scholarly journals Expression of Vitis amurensis VaERF20 in Arabidopsis thaliana Improves Resistance to Botrytis cinerea and Pseudomonas syringae pv. Tomato DC3000

2018 ◽  
Vol 19 (3) ◽  
pp. 696 ◽  
Author(s):  
Mengnan Wang ◽  
Yanxun Zhu ◽  
Rui Han ◽  
Wuchen Yin ◽  
Chunlei Guo ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Botrytis Cinerea ◽  
Pseudomonas Syringae ◽  
Vitis Amurensis ◽  
Tomato Dc3000

scholarly journals Ectopic Expression of Grapevine Gene VaRGA1 in Arabidopsis Improves Resistance to Downy Mildew and Pseudomonas syringae pv. tomato DC3000 But Increases Susceptibility to Botrytis cinerea

2019 ◽  
Vol 21 (1) ◽  
pp. 193
Author(s):  
Shanshan Tian ◽  
Xiangjing Yin ◽  
Peining Fu ◽  
Wei Wu ◽  
Jiang Lu
Keyword(s):  
Disease Resistance ◽  
Botrytis Cinerea ◽  
Pseudomonas Syringae ◽  
Broad Spectrum ◽  
Ectopic Expression ◽  
Vitis Amurensis ◽  
Transcriptional Level ◽  
Tomato Dc3000 ◽  
Resistance Function ◽  
Broad Spectrum Resistance

The protein family with nucleotide binding sites and leucine-rich repeat (NBS-LRR) in plants stimulates immune responses caused by effectors and can mediate resistance to hemi-biotrophs and biotrophs. In our previous study, a Toll-interleukin-1(TIR)-NBS-LRR gene cloned from Vitis amurensis “Shuanghong”, VaRGA1, was induced by Plasmopara viticola and could improve the resistance of tobacco to Phytophthora capsici. In this study, VaRGA1 in “Shuanghong” was also induced by salicylic acid (SA), but inhibited by jasmonic acid (JA). To investigate whether VaRGA1 confers broad-spectrum resistance to pathogens, we transferred this gene into Arabidopsis and then treated with Hyaloperonospora arabidopsidis (Hpa), Botrytis cinerea (B. cinerea), and Pseudomonas syringae pv. tomato DC3000 (PstDC3000). Results showed that VaRGA1 improved transgenic Arabidopsis thaliana resistance to the biotrophic Hpa and hemi-biotrophic PstDC3000, but decreased resistance to the necrotrophic B. cinerea. Additionally, qPCR assays showed that VaRGA1 plays an important role in disease resistance by activating SA and inhibiting JA signaling pathways. A 1104 bp promoter fragment of VaRGA1 was cloned and analyzed to further elucidate the mechanism of induction of the gene at the transcriptional level. These results preliminarily confirmed the disease resistance function and signal regulation pathway of VaRGA1, and contributed to the identification of R-genes with broad-spectrum resistance function.

scholarly journals Tomato Stress-Associated Protein 4 Contributes Positively to Immunity Against Necrotrophic Fungus Botrytis cinerea

2019 ◽  
Vol 32 (5) ◽  
pp. 566-582 ◽  
Author(s):  
Shixia Liu ◽  
Xi Yuan ◽  
Yuyan Wang ◽  
Hui Wang ◽  
Jiali Wang ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Pseudomonas Syringae ◽  
Expression Patterns ◽  
Plant Stress ◽  
Oxygen Species ◽  
Virus Induced Gene Silencing ◽  
Tomato Dc3000 ◽  
Enhanced Resistance ◽  
Stress Associated Protein ◽  
Associated Proteins

Stress-associated proteins (SAPs) are A20 and AN1 domain–containing proteins, some of which play important roles in plant stress signaling. Here, we report the involvement of tomato SlSAP family in immunity. SlSAPs responded with different expression patterns to Botrytis cinerea and defense signaling hormones. Virus-induced gene silencing of each of the SlSAP genes and disease assays revealed that SlSAP4 and SlSAP10 play roles in immunity against B. cinerea. Silencing of SlSAP4 resulted in attenuated immunity to B. cinerea, accompanying increased accumulation of reactive oxygen species and downregulated expression of jasmonate and ethylene (JA/ET) signaling-responsive defense genes. Transient expression of SlSAP4 in Nicotiana benthamiana led to enhanced resistance to B. cinerea. Exogenous application of methyl jasmonate partially restored the resistance of the SlSAP4-silenced plants against B. cinerea. SlSAP4 interacted with three of four SlRAD23 proteins. The A20 domain in SlSAP4 and the Ub-associated domains in SlRAD23d are critical for SlSAP4-SlRAD23d interaction. Silencing of SlRAD23d led to decreased resistance to B. cinerea, but silencing of each of other SlRAD23s did not affect immunity against B. cinerea. Furthermore, silencing of SlSAP4 and each of the SlRAD23s did not affect immunity to Pseudomonas syringae pv. tomato DC3000. These data suggest that SlSAP4 contributes positively to tomato immunity against B. cinereal through affecting JA/ET signaling and may be involved in the substrate ubiquitination process via interacting with SlRAD23d.

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