Prepenetration and Penetration of Colletotrichum gloeosporioides into Guava Fruit (Psidium guajava L.): Effects of Temperature, Wetness Period and Fruit Age

2014 ◽  
Vol 163 (3) ◽  
pp. 149-159 ◽  
Author(s):  
Sylvia Raquel Gomes Moraes ◽  
Maria Eugenia Escanferla ◽  
Nelson Sidnei Massola
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Psidium Guajava ◽  
Guava Fruit ◽  
Wetness Period ◽  
Effects Of Temperature

scholarly journals Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.)

2013 ◽  
Vol 35 (2) ◽  
pp. 657-664 ◽  
Author(s):  
Sylvia Raquel Gomes Moraes ◽  
Francisco André Osama Tanaka ◽  
Nelson Sidnei Massola Júnior
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Colletotrichum Gloeosporioides ◽  
Psidium Guajava ◽  
Mature Stage ◽  
Guava Fruit ◽  
Transmission Electron ◽  
Colonization Process ◽  
Parenchymal Cells ◽  
Appressoria Formation

Anthracnose, caused by Colletotrichum gloeosporioides, produces brown lesions on guava fruits, causing severe losses on postharvest. In this study, the infection and colonization of guava fruits by C. gloeosporioides has been examined using scanning and transmission electron microscopy. Fruits at the physiologically mature stage were inoculated with a 10(5) conidia/mL spore suspension. Afterward, fruits were incubated at 25 °C in a wet chamber for periods of 6, 12, 24, 48, 96 and 120 h to allow examination of the infection and colonization process. Conidia germination and appressoria formation occurred six hours after inoculation (h.a.i). Penetration occurred directly via penetration pegs from appressoria, which penetrated the host cuticle 48 h.a.i. Notably, the appressoria did not produce an appressorial cone surrounding the penetration pore. Infection vesicles were found in epidermal cells 96 h.a.i. The same fungal structures were found in epidermal and parenchymal cells of the host 120 h.a.i. Colonization strategy of C. gloeosporioides on guava fruit was intracellular hemibiotrophic.

scholarly journals Quiescent infections by Colletotrichum gloeosporioides in the different development stages of the guava fruit (Psidium guajava L.) in two production seasons

2021 ◽  
Vol 38 (4) ◽  
pp. 751-770
Author(s):  
Clemencia Guédez ◽  
Dorian Rodríguez
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Block Design ◽  
Psidium Guajava ◽  
Reproductive Organs ◽  
Conidial Suspension ◽  
Postharvest Diseases ◽  
Flower Buds ◽  
Development Stages ◽  
Guava Fruit ◽  
Randomized Block Design

The fungus Colletotrichum gloeosporioides is the cause of anthracnose, one of the most important postharvest diseases in guava fruits. The objective of detect quiescent infections caused by this pathogen in flower buds (YF) and guava fruits in two production seasons, thirteen plants and fifty YF per plant were selected, organized under a randomized block design with three treatments: YF inoculated covers, YF covered not inoculated and YF uncovered not inoculated (control). Ten YF were inoculated weekly with 3 mL of conidial suspension of C. gloeosporioides by YF and were identified with colored ribbons.week-1.date-1. One week after the inoculation, five YF were collected per treatment, which were transferred to the laboratory where they were washed, disinfected, incubated in a humid chamber and sown in the PDA medium, to detect the presence of the fungus, the remaining five YF were left in the plant until harvest. Quiescent infections manifested in reproductive organs of all ages, with the highest incidence between 6 and 13 weeks with significant differences (p<0.001) between infections/weeks. The fungus developed more frequently in the humid chamber (69.79 %) with the appearance of an orange mucilaginous mass and in the PDA (51 %) with the presence mycelial growth. The production season was decisive in the detection of quiescent infections, with the highest percentage between april and august (69.10 %) when the highest rainfall occurred. These results demonstrated that C. gloeosporioides infects guava flowers and unripe fruits and the disease manifests itself in ripe fruits.

