Selective Laser Melted Titanium Alloy for Transgingival Components: Influence of Surface Condition on Fibroblast Cell Behavior

2021 ◽  
Author(s):  
Marie‐Joséphine Crenn ◽  
Aurélie Benoit ◽  
Géraldine Rohman ◽  
Thomas Guilbert ◽  
Olivier Fromentin ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Surface Condition ◽  
Fibroblast Cell ◽  
Cell Behavior

Alteration of Fibroblast Cell Behavior due to Contraction of Substrate

2010 ◽  
Author(s):  
Uday Chippada ◽  
Xue Jiang ◽  
Michelle Previtera ◽  
Rene Schloss ◽  
Bernard Yurke ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Fibroblast Cell ◽  
Cellular Responses ◽  
Cell Behavior ◽  
Cellular Behavior ◽  
Polyacrylamide Hydrogels ◽  
3T3 Fibroblasts ◽  
Nih 3T3

Many researchers have utilized hydrogels as substrates for cell attachment. The stiffness of these substrates has been found to influence the cellular behavior such as morphology, proliferation, growth and differentiation. Lo et al. deformed polyacrylamide substrates with a blunted microneedle and observed the movement of NIH 3T3 fibroblasts. In both pulling and pushing, the cells reversed their direction and moved away from the needle. This shows that cellular behavior is also affected by stretching the underlying substrates. In a previous study, Lin et al. have demonstrated the ability to contract DNA-crosslinked polyacrylamide hydrogels (‘DNA gels’ in short) by addition of crosslinks. Jiang et al. have utilized these DNA gels as substrates to observe the cellular responses of L929 and GFP fibroblasts to both static and dynamic substrate compliances.

Fibroblast Cell Behavior on Bound and Adsorbed Fibronectin onto Hyaluronan and Sulfated Hyaluronan Substrates

2005 ◽  
Vol 6 (2) ◽  
pp. 638-645 ◽  
Author(s):  
R. Barbucci ◽  
A. Magnani ◽  
A. Chiumiento ◽  
D. Pasqui ◽  
I. Cangioli ◽  
...  
Keyword(s):  
Fibroblast Cell ◽  
Cell Behavior

scholarly journals Nanotubular Oxide Layers and Hydroxyapatite Coatings on Porous Titanium Alloy Ti13Nb13Zr

2018 ◽  
Vol 18 (4) ◽  
pp. 17-23 ◽  
Author(s):  
M. Supernak-Marczewska ◽  
A. Ossowska ◽  
P. Strąkowska ◽  
A. Zieliński
Keyword(s):  
Titanium Alloy ◽  
Surface Condition ◽  
Porous Titanium ◽  
Biological Processes ◽  
Porous Substrate ◽  
Oxide Layers ◽  
Physical Chemical ◽  
Hydroxyapatite Coatings ◽  
Phosphate Coatings ◽  
Calcium Phosphate Coatings

AbstractThe surface condition of an implant has a significant impact on response occurring at the implant-biosystem border. The knowledge of physical-chemical and biological processes allows for targeted modification of biomaterials to induce a specified response of a tissue. The present research was aimed at development of technology composing of obtaining the nanotube oxide layers on a porous titanium alloy Ti13Nb13Zr, followed by the deposition of phosphate coating. The porous substrate (porosity about 50%) was prepared by a selective laser melting of the Ti13Nb13Zr powder with the SLM Realizer 100 equipment. The nanotubular oxide layers were fabricated by electrochemical oxidation in H3PO4 + 0.3% HF mixture for 30 min. at a constant voltage of 20V. The calcium phosphate coatings were formed by the electrochemically assisted deposition (ECAD). The presence of nanotubular oxide layers with their internal diameters ranging from 30 to 100 nm was observed by SEM (JEOL JSM-7600F). The nanotubes have dimensions that facilitated the deposition of hydroxyapatite.

scholarly journals Cell behavior on silica-hydroxyapatite coaxial composite

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0246256
Author(s):  
Jesús Alberto Garibay-Alvarado ◽  
Ericka Berenice Herrera-Ríos ◽  
Claudia Lucía Vargas-Requena ◽  
Álvaro de Jesús Ruíz-Baltazar ◽  
Simón Yobanny Reyes-López
Keyword(s):  
Tissue Regeneration ◽  
Water Retention ◽  
Structural Characteristics ◽  
Sol Gel ◽  
Average Diameter ◽  
Fibroblast Cell ◽  
Cell Behavior ◽  
Polymeric Fibers ◽  
20 Nm

