Biological characterization of six Australian isolates of potato virus Y and their serological detection by ELISA

1987 ◽  
Vol 38 (2) ◽  
pp. 395 ◽  
Author(s):  
R Heath ◽  
RJ Sward ◽  
JR Moran ◽  
AJ Mason ◽  
ND Hallam
Keyword(s):  
Host Plant ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Cultivars ◽  
Biological Characteristics ◽  
Serological Detection ◽  
Biological Characterization ◽  
Indicator Plants ◽  
Necrotic Symptoms

Six potato virus Y isolates from Victoria and Queensland were characterized on the basis of host plant reactions. Four isolates from potato produced symptoms in indicator plants and potato cultivars consistent with those caused by the PVYO group. Two isolates from tobacco produced necrotic symptoms on some tobacco cultivars characteristic of the PVYN group, but did not systemically infect potato cultivars and therefore could not be considered to belong to any of the previously described PVY strain groups. Despite the wide variation in their biological characteristics, an antiserum produced to one of the potato isolates was able to detect all six of the isolates when used in ELISA

Molecular and Biological Characterization of Potato virus Y Isolates from Vietnam

2014 ◽  
Vol 163 (7-8) ◽  
pp. 620-631 ◽  
Author(s):  
Jörg Schubert ◽  
Thomas Thieme ◽  
Ramona Thieme ◽  
Cuong Viet Ha ◽  
Giang Thi Hoang
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Biological Characterization

scholarly journals Molecular and biological characterization of Potato virus Y detected in zucchini in China

VirusDisease ◽  
2021 ◽  
Author(s):  
Xuefeng Wei ◽  
Hongli Zheng ◽  
Yahan Chen ◽  
Si Zhang ◽  
Zhengnan Li ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Biological Characterization

scholarly journals Biological and molecular characterization of various isolates  of Potato virus Y-N (PVY-N) strain group

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 278-280
Author(s):  
J. Ptáček ◽  
P. Dědič ◽  
J. Matoušek
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Molecular Genetic ◽  
Rt Pcr ◽  
Nucleotide Level ◽  
Tobacco Plants ◽  
Specific Identification ◽  
Pcr Products ◽  
Necrotic Symptoms

Fourteen Potato virus Y (PVY) isolates were characterized. They represented PVYN strain only. However, application of serological and molecular genetic methods led to a more complicated characterization. For example, five isolates induced necrotic symptoms on tobacco plants typical of PVY<sup>N</sup>, despite reacting as PVY<sup>O</sup> serologically. Moreover, the PVY isolates were not identical according to molecular genetic properties. Typical PVY<sup>NTN</sup> PCR products were observed for 11 isolates, but four of them (Hr220-5, Hr387-7, Nord 242 and Syn1Scot) did not produce potato tuber necrotic symptoms in infected cultivars. An immunocapture RT-PCR probing was developed using a set of 24 primer pairs derived from eight regions of the PVY genome. Using this method, five out of seven PVY<sup>NTN</sup> isolates including the Czech standard PVY<sup>NTN</sup> from the potato cv. Nicola were found to be identical. However, two PVY<sup>NTN</sup> isolates and all the other probed PVY samples showed unique patterns, suggesting specific differences at the nucleotide level. This method enabled specific identification of individual isolates variability even within different PVY strains.

Molecular and biological characterization of a recombinant isolate of potato virus Y from Mexico

2014 ◽  
Vol 159 (7) ◽  
pp. 1781-1785 ◽  
Author(s):  
Arturo Quintero-Ferrer ◽  
Loreto Robles-Hernandez ◽  
Ana C. Gonzalez-Franco ◽  
Camille Kerlan ◽  
Alexander V. Karasev
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Biological Characterization

Molecular and biological characterization of recombinant isolates of Potato virus Y circulating in potato fields in Mexico

Plant Disease ◽  
2020 ◽  
Author(s):  
Mariana Rodriguez-Rodriguez ◽  
Arturo Quintero-Ferrer ◽  
Kelsie Green ◽  
Loreto Robles-Hernández ◽  
Ana C González-Franco ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Production ◽  
Potato Cultivars ◽  
Mitigation Strategies ◽  
Hypersensitive Resistance ◽  
Multiple Introductions ◽  
Vein Necrosis ◽  
Production Areas

Potato virus Y (PVY) is a significant threat to potato production in Mexico. The presence of recombinant strains of PVY circulating in potato has been reported in the country, but no systematic study on the genetic diversity of PVY in potato and prevalence of PVY strains has been conducted yet. Here, we report on a series of surveys in seed potato production areas in two states in Mexico, Chihuahua and Jalisco, between 2011 and 2019. PVY was detected through the period of nine years in multiple potato cultivars in both states, often remaining asymptomatic in the most popular cultivars, such as Fianna and Agata. When typed to strain, all PVY samples studied were found to have N-serotype, and were all identified molecularly as isolates of the same recombinant strain, PVYNTN. Five of these PVY isolates were tested on tobacco, where they induced vein necrosis supporting the molecular typing. This identification was also confirmed biologically on differential potato cultivars, where one PVYNTN isolate from the 2013 survey triggered the hypersensitive resistance conferred by the Nztbr gene in the cv. Maris Bard. Seven of these Mexican PVYNTN isolates, collected between 2013 and 2019, including two PVY isolates from potato tubers exhibiting potato tuber necrotic ringspot disease, were subjected to whole genome sequencing and found to show a typical PVYNTNa recombinant structure. When subjected to phylogenetic analysis, Mexican PVYNTN sequences clustered in more than three separate clades, suggesting multiple introductions of PVYNTN in the country. The wide circulation of the PVYNTN strain in Mexican potato should be taken into account by the potato producers, to develop mitigation strategies for this PVY strain associated with tuber necrotic symptoms.

A single nucleotide polymorphism-based technique for specific characterization of YO and YN isolates of Potato virus Y (PVY).

2005 ◽  
Vol 125 (1) ◽  
pp. 83-93 ◽  
Author(s):  
Emmanuel Jacquot ◽  
Michel Tribodet ◽  
Flora Croizat ◽  
Valérie Balme-Sinibaldi ◽  
Camille Kerlan
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide
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