissa G. Castillo-Lizardo ◽  
Isabel M. Aragón ◽  
Vivian Carvajal ◽  
Isabel M. Matas ◽  
María Luisa Pérez-Bueno ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Tomato Dc3000 ◽  
Tomato Plants

scholarly journals Induction of Pseudomonas syringae pv. tomato DC3000 MexAB-OprM Multidrug Efflux Pump by Flavonoids Is Mediated by the Repressor PmeR

2011 ◽  
Vol 24 (10) ◽  
pp. 1207-1219 ◽  
Author(s):  
Paola Vargas ◽  
Antonia Felipe ◽  
Carmen Michán ◽  
María-Trinidad Gallegos
Keyword(s):  
Pseudomonas Syringae ◽  
Efflux Pump ◽  
Regulatory Gene ◽  
Multidrug Efflux ◽  
Tomato Dc3000 ◽  
Multidrug Efflux Pump ◽  
Tomato Plants ◽  
In Vivo Experiments

In this study, we have analyzed the expression of the Pseudomonas syringae pv. tomato DC3000 mexAB-oprM efflux pump operon and of the regulatory gene pmeR, and we have investigated the role of the PmeR protein on transcription from both promoters. We demonstrate that mexAB-oprM and pmeR are expressed in vivo at a relatively high and moderate basal level, respectively, which, in both cases, increases in the presence of different flavonoids and other compounds, such as butyl and methylparaben. We show that PmeR is the local repressor of the mexAB-oprM promoter and is able to regulate its own expression. The mechanism for this regulation includes binding to a pseudopalindromic operator site which overlaps both mexAB-oprM and pmeR promoters. We have also proven that flavonoids are able to interact with PmeR and induce a conformational change that interferes with the DNA binding ability of PmeR, thereby modulating mexAB-oprM and pmeR expression. Finally, we demonstrate by in vivo experiments that the PmeR/MexAB-OprM system contributes to the colonization of tomato plants. These results provide new insight into a transcriptional regulator and a transport system that play essential roles in the ability of P. syringae pv. tomato DC3000 to resist the action of flavonoids produced by the host.

Sign in / Sign up

Export Citation Format

Share Document