Progress in the manufacture of scaffolds in tissue engineering lies in the successful combination of materials such as bioceramics having properties as porosity, biocompatibility, water retention, protein adsorption, mechanical strength and biomineralization. Hydroxyapatite (HA) is a ceramic material with lots of potential in tissue regeneration, however, its structural characteristics need to be improved for better performance. In this study, silica-hydroxyapatite (SiO2-HA) non-woven ceramic electrospunned membranes were prepared through the sol-gel method. Infrared spectra, scanning electron microscopy and XRD confirmed the structure and composition of composite. The obtained SiO2-HA polymeric fibers had approximately 230±20 nm in diameter and were then sintered at 800°C average diameter decreased to 110±17 nm. Three configurations of the membranes were obtained and tested in vitro, showing that the composite of SiO2-HA fibers showed a high percentage of viability on a fibroblast cell line. It is concluded that the fibers of SiO2-HA set in a coaxial configuration may be helpful to develop materials for bone regeneration.

scholarly journals Attachment and Growth of Fibroblast Cells on Poly (2-Methoxyethyl Acrylate) Analog Polymers as Coating Materials

Coatings ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 461
Author(s):  
Rubaiya Anjum ◽  
Kei Nishida ◽  
Haruka Matsumoto ◽  
Daiki Murakami ◽  
Shingo Kobayashi ◽  
...  
Keyword(s):  
Fibroblast Cell ◽  
Carbon Chain ◽  
Dermal Fibroblasts ◽  
Cell Behavior ◽  
Carbon Chain Length ◽  
Fibroblast Cells ◽  
Coating Materials ◽  
Subsequent Behavior ◽  
Normal Human ◽  
Normal Human Dermal Fibroblasts

The regulation of adhesion and the subsequent behavior of fibroblast cells on the surface of biomaterials is important for successful tissue regeneration and wound healing by implanted biomaterials. We have synthesized poly(ω-methoxyalkyl acrylate)s (PMCxAs; x indicates the number of methylene carbons between the ester and ethyl oxygen), with a carbon chain length of x = 2–6, to investigate the regulation of fibroblast cell behavior including adhesion, proliferation, migration, differentiation and collagen production. We found that PMC2A suppressed the cell spreading, protein adsorption, formation of focal adhesion, and differentiation of normal human dermal fibroblasts, while PMC4A surfaces enhanced them compared to other PMCxAs. Our findings suggest that fibroblast activities attached to the PMCxA substrates can be modified by changing the number of methylene carbons in the side chains of the polymers. These results indicate that PMCxAs could be useful coating materials for use in skin regeneration and wound dressing applications.

Effect of the chassis parts surface condition from high-strength titanium alloy VT-22 in the process of fatigue tests

2021 ◽  
Vol 2021 (2) ◽  
pp. 45-53
Author(s):  
A. O. Gorpenko ◽  
◽  
O.I. Semenets ◽  
O. M. Doniy ◽  
K.O. Valuiska ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Surface Defects ◽  
Surface Condition ◽  
High Strength ◽  
Surface Damage ◽  
Cylindrical Part ◽  
Fatigue Tests ◽  
Landing Gear ◽  
Premature Failure ◽  
Surface Machining

The research focuses on the influence of the surface condition on the resource of high-strength titanium alloy VT-22 landing gear details during fatigue tests. The tests were performed on special facilities that simulate the workload on a rod detail at the stage of extending and retraction of the landing gear. Fatigue tests were performed on four rods. Rods № 1-3 were destroyed at the lugs level, rod №4 withstood the entire cycle of loads, and was examined in an undamaged state. It was found that the cause of the failure of the rod №1 was axial play formation as a result of bracket lug deformation, which led to shock loads on the lug of the rod №1 during the tests. The destruction of the rod №2 could be caused by the shock axial loads due to changes in the characteristics and load values of the facility on the rod №2. The priority factor influencing the premature failure of the rod №3 was the high risks from surface machining in the most loaded part of the rod №3, namely at the R-junction of the cylindrical part to the lug. The presence of surface defects formed during the manufacturing stage, as well as the presence of deep scratches in the area with high load reduce the life of rod № 3 fivefold compared to the undamaged rod № 4, which had no visible surface defects. Surface damage detected in the non-chromized area of the rods can be eliminated by blasting with subsequent surface polishing, which will provide the required resource of the detail (rod № 4). Keywords: high-strength titanium alloy VT-22, rod, fatigue tests, surface defects, structure of the surface layer.

scholarly journals Enamel matrix derivative improves gingival fibroblast cell behavior cultured on titanium surfaces

2015 ◽  
Vol 20 (4) ◽  
pp. 685-695 ◽  
Author(s):  
Yulan Wang ◽  
Yufeng Zhang ◽  
Dai Jing ◽  
Yang Shuang ◽  
Richard J. Miron
Keyword(s):  
Fibroblast Cell ◽  
Enamel Matrix Derivative ◽  
Cell Behavior ◽  
Gingival Fibroblast ◽  
Enamel Matrix ◽  
Titanium Surfaces ◽  
Matrix Derivative
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