Inhibitory effects of essential oils from Ocimum basilicum and Ocimum gratissimum on Colletotrichum musae : The causal agent of bananas anthracnose

2019 ◽  
Vol 167 (5) ◽  
pp. 257-264 ◽  
Author(s):  
Mireille Agathe Madjouko ◽  
Séverin Nguemezi Tchameni ◽  
Eliane Sonwa Tchinda ◽  
Pierre Michel Dongmo Jazet ◽  
Pamela Noumegna Kamsu ◽  
...  
Keyword(s):  
Essential Oils ◽  
Ocimum Basilicum ◽  
Causal Agent ◽  
Inhibitory Effects ◽  
Ocimum Gratissimum ◽  
Colletotrichum Musae

scholarly journals Biological activities of essential oils and methanol extracts of five Ocimum species against pathogenic bacteria

2013 ◽  
Vol 31 (No. 2) ◽  
pp. 195-202 ◽  
Author(s):  
S. Saha ◽  
T.D. Dhar ◽  
C. Sengupta ◽  
P. Ghosh
Keyword(s):  
Essential Oils ◽  
Antibacterial Activities ◽  
Ocimum Basilicum ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Gram Negative ◽  
Methanol Extracts ◽  
Ocimum Gratissimum ◽  
Ocimum Species ◽  
Pharmaceutical Industries

The essential oils and methanol extracts of Ocimum basilicum L., Ocimum kilimandscharicum Guerke, Ocimum gratissimum L, Ocimum canum Sims, and Ocimum tenuiflorum L. (green type) were examined for their potential antibacterial activities. The chemical composition of essential oils of Ocimum species was analysed by GC-MS. The inhibitory effects of essential oils and methanol extracts were studied on two Gram-positive (Bacillus subtilis, Micrococcus luteus) and five Gram-negative (Pseudomonas aeruginosa, Shigella dysenteriae, Escherichia coli, Vibrio cholera, and Shigella flexneri) bacteria using disc-diffusion method. Minimum inhibitory concentration (MIC) was assessed by micro broth dilution method. The antibacterial test results showed that the essential oils of Ocimum basilicum L., Ocimum kilimandscharicum Guerke, and Ocimum gratissimum L. strongly inhibited the growth of all of the microorganisms studied, especially of the Gram-negative strains, whereas other two essential oils showed moderate activities. The result may suggest that the essential oils of Ocimum possess compounds with antibacterial activities, and therefore could be used as natural preservative ingredients in food and/or pharmaceutical industries.  

Toxicity and gastric tolerance of essential oils from Cymbopogon citratus, Ocimum gratissimum and Ocimum basilicum in Wistar rats

2008 ◽  
Vol 46 (7) ◽  
pp. 2493-2497 ◽  
Author(s):  
P. Fandohan ◽  
B. Gnonlonfin ◽  
A. Laleye ◽  
J.D. Gbenou ◽  
R. Darboux ◽  
...  
Keyword(s):  
Essential Oils ◽  
Wistar Rats ◽  
Ocimum Basilicum ◽  
Cymbopogon Citratus ◽  
Ocimum Gratissimum

scholarly journals EVALUATION OF THE CHEMICAL COMPOSITION OF ESSENTIAL OILS FROM LEAVES OF SUDANESE OCIMUM GRATISSIMUM AND OCIMUM BASILICUM.

2016 ◽  
Vol 4 (10) ◽  
pp. 5-11
Author(s):  
ItmadAwad Elhassan ◽  
◽  
AmiraAhmed Hamed-Elneel ◽  
ReemAhmed Adam. ◽  
◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Ocimum Basilicum ◽  
Ocimum Gratissimum

scholarly journals Control of Sugarcane Smut Disease: Evaluation of the In vitro Fungitoxicity of Three Essential Oils

2020 ◽  
pp. 34-41
Author(s):  
Yao Kouadio Jacques-Edouard ◽  
Kouamé Konan Didier ◽  
Kouamé Koffi Gaston ◽  
Kassi Koffi Fernand Jean-Martial ◽  
N’guessan Aya Carine ◽  
...  
Keyword(s):  
Essential Oils ◽  
Mycelial Growth ◽  
Zingiber Officinale ◽  
Inhibitory Effects ◽  
Cymbopogon Citratus ◽  
Ocimum Gratissimum ◽  
Sporisorium Scitamineum ◽  
Fungitoxic Effect ◽  
Production Areas

The sugarcane smut disease caused by Sporisorium scitamineum occurs in all production areas. In order to contribute to the management of this disease, a study was carried out in vitro conditions. The aim of this study was to evaluate the antifungal efficacy and to determine the inhibitory concentration of the essentials oils of Ocimum gratissimum, Zingiber officinale and Cymbopogon citratus upon two strains B and Z of Sporisorium scitamineum. These oils were tested on mycelial growth of colonies, spores’ production and teliospores germination. The results showed a variable fungitoxic effect of the oils according to doses. Mycelial growth and spores production were totally inhibited (100%) from 1000 ppm by all the oils. The 90% inhibitory concentrations (IC90) obtained were 880.37 ppm, 847.83 ppm and 805.75 ppm for Ocimum gratissimum, Zingiber officinale and Cymbopogon citratus, respectively. The germination of teliospores was completely reduced by the three essential oils from 500 ppm upwards. Propiconazole had strong inhibitory effects at 400 ppm with a very low IC50 and the IC90 ranged from 305 ppm to 323.36 ppm. Moreover, at the level of the strains, no difference in resistance was observed These oils could provide a good means of controlling sugarcane smut.

