Decreased expression of glutathione S-transferase pi correlates with poorly differentiated grade in patients with oral squamous cell carcinoma

2014 ◽  
Vol 44 (3) ◽  
pp. 193-200 ◽  
Author(s):  
Hai-long Ma ◽  
Cong Yu ◽  
Ying Liu ◽  
Yi-ran Tan ◽  
Jin-ke Qiao ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Glutathione S Transferase ◽  
Poorly Differentiated

scholarly journals Diagnostic and prognostic value of cancer stem cell marker CD44 and soluble CD44 in the peripheral Blood of patients with oral Squamous cell carcinoma

2017 ◽  
Vol 2 (3) ◽  
Author(s):  
Aya K. Khamis ◽  
Hamed A. Fouad ◽  
Hanaa S. Raslan ◽  
Mohamed M. Fata ◽  
Amira I. Fayad
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Peripheral Blood ◽  
Prognostic Value ◽  
Stem Cell Marker ◽  
Soluble Cd44 ◽  
Poorly Differentiated ◽  
Diagnostic And Prognostic Value

BackgroundHead and neck cancer is a major health problem. Recent studies on the pathobiology of oral squamous cell carcinoma (OSCC) have led to the discovery of a small population of cancer cells with a consistent behavior with the features of cancer stem cells (CSCs).  CSCs are required and responsible for initiation, maintenance and recurrence of disease. Molecular markers are commonly used for the identification of CSCs. CD44 is the most reported CSC marker in OSCC.The aim of the study was to evaluate and correlate the expression of CD44 in different histopathological grades of OSCC, as well as to assess the diagnostic and prognostic value of soluble CD44 (CD44sol) in peripheral blood of patients.Materials and methodsFifteen patients with OSCC were included; biopsies were histologically evaluated using haematoxylin and eosin. Serial sections were immunohistochemically stained by monoclonal antibody to CD44. The intensity of immunostaining of CD44 was calculated. Enzyme-linked immunosorbent assay (ELISA) method was used to determine the concentration of CD44sol in the blood serum.ResultsAll grades of OSCC showed membranous immunosignaling of CD44. The well, moderately and poorly differentiated OSCC cases showed weak, moderate and intense positive membranous immunosignaling of CD44 respectively.CD44sol levels were significantly higher in OSCC patients than they were in control groups. Soluble CD44 serum levels were significantly higher in poorly differentiated than they were in moderately and well differentiated.ConclusionCSCs detection in fixed human tissue and CD44sol detection in peripheral blood using ELISA seemed to be a promising method and may have a diagnostic and prognostic value in management of OSCC.

scholarly journals ORAL SQUAMOUS CELL CARCINOMA

2015 ◽  
Vol 22 (01) ◽  
pp. 018-026
Author(s):  
Sofia Ali Syed ◽  
Mulazim Hussain Bukhari ◽  
Riaz Ahmed Warraich ◽  
Fauzia Quadir ◽  
Asifa Iqbal ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Maxillofacial Surgery ◽  
Nucleolar Organizer Regions ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Poorly Differentiated ◽  
Well Differentiated

Background: The aim of this study was to evaluate clinico-pathologicalparameters and the status of argyrophilic nucleolar organizer regions stain in various histologicalgrades of oral squamous cell carcinoma. Materials and Methods: A cross sectional studywas conducted on fifty cases of oral squamous cell carcinoma. The specimen were collectedfrom the department of Oral & Maxillofacial Surgery and processed for hematoxylin and eosinstain and AgNOR stain Pathology Laboratory, King Edward Medical University Lahore. Results:Bidi smoking is associated with oral squamous cell carcinoma. The AgNOR (mAgNOR andpAgNOR) status was significantly low in well differentiated and moderately differentiatedcompared to poorly differentiated oral squamous cell carcinoma (p =0.001). AgNOR size inpoorly differentiated was significantly higher than the AgNOR size in well differentiated oralsquamous cell carcinoma. Similarly the distribution of AgNOR in moderately and poorlydifferentiated oral squamous cell carcinoma was significantly high. The AgNORs index wassignificantly high in poorly differentiated squamous cell carcinoma as compared to welldifferentiated and moderately differentiated squamous cell carcinoma. Conclusions: The useof AgNORs stain is easy, valid and reliable method to assess the histological grading of oralsquamous cell carcinoma and should be used to predict the prognosis of patients.

scholarly journals Correlation of increased numbers of tumor-infiltrating T regulatory, CD19 and CD8 T cells in well differentiated oral squamous cell carcinoma

2018 ◽  
Vol 1 ◽  
pp. e201801004
Author(s):  
Victor Angelo MONTALLI ◽  
Fabrício PASSADOR-SANTOS ◽  
Andresa Borges SOARES ◽  
Elizabeth F. MARTINEZ ◽  
Denise B. R. RODRIGUES ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tumor Antigens ◽  
Tumor Grade ◽  
Inflammatory Cells ◽  
Tissue Microarrays ◽  
Poorly Differentiated ◽  
Well Differentiated

Evidence from cancer patients suggests that increases in T-regulatory lymphocyte (Treg) activity may be associated with poor immune responses against tumor antigens, and contribute to immune dysfunction. Since there have been contradictory reports concerning the physiological roles of the Treg and inflammatory cells in the tumor microenvironment, the inflammatory cell influx was investigated for different malignancygrades of oral squamous cell carcinoma (OSCC). A total of 42 patients with OSCC were selected. Tumors were histologically graded as well, moderately, or poorly differentiated. Tissue microarrays were performed to immunohistochemically measure the expression of CD8, Foxp3, CD19, and IL-10. Significant statistical increases (p<0.05) were observed in the numbers of Foxp3+ and CD8+ inflammatory cells, as well as inIL-10 expression, in the well differentiated tumors, compared to the poorly differentiated tumors. No differences between the three different histological grades were observed in the numbers of CD19+ cells. The relationship between the tumor grade and Treg and CD8 cell recruitment in patients with OSCC underlined the fact that the immune system is deregulated as a consequence of the disease.

scholarly journals The synergy of tobacco and alcohol and glutathione S-transferase θ 1 gene deletion and oral squamous cell carcinoma

2016 ◽  
Vol 20 (3) ◽  
pp. 348
Author(s):  
K Paremala ◽  
Sarah D′ Mello ◽  
RadhikaManoj Bavle ◽  
Soumya Makarla ◽  
M Sudhakara ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Gene Deletion ◽  
Glutathione S Transferase

scholarly journals Human Glutathione S-transferase A1 polymorphism and susceptibility to oral squamous cell carcinoma in Japanese

2005 ◽  
Vol 10 (6) ◽  
pp. 331-334 ◽  
Author(s):  
Yasuhiro Komiya ◽  
Yoshiki Kuroda ◽  
Hiroyuki Nakao ◽  
Katsuyuki Arizono ◽  
Ai Nakahara ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Glutathione S Transferase
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