Molecular detection and characterization of a stolbur phytoplasma associated with Narcissus tazetta phyllody in Iran

2018 ◽  
Vol 166 (5) ◽  
pp. 372-377 ◽  
Author(s):  
Javad Gholami ◽  
Reza Ranjbar ◽  
Masoud Bahar ◽  
Roya Choupannejad ◽  
Sirvan Saed Moucheshi
Keyword(s):  
Molecular Detection ◽  
Narcissus Tazetta ◽  
Stolbur Phytoplasma

Molecular detection and genetic characterization of ovine gammaherpesvirus-2 (OvHV-2) in sheep in the Philippines

2021 ◽  
Vol 199 ◽  
pp. 106383
Author(s):  
Innina R. Mananguit ◽  
Nest Dale F. Bartolome ◽  
Gabriel Alexis S.P. Tubalinal ◽  
Claro N. Mingala
Keyword(s):  
Molecular Detection ◽  
Genetic Characterization ◽  
The Philippines

scholarly journals MOLECULAR DETECTION AND CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM RAW MILK SOLD IN DIFFERENT MARKETS OF BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 277-282 ◽  
Author(s):  
M. A. Islam ◽  
S. M. L. Kabir ◽  
M. T. Rahman
Keyword(s):  
Molecular Detection ◽  
Culture Media ◽  
Raw Milk ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Selective Media ◽  
Milk Samples ◽  
Antimicrobial Sensitivity ◽  
Antimicrobial Sensitivity Test

The study was intended for molecular detection of S. aureus isolated from raw cow’s milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying cultural properties on different selective media, biochemical tests, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found to be positive for S. aureus. S. aureus specific 16S rRNA gene was amplified from all isolates and identified as S. aureus. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the S. aureus isolates were resistant to amoxicillin (100%), erythromycin (73.33%) and tetracycline (73.33%) but sensitive to azithromycin (93.33%), ciprofloxacin (93.33%), gentamicin (100%), norfloxacin (86.67%) and streptomycin (86.67%).

scholarly journals First molecular detection and genetic characterization of Coxiella burnetii in Zambian dogs and rodents

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Simbarashe Chitanga ◽  
Edgar Simulundu ◽  
Martin C. Simuunza ◽  
Katendi Changula ◽  
Yongjin Qiu ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Molecular Detection ◽  
Genetic Characterization

scholarly journals Molecular Detection and Antibiotic Sensitivity of Salmonella Species Isolated from Goat Feces in Sylhet District of Bangladesh

2021 ◽  
Vol 11 (3) ◽  
pp. 395-401
Author(s):  
Md. Abdus Sabur ◽  
Mouri Rani Das ◽  
Md Bashir Uddin ◽  
Md. Mahfujur Rahman ◽  
Md. Rafiqu Islam ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Antibiotic Sensitivity ◽  
Culture Methods ◽  
Specific Primers ◽  
Antimicrobial Sensitivity ◽  
Conventional Culture ◽  
Antibiotic Sensitivity Test ◽  
One Year ◽  
Pcr Techniques

The present study aimed at the molecular detection of Salmonella species from feces of goats and the characterization of the isolated Salmonella by biochemical and antimicrobial sensitivity techniques. A total of 220 goat feces samples were collected, of which 27 (12.27%) were positive for Salmonella by conventional culture methods and 20 (9.09%) by biochemical and PCR techniques. The prevalence was higher in goats under one year of age (20%), compared to older animals aged one to two years (7.8%) and more than two years of age (4.7%), respectively. Moreover, the prevalence of diarrheic goats was significantly higher (38.46%) than healthy animals (2.76%). DNA was extracted from Salmonella strains and amplified by PCR using the specific primers of Salmonella invasion gene (invA gene). The antibiotic sensitivity test indicated that Ciprofloxacin (100 percent sensitivity), Gentamycin (100 percent sensitivity), and Neomycin (100 percent sensitivity) were the most effective antibiotics for the majority of Salmonella isolates. On the other hand, Salmonella isolates were found to have substantially high resistance to Erythromycin (100%), Amoxicillin (100%), Trimethoprim-Sulfamethoxazole (81.48%), Streptomycin (62.96%), and Tetracycline (55.56 percent). Since the rate of Salmonella carriers was relatively high, eating goat meat could increase the risk of foodborne salmonellosis.

scholarly journals Molecular detection and characterization of Hepatozoon spp. in dogs from the Central part of Turkey

2015 ◽  
Vol 6 (3) ◽  
pp. 388-392 ◽  
Author(s):  
Mehmet Fatih Aydin ◽  
Ferda Sevinc ◽  
Mutlu Sevinc
Keyword(s):  
Molecular Detection
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