Guava (Psidium guajava L.) Fruit Coating with Gum – Arabic for Quality and Fruit Fly Control

1970 ◽  
pp. 01-04
Author(s):  
Esameldin B. M. Kabbashi, Ghada H. Abdelrahman and Nawal A. Abdlerahman
Keyword(s):  
Shelf Life ◽  
Gum Arabic ◽  
Fruit Fly ◽  
Psidium Guajava ◽  
Postharvest Quality ◽  
Guava Fruit ◽  
Fruit Shelf Life ◽  
Fly Control ◽  
And Control ◽  
Subtropical Fruit

Guava (Psidium guajava L.) is a lovely tropical and subtropical fruit that originates in Mexico, Central America, and then taken to other distant and near parts around the world. In Sudan this popular fruit is produced in orchards and household and is so profitable but yet attacked by a lot of fruit fly species of the Genera Ceratitis and Bactrocera and the result is a loss of more than 70%. This research aimed at evaluating the effect of Gum Arabic coating (GAC) in extending the shelf life of guava fruit and disinfesting it from these notorious pests. Guava fruits from Kadaro orchards, Khartoum North, were tested using seven concentrations of Gum Arabic solutions. The results reflect that 1: 4 (25%) and 1: 8 (12.5%) (GA: water) concentrations attained 56 and 40% disinfestation, respectively whereas the other lower concentrations effected corresponding results in a range from 20 – 08%. The reduction in maggots per test fruit reached upto 188% as compared to the control.  The highest concentrations (1: 4 & 1: 8) effected a sustainability of 52% in fruit firmness (FF) with an average of medium (3) FF compared to soft FF (4) in the control. The corresponding results in other lower concentrations (1: 16; 1: 32; 1: 64; 1: 72 & 1: 96) were 36, 24, 24, 20 and 16%, respectively. In addition to an average FF of 4 (soft) for all these concentrations and 5 (very soft) for all the corresponding controls. Nevertheless, the sustainability of fruit color (FC) effected by the test concentrations was 52, 44, 24, 22, 24, 20, and 24%, respectively. Regarding these results, the two highest test concentrations effected a sizeable disinfestation and control of fruit flies and a good extension of shelf life of guava in Khartoum State. These findings support using this treatment as an effective IPM tool to extend guava fruit shelf life and upgrading its postharvest quality.

scholarly journals Bioatividade de indutores de resistência no manejo da antracnose da goiabeira (Psidium guajava L.)

2016 ◽  
Vol 42 (2) ◽  
pp. 149-154 ◽  
Author(s):  
Rommel dos Santos Siqueira Gomes ◽  
Andréa Celina Ferreira Demartelaere ◽  
Luciana Cordeiro do Nascimento ◽  
Wendel Oliveira Maciel ◽  
Danilo Bruno Néri da Silva Wanderley
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Psidium Guajava

RESUMO A cultura da goiaba apresenta perdas em torno de 40 a 60% na pós-colheita relacionadas a problemas fitossanitários, como a antracnose causada pelo fungo Colletotrichum gloeosporioides. Pesquisas com métodos alternativos utilizando indutores de resistência têm sido bastante promissoras na busca de produtos com alto potencial no controle de patógenos em pós-colheita. Assim, o presente trabalho teve como objetivo avaliar os indutores de resistência no controle da antracnose e seus efeitos na qualidade físico-química em frutos de goiabeira ‘Paluma’. Os tratamentos utilizados foram: Agro-mós®, Ecolife®, Fosfitonova K®, Cuprogarb 500®, Rocksil® e testemunha (água destilada esterilizada). Realizou-se o teste in vitro, com discos de colônia de C. gloeosporioides (0,45 cm de diâmetro) com sete dias de cultivo e, postos em meio de cultura BDA acrescidos com os indutores. Foi determinada a porcentagem de inibição do crescimento micelial de C. gloeosporioides. O ensaio in vivo foi realizado com frutos de goiabeira variedade Paluma, desinfestados e tratados com indutores. Em seguida, discos de colônia do C. gloeosporioides foram inoculados e do primeiro ao oitavo dia após a inoculação, foram feitas avaliações do diâmetro das lesões nos frutos. As análises pós-colheita foram realizadas, avaliando a perda de massa fresca, sólidos solúveis totais (SST), acidez titulável (AT), razão SST/AT e potencial hidrogeniônico (pH). Foi utilizado o delineamento inteiramente casualizado, em esquema de parcela subdivididas 6x8 (tratamentos x período de avaliação) e as médias comparadas pelo teste de Tukey a 5% de probabilidade. Os indutores Agro-mos®, Ecolife®, Fosfitonova K®, Cuprogarb 500® e Rocksil® inibiram o crescimento micelial do C. gloeosporioides in vitro, reduziram o diâmetro das lesões e mantiveram a qualidade pós-colheita em frutos de goiabeira ‘Paluma’.