scholarly journals Synergistic Antimicrobial Activities of Combinations of Vanillin and Essential Oils of Cinnamon Bark, Cinnamon Leaves and Cloves

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1406
Author(s):  
Rita Cava-Roda ◽  
Amaury Taboada-Rodríguez ◽  
Antonio López-Gómez ◽  
Ginés Benito Martínez-Hernández ◽  
Fulgencio Marín-Iniesta
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Food Packaging ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Inhibitory Effects ◽  
Cinnamon Bark ◽  
E Coli ◽  
Pure Compounds ◽  
Main Component

Plant bioactive compounds have antimicrobial and antioxidant activities that allow them to be used as a substitute for synthetic chemical additives in both food and food packaging. To improve its sensory and bactericidal effects, its use in the form of effective combinations has emerged as an interesting possibility in the food industry. In this study, the antimicrobial activities of essential oils (EOs) of cinnamon bark, cinnamon leaves, and clove and the pure compounds vanillin, eugenol, and cinnamaldehyde were investigated individually and in combination against Listeria monocytogenes and Escherichia coli O157:H7. The possible interactions of combinations of pure compounds and EOs were performed by the two-dimensional checkerboard assay and isobologram methods. Vanillin exhibited the lowest antimicrobial activity (MIC of 3002 ppm against L. monocytogenes and 2795 ppm against E. coli O157:H7), while clove and cinnamon bark EOs exhibited the highest antimicrobial activity (402–404 against L. monocytogenes and 778–721 against E. coli O157:H7). For L. monocytogenes, pure compound eugenol, the main component of cinnamon leaves and clove, showed lower antimicrobial activity than EOs, which was attributed to the influence of the minor components of the EOs. The same was observed with cinnamaldehyde, the main component of cinnamon bark EO. The combinations of vanillin/clove EO and vanillin/cinnamon bark EO showed the most synergistic antimicrobial effect. The combination of the EOs of cinnamon bark/clove and cinnamon bark/cinnamon leaves showed additive effect against L. monocytogenes but indifferent effect against E. coli O157:H7. For L. monocytogenes, the best inhibitory effects were achieved by cinnamon bark EO (85 ppm)/vanillin (910 ppm) and clove EO (121 ppm)/vanillin (691 ppm) combinations. For E. coli, the inhibitory effects of clove EO (104 ppm)/vanillin (1006 ppm) and cinnamon leaves EO (118 ppm)/vanillin (979 ppm) combinations were noteworthy. Some of the tested combinations increased the antimicrobial effect and would allow the effective doses to be reduced, thereby offering possible new applications for food and active food packaging.

Essential oils from Ocimum basilicum cultivars: analysis of their composition and determination of the effect of the major compounds on Haemonchus contortus eggs

2021 ◽  
Vol 95 ◽  
Author(s):  
A.I.P. Sousa ◽  
C.R. Silva ◽  
H.N. Costa-Júnior ◽  
N.C.S. Silva ◽  
J.A.O. Pinto ◽  
...  
Keyword(s):  
Essential Oils ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Ocimum Basilicum ◽  
Alternative Methods ◽  
Egg Hatch ◽  
Plant Essential Oils ◽  
Methyl Chavicol ◽  
The Individual

Abstract The continuous use of synthetic anthelmintics against gastrointestinal nematodes (GINs) has resulted in the increased resistance, which is why alternative methods are being sought, such as the use of natural products. Plant essential oils (EOs) have been considered as potential products for the control of GINs. However, the chemical composition and, consequently, the biological activity of EOs vary in different plant cultivars. The aim of this study was to evaluate the anthelmintic activity of EOs from cultivars of Ocimum basilicum L. and that of their major constituents against Haemonchus contortus. The EOs from 16 cultivars as well the pure compound linalool, methyl chavicol, citral and eugenol were used in the assessment of the inhibition of H. contortus egg hatch. In addition, the composition of three cultivars was simulated using a combination of the two major compounds from each. The EOs from different cultivars showed mean Inhibition Concentration (IC50) varying from 0.56 to 2.22 mg/mL. The cultivar with the highest egg-hatch inhibition, Napoletano, is constituted mainly of linalool and methyl chavicol. Among the individual compounds tested, citral was the most effective (IC50 0.30 mg/mL). The best combination of compounds was obtained with 11% eugenol plus 64% linalool (IC50 0.44 mg/mL), simulating the Italian Large Leaf (Richters) cultivar. We conclude that different cultivars of O. basilicum show different anthelmintic potential, with cultivars containing linalool and methyl chavicol being the most promising; and that citral or methyl chavicol isolated should also be considered for the development of new anthelmintic formulations.

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