Molecular and Biological Characterization of Potato virus Y Isolates from Vietnam

2014 ◽  
Vol 163 (7-8) ◽  
pp. 620-631 ◽  
Author(s):  
Jörg Schubert ◽  
Thomas Thieme ◽  
Ramona Thieme ◽  
Cuong Viet Ha ◽  
Giang Thi Hoang
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Biological Characterization

Biological characterization of six Australian isolates of potato virus Y and their serological detection by ELISA

1987 ◽  
Vol 38 (2) ◽  
pp. 395 ◽  
Author(s):  
R Heath ◽  
RJ Sward ◽  
JR Moran ◽  
AJ Mason ◽  
ND Hallam
Keyword(s):  
Host Plant ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Cultivars ◽  
Biological Characteristics ◽  
Serological Detection ◽  
Biological Characterization ◽  
Indicator Plants ◽  
Necrotic Symptoms

Six potato virus Y isolates from Victoria and Queensland were characterized on the basis of host plant reactions. Four isolates from potato produced symptoms in indicator plants and potato cultivars consistent with those caused by the PVYO group. Two isolates from tobacco produced necrotic symptoms on some tobacco cultivars characteristic of the PVYN group, but did not systemically infect potato cultivars and therefore could not be considered to belong to any of the previously described PVY strain groups. Despite the wide variation in their biological characteristics, an antiserum produced to one of the potato isolates was able to detect all six of the isolates when used in ELISA

scholarly journals Molecular and biological characterization of Potato virus Y detected in zucchini in China

VirusDisease ◽  
2021 ◽  
Author(s):  
Xuefeng Wei ◽  
Hongli Zheng ◽  
Yahan Chen ◽  
Si Zhang ◽  
Zhengnan Li ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Biological Characterization

Molecular and biological characterization of a recombinant isolate of potato virus Y from Mexico

2014 ◽  
Vol 159 (7) ◽  
pp. 1781-1785 ◽  
Author(s):  
Arturo Quintero-Ferrer ◽  
Loreto Robles-Hernandez ◽  
Ana C. Gonzalez-Franco ◽  
Camille Kerlan ◽  
Alexander V. Karasev
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Biological Characterization

A single nucleotide polymorphism-based technique for specific characterization of YO and YN isolates of Potato virus Y (PVY).

2005 ◽  
Vol 125 (1) ◽  
pp. 83-93 ◽  
Author(s):  
Emmanuel Jacquot ◽  
Michel Tribodet ◽  
Flora Croizat ◽  
Valérie Balme-Sinibaldi ◽  
Camille Kerlan
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide

scholarly journals Detection and molecular characterization of Slovak tomato isolates belonging to two recombinant strains of potato virus Y

2016 ◽  
Vol 60 (04) ◽  
pp. 347-353 ◽  
Author(s):  
N. SIHELSKÁ ◽  
L. PREDAJŇA ◽  
A. NAGYOVÁ ◽  
K. ŠOLTYS ◽  
J. BUDIŠ ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Recombinant Strains

scholarly journals Detection and genome characterization of Potato virus Y isolates infecting potato (Solanum tuberosum L.) in La Union (Antioquia, Colombia)

2016 ◽  
Vol 34 (3) ◽  
pp. 317-328 ◽  
Author(s):  
Pablo Gutiérrez S. ◽  
Mauricio Marín M. ◽  
Daniel Muñoz E.
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Seed Tuber ◽  
Rt Pcr ◽  
Potato Plants ◽  
Nextgeneration Sequencing ◽  
Prevalent Strain ◽  
Detailed Molecular Analysis

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity >99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.

Characterization of a Novel Far-Eastern Potato Virus Y Isolates

2009 ◽  
Vol 8 (2) ◽  
pp. 62-67 ◽  
Author(s):  
Yury G. Volkov ◽  
Nadezhda N. Kakareka ◽  
Larissa A. Balabanova ◽  
Zinaida N. Kozlovskay ◽  
Mikhail V. Sapotsky
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Far Eastern
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