scholarly journals First Report of Guignardia psidii, an Ascigerous State of Phyllosticta psidiicola, Causing Fruit Rot on Guava in Venezuela

Plant Disease ◽  
2005 ◽  
Vol 89 (7) ◽  
pp. 773-773 ◽  
Author(s):  
M. S. González ◽  
A. Rondón
Keyword(s):  
Psidium Guajava ◽  
Potato Dextrose Agar ◽  
Fruit Rot ◽  
Symptom Development ◽  
First Report ◽  
Guava Fruit ◽  
Pathogenicity Tests ◽  
Apical Appendage ◽  
Ascigerous State ◽  
Gelatinous Envelope

During August 2003, guava fruit (Psidium guajava L.) cv. Red Dominicana from Cojedes state in Venezuela showed circular, purple-to-brown lesions (0.5 to 1.0 cm) that spread over all surfaces and became black and shrunken on severely affected fruit. Symptomatic tissues were plated aseptically on potato dextrose agar (PDA). Colonies that were initially gray and turned black with age were consistently isolated. The fungus was characterized by dense, submerged, brown-to-black mycelium with septate hyphae. Ascocarps were perithecial, abundant, granulose, subglobose to cylindric obpyriform, solitary or aggregated, mostly unilocular with prominent long necks; ascocarp walls were stromatic, composed of several layers of cells, thick walled, and deeply pigmented on the outside. Asci were subclavate to cylindrical, stipitate, 44 to 84 × 7 to 9 μm, and eight-spored; asci walls were thick and bitunicate. Ascospores were unicellular, hyaline, guttulate, fusiform ellipsoid, widest in the mid-region with rounded ends and gelatinous plugs, and 12 to 17 × 4.5 μm. Conidiomata were pycnidial, intermixed among ascocarps, variable in shape, dark brown, solitary or aggregated, ostiolate, and with long necks up to 1 mm. Pycnidial walls were pseudoparenchymatic, multicellular, and composed of many layers of brown compressed cells. Conidiogenous cells were hyaline, subglobose to cylindrical, and smooth, and holoblastic. Conidia were hyaline, unicellular, obovate, 6 to 12 (7.5) × 5 to 8 μm, slightly truncate at the bases, rounded at apices, guttulate, and provided a gelatinous envelope and apical appendage. Appendages were hyaline, tubular, smooth, and 3.0 to 4.5 × 0.5 μm. The fungus is homothallic because single ascospores and single conidia developed ascigerous states. The ascigerous state was identified as Guignardia psidii (1) and the anamorph as Phyllosticta psidiicola (1,2). Pathogenicity tests were conducted on detached fruits inoculated with monosporic cultures. Pathogenesis and symptom development only occurred when a mixture of mycelium, ascospores, and conidia was used as inoculum. The fungus was reisolated from symptomatic fruit tissues. To our knowledge, this is the first report of Guignardia psidii, an ascigerous state of Phyllosticta psidiicola from guava fruits in Venezuela. References: (1) B. A. Ullasa and R. D. Rawal. Curr. Sci. 53:435, 1984. (2) H. A. van der Aa. Page 95 in: No. 5, Stud. Mycol., 1973.

PCR detection of Colletotrichum gloeosporioides in Psidium guajava

2018 ◽  
Vol 47 (1) ◽  
pp. 95-100 ◽  
Author(s):  
Jinai Yao ◽  
Chengzhong Lan ◽  
Peng Huang ◽  
Deyi Yu
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Psidium Guajava ◽  
Pcr Detection

scholarly journals Peramalan Volume Penjualan Buah Jambu Biji Merah (Psidium Guajava Linn.) di CV Moena Abadi Sejahtera 1

2017 ◽  
Author(s):  
IDA AYU PREMAYANTI ◽  
I WAYAN WIDYANTARA ◽  
RATNA KOMALA DEWI
Keyword(s):  
Citrus Fruit ◽  
Psidium Guajava ◽  
Coefficient Of Determination ◽  
Sales Volume ◽  
Model Function ◽  
Function Model ◽  
Independent Variables ◽  
Guava Fruit ◽  
Forecasting Method ◽  
Exponential Trend

Sales Volume Forecasting Red Guava Fruit (Psidium Guajava Linn.)in CV Moena Abadi Sejahtera 1This study aims to determine the factors that affect the sales volume of fruit Guava Red and estimating sales volume Guava fruit Red 2016, 2017, and 2018 in CV Moena Abadi Sejahtera 1. The data were taken within the last six years the year 2010 until 2015. this study used two models of the sales function is a linear function of sales and sales functions are transformed into a form logharitma tested with three criteria to get the best sales function model. The independent variables that affect the price of fruit Guava Red (PJBM), the price of fruit Bark (PSB), the price of fruit Ambon Banana (PPA), the price fruit Lumajang Oranges (PJL), and the price fruits Kintamani oranges (PJK). Sales function model which is transformed into the shape of a model function logharitma valid sales are LogQJBM = -7.267 - 0,227 log PJBM + 1,798 log PSB - 0,102 log PPA + 0,136 log PJL + 0,379 log PJK ± e. Factors that influence is Red Guava fruit prices, the price of fruit Salak Bali, Lumajang Citrus fruit prices, and the price Citrus fruits Kintamani. Estimated sales of Red Guava fruit using trend analysis and multiple linear regression to see the value of the coefficient of determination (R2), the largest and the value of the standard error (SE), the smallest so get the best forecasting method. Methods exponential trend is the best forecasting method is Y = 502.34 + 1.0023 t ± e. The estimation results of the Red Guava fruit sales in 2016, 2017, and 2018 continue to rise. CV Moena Abadi Sejahtera 1 should use a forecasting method to maintain the availability of the fruit in the store, especially the Red Guava fruit.

scholarly journals Efeito in vitro de compostos de plantas sobre o fungo Colletotrichum gloeosporioides Penz: isolado do maracujazeiro

2009 ◽  
Vol 33 (spe) ◽  
pp. 1853-1860 ◽  
Author(s):  
André Costa da Silva ◽  
Nilza de Lima Pereira Sales ◽  
Alisson Vinícius de Araújo ◽  
Cecílio Frois Caldeira Júnior
Keyword(s):  
Manihot Esculenta ◽  
Colletotrichum Gloeosporioides ◽  
Curcuma Longa ◽  
Psidium Guajava ◽  
Cymbopogon Citratus ◽  
Lippia Citriodora ◽  
Eclipta Alba ◽  
Lippia Sidoides ◽  
Solanum Sisymbriifolium

Neste trabalho, objetivou-se avaliar o efeito de extratos e óleos essenciais de quatorze plantas medicinais e ou nativas, conhecidas popularmente no Norte de Minas Gerais, sobre a germinação de esporos e crescimento micelial do fungo Colletotrichum gloeosporioides. Foram utilizados no teste de inibição do crescimento micelial, os extratos aquosos de alecrim (Rosmarinus officinalis), erva baleeira (Cordia verbenacea), joá (Solanum sisymbriifolium), quebra-pedra (Phyllanthus corcovadensis), erva botão (Eclipta alba) e açafrão da índia (Curcuma longa) obtidos por meio do processo de infusão; óleo essencial de alecrim de vargem (Família Lamiaceae), alecrim pimenta (Lippia sidoides), alfavaca cravo (Ocimum gratissimum), lippia (Lippia citriodora), goiaba branca (Psidium guajava), capim santo (Cymbopogon citratus); óleo resina de copaíba (Copaifera langsdorffi); hidrolatos de alecrim de vargem, goiaba vermelha (Psidium guajava), lippia, capim santo, goiaba branca, alfavaca cravo; manipueira de (Manihot esculenta). Também foram testados esses mesmos hidrolatos e os óleos essenciais na germinação dos esporos do fungo. Todos os tratamentos foram realizados in vitro, cultivando-se o fungo em meio de cultura BDA acrescido de 100μL dos compostos vegetais. No teste de inibição da germinação, todos os óleos essenciais impediram a germinação do fungo. No entanto, os hidrolatos não tiveram esse efeito. No teste de crescimento micelial, os óleos essenciais de todas as plantas inibiram completamente o crescimento do fungo, exceto o óleo da goiaba branca. Os extratos aquosos, a manipueira, o óleo resina e os hidrolatos, também não foram eficientes sobre o crescimento do patógeno. Esses resultados indicam o potencial antifúngico de alguns óleos essenciais de plantas medicinais.

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